DCUB_ECOLI
ID DCUB_ECOLI Reviewed; 446 AA.
AC P0ABN9; P14409; Q2M6I1;
DT 08-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 08-NOV-2005, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Anaerobic C4-dicarboxylate transporter DcuB;
GN Name=dcuB; Synonyms=genF; OrderedLocusNames=b4123, JW4084;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=8131924; DOI=10.1042/bst021342s;
RA Six S., Andrews S.C., Roberts R.E., Unden G., Guest J.R.;
RT "Construction and properties of Escherichia coli mutants defective in two
RT genes encoding homologous membrane proteins with putative roles in
RT anaerobic C4-dicarboxylic acid transport.";
RL Biochem. Soc. Trans. 21:342S-342S(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=7961398; DOI=10.1128/jb.176.21.6470-6478.1994;
RA Six S., Andrews S.C., Unden G., Guest J.R.;
RT "Escherichia coli possesses two homologous anaerobic C4-dicarboxylate
RT membrane transporters (DcuA and DcuB) distinct from the aerobic
RT dicarboxylate transport system (Dct).";
RL J. Bacteriol. 176:6470-6478(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=7610040; DOI=10.1093/nar/23.12.2105;
RA Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.;
RT "Analysis of the Escherichia coli genome VI: DNA sequence of the region
RT from 92.8 through 100 minutes.";
RL Nucleic Acids Res. 23:2105-2119(1995).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [6]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 67-446.
RC STRAIN=K12;
RX PubMed=2656658; DOI=10.1128/jb.171.6.3494-3503.1989;
RA Bell P.J., Andrews S.C., Sivak M.N., Guest J.R.;
RT "Nucleotide sequence of the FNR-regulated fumarase gene (fumB) of
RT Escherichia coli K-12.";
RL J. Bacteriol. 171:3494-3503(1989).
RN [7]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: Responsible for the transport of C4-dicarboxylates from the
CC periplasm across the inner membrane.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the DcuA/DcuB transporter (TC 2.A.13.1) family.
CC {ECO:0000305}.
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DR EMBL; X79886; CAA56258.1; -; Genomic_DNA.
DR EMBL; U14003; AAA97023.1; -; Genomic_DNA.
DR EMBL; U00096; AAC77084.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE78125.1; -; Genomic_DNA.
DR EMBL; M27058; AAA23826.1; -; Genomic_DNA.
DR PIR; S56352; S56352.
DR RefSeq; NP_418547.1; NC_000913.3.
DR RefSeq; WP_000899522.1; NZ_STEB01000014.1.
DR AlphaFoldDB; P0ABN9; -.
DR BioGRID; 4260911; 9.
DR STRING; 511145.b4123; -.
DR TCDB; 2.A.13.1.2; the c4-dicarboxylate uptake (dcu) family.
DR jPOST; P0ABN9; -.
DR PaxDb; P0ABN9; -.
DR PRIDE; P0ABN9; -.
DR EnsemblBacteria; AAC77084; AAC77084; b4123.
DR EnsemblBacteria; BAE78125; BAE78125; BAE78125.
DR GeneID; 66671966; -.
DR GeneID; 948641; -.
DR KEGG; ecj:JW4084; -.
DR KEGG; eco:b4123; -.
DR PATRIC; fig|1411691.4.peg.2577; -.
DR EchoBASE; EB0006; -.
DR eggNOG; COG2704; Bacteria.
DR HOGENOM; CLU_036056_1_1_6; -.
DR InParanoid; P0ABN9; -.
DR OMA; DKDPEFQ; -.
DR PhylomeDB; P0ABN9; -.
DR BioCyc; EcoCyc:DCUB-MON; -.
DR BioCyc; MetaCyc:DCUB-MON; -.
DR PRO; PR:P0ABN9; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; ISM:EcoCyc.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0015556; F:C4-dicarboxylate transmembrane transporter activity; IDA:EcoCyc.
DR GO; GO:0005469; F:succinate:fumarate antiporter activity; IDA:EcoCyc.
DR GO; GO:0015740; P:C4-dicarboxylate transport; IDA:EcoCyc.
DR InterPro; IPR004668; Anaer_Dcu_memb_transpt.
DR PANTHER; PTHR36106; PTHR36106; 1.
DR Pfam; PF03605; DcuA_DcuB; 1.
DR PIRSF; PIRSF004539; C4-dicrbxl_trns; 1.
DR TIGRFAMs; TIGR00770; Dcu; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..446
FT /note="Anaerobic C4-dicarboxylate transporter DcuB"
FT /id="PRO_0000170355"
FT TRANSMEM 1..19
FT /note="Helical"
FT /evidence="ECO:0000250"
FT TOPO_DOM 20
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 21..39
FT /note="Helical"
FT /evidence="ECO:0000250"
FT TOPO_DOM 40..55
FT /note="Periplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 56..73
FT /note="Helical"
FT /evidence="ECO:0000250"
FT TOPO_DOM 74..87
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 88..105
FT /note="Helical"
FT /evidence="ECO:0000250"
FT TOPO_DOM 106..134
FT /note="Periplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 135..149
FT /note="Helical"
FT /evidence="ECO:0000250"
FT TOPO_DOM 150..240
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 241..258
FT /note="Helical"
FT /evidence="ECO:0000250"
FT TOPO_DOM 259..275
FT /note="Periplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 276..293
FT /note="Helical"
FT /evidence="ECO:0000250"
FT TOPO_DOM 294..303
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 304..321
FT /note="Helical"
FT /evidence="ECO:0000250"
FT TOPO_DOM 322..343
FT /note="Periplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 344..361
FT /note="Helical"
FT /evidence="ECO:0000250"
FT TOPO_DOM 362..366
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 367..384
FT /note="Helical"
FT /evidence="ECO:0000250"
FT TOPO_DOM 385..446
FT /note="Periplasmic"
FT /evidence="ECO:0000250"
SQ SEQUENCE 446 AA; 47935 MW; B4AF89BB2E0CCB3B CRC64;
MLFTIQLIII LICLFYGARK GGIALGLLGG IGLVILVFVF HLQPGKPPVD VMLVIIAVVA
ASATLQASGG LDVMLQIAEK LLRRNPKYVS IVAPFVTCTL TILCGTGHVV YTILPIIYDV
AIKNNIRPER PMAASSIGAQ MGIIASPVSV AVVSLVAMLG NVTFDGRHLE FLDLLAITIP
STLIGILAIG IFSWFRGKDL DKDEEFQKFI SVPENREYVY GDTATLLDKK LPKSNWLAMW
IFLGAIAVVA LLGADSDLRP SFGGKPLSMV LVIQMFMLLT GALIIILTKT NPASISKNEV
FRSGMIAIVA VYGIAWMAET MFGAHMSEIQ GVLGEMVKEY PWAYAIVLLL VSKFVNSQAA
ALAAIVPVAL AIGVDPAYIV ASAPACYGYY ILPTYPSDLA AIQFDRSGTT HIGRFVINHS
FILPGLIGVS VSCVFGWIFA AMYGFL
