DCTQ_PSEAE
ID DCTQ_PSEAE Reviewed; 210 AA.
AC Q9HU17;
DT 20-JAN-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=C4-dicarboxylate TRAP transporter small permease protein DctQ {ECO:0000305};
GN Name=dctQ {ECO:0000303|PubMed:21725012};
GN OrderedLocusNames=PA5168 {ECO:0000312|EMBL:AAG08553.1};
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [2]
RP FUNCTION, SUBUNIT, INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=21725012; DOI=10.1128/jb.05074-11;
RA Valentini M., Storelli N., Lapouge K.;
RT "Identification of C(4)-dicarboxylate transport systems in Pseudomonas
RT aeruginosa PAO1.";
RL J. Bacteriol. 193:4307-4316(2011).
CC -!- FUNCTION: Part of the tripartite ATP-independent periplasmic (TRAP)
CC transport system DctPQM involved in C4-dicarboxylates uptake.
CC {ECO:0000269|PubMed:21725012}.
CC -!- SUBUNIT: The complex comprises the extracytoplasmic solute receptor
CC protein DctP, and the two transmembrane proteins DctQ and DctM.
CC {ECO:0000305|PubMed:21725012}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000250|UniProtKB:O07837}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- INDUCTION: Expression is maximal in early exponential growth phase and
CC declines with cell density to reach a plateau in the stationary growth
CC phase. Induced by the C(4)-dicarboxylates succinate, fumarate and
CC malate. Positively regulated by RpoN and the DctB/DctD two-component
CC system. Negatively regulated by DctA. {ECO:0000269|PubMed:21725012}.
CC -!- DISRUPTION PHENOTYPE: The dctA-dctPQM double mutant shows no growth on
CC malate and fumarate and residual growth on succinate.
CC {ECO:0000269|PubMed:21725012}.
CC -!- MISCELLANEOUS: The DctPQM carrier is more efficient than the DctA
CC carrier for the utilization of succinate at micromolar concentrations,
CC whereas DctA is the major transporter at millimolar concentrations.
CC {ECO:0000269|PubMed:21725012}.
CC -!- SIMILARITY: Belongs to the TRAP transporter small permease family.
CC {ECO:0000305}.
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DR EMBL; AE004091; AAG08553.1; -; Genomic_DNA.
DR PIR; A83001; A83001.
DR RefSeq; NP_253855.1; NC_002516.2.
DR RefSeq; WP_003105527.1; NZ_QZGE01000002.1.
DR AlphaFoldDB; Q9HU17; -.
DR SMR; Q9HU17; -.
DR STRING; 287.DR97_2536; -.
DR PaxDb; Q9HU17; -.
DR PRIDE; Q9HU17; -.
DR DNASU; 877772; -.
DR EnsemblBacteria; AAG08553; AAG08553; PA5168.
DR GeneID; 877772; -.
DR KEGG; pae:PA5168; -.
DR PATRIC; fig|208964.12.peg.5416; -.
DR PseudoCAP; PA5168; -.
DR HOGENOM; CLU_086356_3_2_6; -.
DR InParanoid; Q9HU17; -.
DR OMA; LEWPTWI; -.
DR PhylomeDB; Q9HU17; -.
DR BioCyc; PAER208964:G1FZ6-5285-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015740; P:C4-dicarboxylate transport; IMP:PseudoCAP.
DR InterPro; IPR007387; TRAP_DctQ.
DR PANTHER; PTHR35011; PTHR35011; 1.
DR Pfam; PF04290; DctQ; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..210
FT /note="C4-dicarboxylate TRAP transporter small permease
FT protein DctQ"
FT /id="PRO_0000435381"
FT TRANSMEM 13..33
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 77..97
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 113..133
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 160..180
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 210 AA; 22963 MW; 028474887D8C5088 CRC64;
MHALARVWAR LEEGLIAFLL AAMTLVTFVY VVLNNLYTLL YDLADLWEGG NETLLAIGDG
VLTLAQEMTW SNALTKALFA WLIFLGIAYG VRTAGHLGVD VLVKLASRPV QRVLGVIACL
ACLGYAGLLC VASYDWVKTL FIAGIGAEDL DHFGIRQWHI GLIVPVGFAL VFIRFAEILV
RILRNRQTGL GLADEAADAL KLTEHEEPKA
