DCUA_ECOLI
ID DCUA_ECOLI Reviewed; 433 AA.
AC P0ABN5; P04539; Q2M6G6;
DT 13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Anaerobic C4-dicarboxylate transporter DcuA;
GN Name=dcuA; Synonyms=genA; OrderedLocusNames=b4138, JW5735;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=8131924; DOI=10.1042/bst021342s;
RA Six S., Andrews S.C., Roberts R.E., Unden G., Guest J.R.;
RT "Construction and properties of Escherichia coli mutants defective in two
RT genes encoding homologous membrane proteins with putative roles in
RT anaerobic C4-dicarboxylic acid transport.";
RL Biochem. Soc. Trans. 21:342S-342S(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=7961398; DOI=10.1128/jb.176.21.6470-6478.1994;
RA Six S., Andrews S.C., Unden G., Guest J.R.;
RT "Escherichia coli possesses two homologous anaerobic C4-dicarboxylate
RT membrane transporters (DcuA and DcuB) distinct from the aerobic
RT dicarboxylate transport system (Dct).";
RL J. Bacteriol. 176:6470-6478(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=7610040; DOI=10.1093/nar/23.12.2105;
RA Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.;
RT "Analysis of the Escherichia coli genome VI: DNA sequence of the region
RT from 92.8 through 100 minutes.";
RL Nucleic Acids Res. 23:2105-2119(1995).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-412.
RC STRAIN=W / ATCC 11105 / DSM 1900;
RX PubMed=2987841; DOI=10.1093/nar/13.6.2063;
RA Takagi J.S., Ida N., Tokushige M., Sakamoto H., Shimura Y.;
RT "Cloning and nucleotide sequence of the aspartase gene of Escherichia coli
RT W.";
RL Nucleic Acids Res. 13:2063-2074(1985).
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-264.
RC STRAIN=K12;
RX PubMed=3541901; DOI=10.1042/bj2370547;
RA Woods S.A., Miles J.S., Roberts R.E., Guest J.R.;
RT "Structural and functional relationships between fumarase and aspartase.
RT Nucleotide sequences of the fumarase (fumC) and aspartase (aspA) genes of
RT Escherichia coli K12.";
RL Biochem. J. 237:547-557(1986).
RN [8]
RP SEQUENCE REVISION.
RX PubMed=2656658; DOI=10.1128/jb.171.6.3494-3503.1989;
RA Bell P.J., Andrews S.C., Sivak M.N., Guest J.R.;
RT "Nucleotide sequence of the FNR-regulated fumarase gene (fumB) of
RT Escherichia coli K-12.";
RL J. Bacteriol. 171:3494-3503(1989).
RN [9]
RP TOPOLOGY.
RX PubMed=9733683; DOI=10.1128/jb.180.18.4821-4827.1998;
RA Golby P., Kelly D.J., Guest J.R., Andrews S.C.;
RT "Topological analysis of DcuA, an anaerobic C4-dicarboxylate transporter of
RT Escherichia coli.";
RL J. Bacteriol. 180:4821-4827(1998).
RN [10]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: Responsible for the transport of C4-dicarboxylates from the
CC periplasm across the inner membrane.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the DcuA/DcuB transporter (TC 2.A.13.1) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAE78140.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; X79887; CAA56259.1; -; Genomic_DNA.
DR EMBL; U14003; AAA97037.1; -; Genomic_DNA.
DR EMBL; U00096; AAC77098.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE78140.1; ALT_FRAME; Genomic_DNA.
DR EMBL; X02307; CAA26176.1; ALT_SEQ; Genomic_DNA.
DR EMBL; X04066; CAA27702.1; -; Genomic_DNA.
DR PIR; S56366; QQEC94.
DR RefSeq; NP_418561.1; NC_000913.3.
DR RefSeq; WP_000961959.1; NZ_STEB01000014.1.
DR AlphaFoldDB; P0ABN5; -.
DR BioGRID; 4261707; 13.
DR STRING; 511145.b4138; -.
DR TCDB; 2.A.13.1.1; the c4-dicarboxylate uptake (dcu) family.
DR jPOST; P0ABN5; -.
DR PaxDb; P0ABN5; -.
DR PRIDE; P0ABN5; -.
DR EnsemblBacteria; AAC77098; AAC77098; b4138.
DR EnsemblBacteria; BAE78140; BAE78140; BAE78140.
