DCTS_BACSU
ID DCTS_BACSU Reviewed; 535 AA.
AC P96601;
DT 14-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Probable C4-dicarboxylate sensor kinase;
DE EC=2.7.13.3;
GN Name=dctS; Synonyms=ydbF; OrderedLocusNames=BSU04450;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RA Kasahara Y., Nakai S., Lee S., Sadaie Y., Ogasawara N.;
RT "A 148 kbp sequence of the region between 35 and 47 degree of the Bacillus
RT subtilis genome.";
RL Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP FUNCTION, AND GENE NAME.
RC STRAIN=168;
RX PubMed=10708364; DOI=10.1099/00221287-146-2-263;
RA Asai K., Baik S.-H., Kasahara Y., Moriya S., Ogasawara N.;
RT "Regulation of the transport system for C4-dicarboxylic acids in Bacillus
RT subtilis.";
RL Microbiology 146:263-271(2000).
RN [4]
RP FUNCTION.
RX PubMed=11717295; DOI=10.1128/jb.183.24.7365-7370.2001;
RA Kobayashi K., Ogura M., Yamaguchi H., Yoshida K., Ogasawara N., Tanaka T.,
RA Fujita Y.;
RT "Comprehensive DNA microarray analysis of Bacillus subtilis two-component
RT regulatory systems.";
RL J. Bacteriol. 183:7365-7370(2001).
CC -!- FUNCTION: Member of the two-component regulatory system DctS/DctR.
CC Probably activates DctR by phosphorylation (By similarity). Essential
CC for expression of dctP. {ECO:0000250, ECO:0000269|PubMed:10708364,
CC ECO:0000269|PubMed:11717295}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + protein L-histidine = ADP + protein N-phospho-L-
CC histidine.; EC=2.7.13.3;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
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DR EMBL; AB001488; BAA19282.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB12252.1; -; Genomic_DNA.
DR PIR; A69771; A69771.
DR RefSeq; NP_388326.1; NC_000964.3.
DR RefSeq; WP_003234348.1; NZ_JNCM01000031.1.
DR AlphaFoldDB; P96601; -.
DR SMR; P96601; -.
DR STRING; 224308.BSU04450; -.
DR PaxDb; P96601; -.
DR PRIDE; P96601; -.
DR DNASU; 938231; -.
DR EnsemblBacteria; CAB12252; CAB12252; BSU_04450.
DR GeneID; 938231; -.
DR KEGG; bsu:BSU04450; -.
DR PATRIC; fig|224308.179.peg.471; -.
DR eggNOG; COG3290; Bacteria.
DR InParanoid; P96601; -.
DR OMA; NHEHMNK; -.
DR PhylomeDB; P96601; -.
DR BioCyc; BSUB:BSU04450-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0000155; F:phosphorelay sensor kinase activity; IBA:GO_Central.
DR CDD; cd00130; PAS; 1.
DR Gene3D; 3.30.565.10; -; 1.
DR InterPro; IPR003594; HATPase_C.
DR InterPro; IPR036890; HATPase_C_sf.
DR InterPro; IPR005467; His_kinase_dom.
DR InterPro; IPR000014; PAS.
DR InterPro; IPR035965; PAS-like_dom_sf.
DR InterPro; IPR033463; sCache_3.
DR InterPro; IPR029151; Sensor-like_sf.
DR InterPro; IPR004358; Sig_transdc_His_kin-like_C.
DR InterPro; IPR016120; Sig_transdc_His_kin_SpoOB.
DR InterPro; IPR039506; SPOB_a.
DR Pfam; PF02518; HATPase_c; 1.
DR Pfam; PF17203; sCache_3_2; 1.
DR Pfam; PF14689; SPOB_a; 1.
DR PRINTS; PR00344; BCTRLSENSOR.
DR SMART; SM00387; HATPase_c; 1.
DR SMART; SM00091; PAS; 1.
DR SUPFAM; SSF103190; SSF103190; 1.
DR SUPFAM; SSF55785; SSF55785; 1.
DR SUPFAM; SSF55874; SSF55874; 1.
DR SUPFAM; SSF55890; SSF55890; 1.
DR PROSITE; PS50109; HIS_KIN; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cell membrane; Kinase; Membrane; Nucleotide-binding;
KW Phosphoprotein; Reference proteome; Transferase; Transmembrane;
KW Transmembrane helix; Two-component regulatory system.
FT CHAIN 1..535
FT /note="Probable C4-dicarboxylate sensor kinase"
FT /id="PRO_0000074728"
FT TOPO_DOM 1..11
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 12..32
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 33..172
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 173..193
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 194..535
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 213..276
FT /note="PAS"
FT DOMAIN 333..528
FT /note="Histidine kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
FT MOD_RES 336
FT /note="Phosphohistidine; by autocatalysis"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00107"
SQ SEQUENCE 535 AA; 59942 MW; 614BDA8BE8F10CFE CRC64;
MNKKKLSIRW KITILSYILV IFSFLIGGIV LIGNIQHTEE RELKKRLMNT ARTVSEMTEV
KEALARKKQT EAVRHAVEEI RMINEADYIV VMDMNHIRYT HPVSTSIGKK SEGADEEAAF
AEHIYFSEAK GEIGTAVRAF YPVKDQDLNQ IGVVLVGKTL PGIADILLHL KRDIAFIVVL
TLGFGLAGSF LLARHIKKQM FQLEPHEIVR MYEERTATFH SMNEGVIAID NRLVITIFNE
KAKQIFEVQG DLIGKVIWEV LKDSRLPEIV ERNKAVYNEE IRVSGKVIMS SRIPIVMKKK
VIGAVAIFQD RTEAAKMAEE LTGVRNFVEA LRVQNHEHMN KLHTIAGLIQ LGKSEKALQL
AFQASTEQEN VTEFLHRSIQ NDAAAGLLLS KIRRGRELGI AVHIDENSSL QQFPEHVDQH
DIVVLLGNLI ENAFGSFETV QSEDKRIDIS IEQTDDILAI LIEDNGCGIE PTHMPRLYDK
GFTVNKTGGT GYGLYLVKQI IDKGSGTIEV DSHAGQGTSF SIVFPMKGEE AQHGS
