DCUD_ECOLI
ID DCUD_ECOLI Reviewed; 455 AA.
AC P45428; Q2M8Y6;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Putative cryptic C4-dicarboxylate transporter DcuD;
GN Name=dcuD; Synonyms=yhcL; OrderedLocusNames=b3227, JW3196;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [3]
RP FUNCTION.
RX PubMed=10525738; DOI=10.1007/s002030050763;
RA Janausch I.G., Unden G.;
RT "The dcuD (former yhcL) gene product of Escherichia coli as a member of the
RT DcuC family of C4-dicarboxylate carriers: lack of evident expression.";
RL Arch. Microbiol. 172:219-226(1999).
RN [4]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: May have a role in the transport of dicarboxylates from the
CC periplasm across the inner membrane. Seems not to be essential for
CC growth. It is likely to be a cryptic gene, as no or low expression has
CC been found under a number of growth conditions and when tested with a
CC large number of potential substrates. {ECO:0000269|PubMed:10525738}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the DcuC/DcuD transporter (TC 2.A.61) family.
CC {ECO:0000305}.
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DR EMBL; U18997; AAA58029.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76259.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77270.1; -; Genomic_DNA.
DR PIR; E65114; E65114.
DR RefSeq; NP_417694.1; NC_000913.3.
DR RefSeq; WP_000467018.1; NZ_SSZK01000034.1.
DR AlphaFoldDB; P45428; -.
DR BioGRID; 4261551; 8.
DR DIP; DIP-9412N; -.
DR IntAct; P45428; 2.
DR STRING; 511145.b3227; -.
DR TCDB; 2.A.61.1.2; the c4-dicarboxylate uptake c (dcuc) family.
DR PaxDb; P45428; -.
DR PRIDE; P45428; -.
DR EnsemblBacteria; AAC76259; AAC76259; b3227.
DR EnsemblBacteria; BAE77270; BAE77270; BAE77270.
DR GeneID; 946575; -.
DR KEGG; ecj:JW3196; -.
DR KEGG; eco:b3227; -.
DR PATRIC; fig|1411691.4.peg.3501; -.
DR EchoBASE; EB2669; -.
DR eggNOG; COG3069; Bacteria.
DR HOGENOM; CLU_030262_3_2_6; -.
DR OMA; CAIEFGP; -.
DR PhylomeDB; P45428; -.
DR BioCyc; EcoCyc:YHCL-MON; -.
DR PRO; PR:P45428; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; ISM:EcoCyc.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0015556; F:C4-dicarboxylate transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0019588; P:anaerobic glycerol catabolic process; IMP:EcoCyc.
DR GO; GO:0015740; P:C4-dicarboxylate transport; IBA:GO_Central.
DR InterPro; IPR004669; C4_dicarb_anaerob_car.
DR InterPro; IPR018385; C4_dicarb_anaerob_car-like.
DR PANTHER; PTHR42002; PTHR42002; 1.
DR Pfam; PF03606; DcuC; 1.
DR TIGRFAMs; TIGR00771; DcuC; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..455
FT /note="Putative cryptic C4-dicarboxylate transporter DcuD"
FT /id="PRO_0000201632"
FT TOPO_DOM 1..25
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 26..46
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 47..111
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 112..132
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 133
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 134..154
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 155..159
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 160..180
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 181..189
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 190..210
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 211..245
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 246..266
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 267..269
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 270..290
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 291..347
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 348..368
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 369..377
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 378..398
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 399..411
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 412..432
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 433..455
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 455 AA; 48831 MW; 4EC705EFE463FCD1 CRC64;
MFGIIISVIV LITMGYLILK NYKPQVVLAA AGIFLMMCGV WLGFGGVLDP TKSSGYLIVD
IYNEILRMLS NRIAGLGLSI MAVGGYARYM ERIGASRAMV SLLSRPLKLI RSPYIILSAT
YVIGQIMAQF ITSASGLGML LMVTLFPTLV SLGVSRLSAV AVIATTMSIE WGILETNSIF
AAQVAGMKIA TYFFHYQLPV ASCVIISVAI SHFFVQRAFD KKDKNINHEQ AEQKALDNVP
PLYYAILPVM PLILMLGSLF LAHVGLMQSE LHLVVVMLLS LTVTMFVEFF RKHNLRETMD
DVQAFFDGMG TQFANVVTLV VAGEIFAKGL TTIGTVDAVI RGAEHSGLGG IGVMIIMALV
IAICAIVMGS GNAPFMSFAS LIPNIAAGLH VPAVVMIMPM HFATTLARAV SPITAVVVVT
SGIAGVSPFA VVKRTAIPMA VGFVVNMIAT ITLFY