DR GeneID; 67414756; -.
DR GeneID; 948659; -.
DR KEGG; ecj:JW5735; -.
DR KEGG; eco:b4138; -.
DR PATRIC; fig|1411691.4.peg.2562; -.
DR EchoBASE; EB1207; -.
DR eggNOG; COG2704; Bacteria.
DR HOGENOM; CLU_036056_1_1_6; -.
DR OMA; PKQINYL; -.
DR PhylomeDB; P0ABN5; -.
DR BioCyc; EcoCyc:DCUA-MON; -.
DR BioCyc; MetaCyc:DCUA-MON; -.
DR PRO; PR:P0ABN5; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; ISM:EcoCyc.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0015556; F:C4-dicarboxylate transmembrane transporter activity; IDA:EcoCyc.
DR GO; GO:0005469; F:succinate:fumarate antiporter activity; IDA:EcoCyc.
DR GO; GO:0015740; P:C4-dicarboxylate transport; IDA:EcoCyc.
DR GO; GO:0044271; P:cellular nitrogen compound biosynthetic process; IMP:EcoCyc.
DR GO; GO:0140009; P:L-aspartate import across plasma membrane; IDA:EcoCyc.
DR InterPro; IPR004668; Anaer_Dcu_memb_transpt.
DR PANTHER; PTHR36106; PTHR36106; 1.
DR Pfam; PF03605; DcuA_DcuB; 1.
DR PIRSF; PIRSF004539; C4-dicrbxl_trns; 1.
DR TIGRFAMs; TIGR00770; Dcu; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..433
FT /note="Anaerobic C4-dicarboxylate transporter DcuA"
FT /id="PRO_0000170347"
FT TRANSMEM 1..18
FT /note="Helical"
FT TOPO_DOM 19
FT /note="Cytoplasmic"
FT TRANSMEM 20..37
FT /note="Helical"
FT TOPO_DOM 38..53
FT /note="Periplasmic"
FT TRANSMEM 54..71
FT /note="Helical"
FT TOPO_DOM 72..85
FT /note="Cytoplasmic"
FT TRANSMEM 86..103
FT /note="Helical"
FT TOPO_DOM 104..132
FT /note="Periplasmic"
FT TRANSMEM 133..147
FT /note="Helical"
FT TOPO_DOM 148..228
FT /note="Cytoplasmic"
FT TRANSMEM 229..246
FT /note="Helical"
FT TOPO_DOM 247..264
FT /note="Periplasmic"
FT TRANSMEM 265..282
FT /note="Helical"
FT TOPO_DOM 283..292
FT /note="Cytoplasmic"
FT TRANSMEM 293..310
FT /note="Helical"
FT TOPO_DOM 311..332
FT /note="Periplasmic"
FT TRANSMEM 333..350
FT /note="Helical"
FT TOPO_DOM 351..355
FT /note="Cytoplasmic"
FT TRANSMEM 356..373
FT /note="Helical"
FT TOPO_DOM 374..433
FT /note="Periplasmic"
FT CONFLICT 355..356
FT /note="MP -> IA (in Ref. 6; CAA26176)"
FT /evidence="ECO:0000305"
FT CONFLICT 365..367
FT /note="PLT -> RXD (in Ref. 6)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 433 AA; 45751 MW; 049C8D186C83C9AD CRC64;
MLVVELIIVL LAIFLGARLG GIGIGFAGGL GVLVLAAIGV KPGNIPFDVI SIIMAVIAAI
SAMQVAGGLD YLVHQTEKLL RRNPKYITIL APIVTYFLTI FAGTGNISLA TLPVIAEVAK
EQGVKPCRPL STAVVSAQIA ITASPISAAV VYMSSVMEGH GISYLHLLSV VIPSTLLAVL
VMSFLVTMLF NSKLSDDPIY RKRLEEGLVE LRGEKQIEIK SGAKTSVWLF LLGVVGVVIY
AIINSPSMGL VEKPLMNTTN AILIIMLSVA TLTTVICKVD TDNILNSSTF KAGMSACICI
LGVAWLGDTF VSNNIDWIKD TAGEVIQGHP WLLAVIFFFA SALLYSQAAT AKALMPMALA
LNVSPLTAVA SFAAVSGLFI LPTYPTLVAA VQMDDTGTTR IGKFVFNHPF FIPGTLGVAL
AVCFGFVLGS FML
