DCUA_WOLSU
ID DCUA_WOLSU Reviewed; 433 AA.
AC O34245; Q56744;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 25-MAY-2022, entry version 107.
DE RecName: Full=Anaerobic C4-dicarboxylate transporter DcuA;
GN Name=dcuA; OrderedLocusNames=WS0661;
OS Wolinella succinogenes (strain ATCC 29543 / DSM 1740 / LMG 7466 / NCTC
OS 11488 / FDC 602W) (Vibrio succinogenes).
OC Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC Helicobacteraceae; Wolinella.
OX NCBI_TaxID=273121;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=11004174; DOI=10.1128/jb.182.20.5757-5764.2000;
RA Ullmann R., Gross R., Simon J., Unden G., Kroeger A.;
RT "Transport of C4-dicarboxylates in Wolinella succinogenes.";
RL J. Bacteriol. 182:5757-5764(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 29543 / DSM 1740 / CCUG 13145 / JCM 31913 / LMG 7466 / NCTC
RC 11488 / FDC 602W;
RX PubMed=14500908; DOI=10.1073/pnas.1932838100;
RA Baar C., Eppinger M., Raddatz G., Simon J., Lanz C., Klimmek O.,
RA Nandakumar R., Gross R., Rosinus A., Keller H., Jagtap P., Linke B.,
RA Meyer F., Lederer H., Schuster S.C.;
RT "Complete genome sequence and analysis of Wolinella succinogenes.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:11690-11695(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 79-433.
RX PubMed=8898907; DOI=10.1111/j.1432-1033.1996.0201t.x;
RA Lubkowski J., Palm G.J., Gilliland G.L., Derst C., Roehm K.H., Wlodawer A.;
RT "Crystal structure and amino acid sequence of Wolinella succinogenes L-
RT asparaginase.";
RL Eur. J. Biochem. 241:201-207(1996).
CC -!- FUNCTION: Responsible for the transport of C4-dicarboxylates from the
CC periplasm across the inner membrane. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the DcuA/DcuB transporter (TC 2.A.13.1) family.
CC {ECO:0000305}.
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DR EMBL; AJ002933; CAA05765.1; -; Genomic_DNA.
DR EMBL; BX571658; CAE09791.1; -; Genomic_DNA.
DR EMBL; X89215; CAA61502.1; -; Genomic_DNA.
DR PIR; S74204; S74204.
DR RefSeq; WP_011138591.1; NC_005090.1.
DR AlphaFoldDB; O34245; -.
DR STRING; 273121.WS0661; -.
DR EnsemblBacteria; CAE09791; CAE09791; WS0661.
DR KEGG; wsu:WS0661; -.
DR eggNOG; COG2704; Bacteria.
DR HOGENOM; CLU_036056_1_1_7; -.
DR OMA; PKQINYL; -.
DR OrthoDB; 591678at2; -.
DR Proteomes; UP000000422; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015556; F:C4-dicarboxylate transmembrane transporter activity; IEA:InterPro.
DR InterPro; IPR004668; Anaer_Dcu_memb_transpt.
DR PANTHER; PTHR36106; PTHR36106; 1.
DR Pfam; PF03605; DcuA_DcuB; 1.
DR PIRSF; PIRSF004539; C4-dicrbxl_trns; 1.
DR TIGRFAMs; TIGR00770; Dcu; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..433
FT /note="Anaerobic C4-dicarboxylate transporter DcuA"
FT /id="PRO_0000170354"
FT TRANSMEM 1..18
FT /note="Helical"
FT /evidence="ECO:0000250"
FT TOPO_DOM 19
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 20..38
FT /note="Helical"
FT /evidence="ECO:0000250"
FT TOPO_DOM 39..54
FT /note="Periplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 55..72
FT /note="Helical"
FT /evidence="ECO:0000250"
FT TOPO_DOM 73..86
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 87..104
FT /note="Helical"
FT /evidence="ECO:0000250"
FT TOPO_DOM 105..133
FT /note="Periplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 134..148
FT /note="Helical"
FT /evidence="ECO:0000250"
FT TOPO_DOM 149..228
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 229..246
FT /note="Helical"
FT /evidence="ECO:0000250"
FT TOPO_DOM 247..264
FT /note="Periplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 265..282
FT /note="Helical"
FT /evidence="ECO:0000250"
FT TOPO_DOM 283..292
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 293..310
FT /note="Helical"
FT /evidence="ECO:0000250"
FT TOPO_DOM 311..332
FT /note="Periplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 333..350
FT /note="Helical"
FT /evidence="ECO:0000250"
FT TOPO_DOM 351..355
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 356..373
FT /note="Helical"
FT /evidence="ECO:0000250"
FT TOPO_DOM 374..433
FT /note="Periplasmic"
FT /evidence="ECO:0000250"
FT CONFLICT 130..139
FT /note="PLSIAVVASQ -> TLEYCRGRLS (in Ref. 3; CAA61502)"
FT /evidence="ECO:0000305"
FT CONFLICT 157..160
FT /note="ALEP -> RVDR (in Ref. 3; CAA61502)"
FT /evidence="ECO:0000305"
FT CONFLICT 232
FT /note="V -> L (in Ref. 3; CAA61502)"
FT /evidence="ECO:0000305"
FT CONFLICT 286
FT /note="N -> K (in Ref. 3; CAA61502)"
FT /evidence="ECO:0000305"
FT CONFLICT 320..322
FT /note="GFA -> ALS (in Ref. 3; CAA61502)"
FT /evidence="ECO:0000305"
FT CONFLICT 336
FT /note="T -> I (in Ref. 3; CAA61502)"
FT /evidence="ECO:0000305"
FT CONFLICT 359..360
FT /note="IA -> MP (in Ref. 3; CAA61502)"
FT /evidence="ECO:0000305"
FT CONFLICT 367
FT /note="T -> S (in Ref. 3; CAA61502)"
FT /evidence="ECO:0000305"
FT CONFLICT 377
FT /note="A -> G (in Ref. 3; CAA61502)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 433 AA; 45211 MW; 8C98AE0A82A5B9DD CRC64;
MLVFEILVVL AAIFLGVRLG GIAIGYAGGL GMIVLCLGLG LKPGSIPIDV ILIIMSVIAA
IAAMQVAGGL DYLVHLAEKL LRSQPKHITF LAPVVTYFMT LLAGTGHTAF STLPVIAKVA
KEQGVKPSVP LSIAVVASQI AITASPVSAA VVFMSGALEP LGVGYLQLLA ICIPTTFIGC
MITAFICNLF NTDLSKDPVY QERVAKGMVK LRGQTQYVAK PGAKLSVLIF LVGILAVVFY
ATAISKNVGL IQNPIVGRDS AIMMFMLTTA TLIAMFAKID TDAVLNASTF KSGMTACICV
LGVAWLGDTF VSNHINDIKG FAGGILEGHP WMLAITLFFA SMLLYSQAAT AKALIPAAIA
LSVDPVTLIA SFAAVSALFV LPTYPTLLAA VQMDDTGSTR IGKFVFNHPF IVPGVLAIGI
SVALGFIVAP ILL
