ID   DCUA_ECOL6              Reviewed;         433 AA.
AC   P0ABN6; P04539;
DT   13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT   08-NOV-2005, sequence version 1.
DT   03-AUG-2022, entry version 77.
DE   RecName: Full=Anaerobic C4-dicarboxylate transporter DcuA;
GN   Name=dcuA; OrderedLocusNames=c5220;
OS   Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=199310;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CFT073 / ATCC 700928 / UPEC;
RX   PubMed=12471157; DOI=10.1073/pnas.252529799;
RA   Welch R.A., Burland V., Plunkett G. III, Redford P., Roesch P., Rasko D.,
RA   Buckles E.L., Liou S.-R., Boutin A., Hackett J., Stroud D., Mayhew G.F.,
RA   Rose D.J., Zhou S., Schwartz D.C., Perna N.T., Mobley H.L.T.,
RA   Donnenberg M.S., Blattner F.R.;
RT   "Extensive mosaic structure revealed by the complete genome sequence of
RT   uropathogenic Escherichia coli.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:17020-17024(2002).
CC   -!- FUNCTION: Responsible for the transport of C4-dicarboxylates from the
CC       periplasm across the inner membrane. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000250}; Multi-pass
CC       membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DcuA/DcuB transporter (TC 2.A.13.1) family.
CC       {ECO:0000305}.
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DR   EMBL; AE014075; AAN83642.1; -; Genomic_DNA.
DR   RefSeq; WP_000961959.1; NC_004431.1.
DR   AlphaFoldDB; P0ABN6; -.
DR   STRING; 199310.c5220; -.
DR   EnsemblBacteria; AAN83642; AAN83642; c5220.
DR   GeneID; 67414756; -.
DR   KEGG; ecc:c5220; -.
DR   eggNOG; COG2704; Bacteria.
DR   HOGENOM; CLU_036056_1_1_6; -.
DR   OMA; PKQINYL; -.
DR   BioCyc; ECOL199310:C5220-MON; -.
DR   Proteomes; UP000001410; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015556; F:C4-dicarboxylate transmembrane transporter activity; IEA:InterPro.
DR   InterPro; IPR004668; Anaer_Dcu_memb_transpt.
DR   PANTHER; PTHR36106; PTHR36106; 1.
DR   Pfam; PF03605; DcuA_DcuB; 1.
DR   PIRSF; PIRSF004539; C4-dicrbxl_trns; 1.
DR   TIGRFAMs; TIGR00770; Dcu; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..433
FT                   /note="Anaerobic C4-dicarboxylate transporter DcuA"
FT                   /id="PRO_0000170349"
FT   TRANSMEM        1..18
FT                   /note="Helical"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        19
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        20..37
FT                   /note="Helical"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        38..53
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        54..71
FT                   /note="Helical"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        72..85
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        86..103
FT                   /note="Helical"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        104..132
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        133..147
FT                   /note="Helical"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        148..228
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        229..246
FT                   /note="Helical"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        247..264
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        265..282
FT                   /note="Helical"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        283..292
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        293..310
FT                   /note="Helical"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        311..332
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        333..350
FT                   /note="Helical"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        351..355
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000250"
FT   TRANSMEM        356..373
FT                   /note="Helical"
FT                   /evidence="ECO:0000250"
FT   TOPO_DOM        374..433
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   433 AA;  45751 MW;  049C8D186C83C9AD CRC64;
     MLVVELIIVL LAIFLGARLG GIGIGFAGGL GVLVLAAIGV KPGNIPFDVI SIIMAVIAAI
     SAMQVAGGLD YLVHQTEKLL RRNPKYITIL APIVTYFLTI FAGTGNISLA TLPVIAEVAK
     EQGVKPCRPL STAVVSAQIA ITASPISAAV VYMSSVMEGH GISYLHLLSV VIPSTLLAVL
     VMSFLVTMLF NSKLSDDPIY RKRLEEGLVE LRGEKQIEIK SGAKTSVWLF LLGVVGVVIY
     AIINSPSMGL VEKPLMNTTN AILIIMLSVA TLTTVICKVD TDNILNSSTF KAGMSACICI
     LGVAWLGDTF VSNNIDWIKD TAGEVIQGHP WLLAVIFFFA SALLYSQAAT AKALMPMALA
     LNVSPLTAVA SFAAVSGLFI LPTYPTLVAA VQMDDTGTTR IGKFVFNHPF FIPGTLGVAL
     AVCFGFVLGS FML