DCUP_ECOLI
ID DCUP_ECOLI Reviewed; 354 AA.
AC P29680; P78135; Q2M8T4;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 3.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Uroporphyrinogen decarboxylase {ECO:0000255|HAMAP-Rule:MF_00218};
DE Short=UPD {ECO:0000255|HAMAP-Rule:MF_00218};
DE Short=URO-D {ECO:0000255|HAMAP-Rule:MF_00218};
DE EC=4.1.1.37 {ECO:0000255|HAMAP-Rule:MF_00218};
GN Name=hemE {ECO:0000255|HAMAP-Rule:MF_00218};
GN OrderedLocusNames=b3997, JW3961;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=8224882; DOI=10.1016/0378-1119(93)90233-s;
RA Nishimura K., Inokuchi H.;
RT "Cloning and sequencing of the hemE gene encoding uroporphyrinogen III
RT decarboxylase (UPD) from Escherichia coli K-12.";
RL Gene 133:109-113(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=8265357; DOI=10.1093/nar/21.23.5408;
RA Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.;
RT "Analysis of the Escherichia coli genome. IV. DNA sequence of the region
RT from 89.2 to 92.8 minutes.";
RL Nucleic Acids Res. 21:5408-5417(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
CC -!- FUNCTION: Catalyzes the decarboxylation of four acetate groups of
CC uroporphyrinogen-III to yield coproporphyrinogen-III.
CC {ECO:0000255|HAMAP-Rule:MF_00218}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=4 H(+) + uroporphyrinogen III = 4 CO2 + coproporphyrinogen
CC III; Xref=Rhea:RHEA:19865, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:57308, ChEBI:CHEBI:57309; EC=4.1.1.37;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00218};
CC -!- PATHWAY: Porphyrin-containing compound metabolism; protoporphyrin-IX
CC biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 4/4.
CC {ECO:0000255|HAMAP-Rule:MF_00218}.
CC -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00218}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00218}.
CC -!- SIMILARITY: Belongs to the uroporphyrinogen decarboxylase family.
CC {ECO:0000255|HAMAP-Rule:MF_00218}.
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DR EMBL; D12624; BAA02148.1; -; Genomic_DNA.
DR EMBL; U00006; AAC43095.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76971.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77322.1; -; Genomic_DNA.
DR PIR; H65206; H65206.
DR RefSeq; NP_418425.1; NC_000913.3.
DR RefSeq; WP_000137657.1; NZ_SSZK01000047.1.
DR AlphaFoldDB; P29680; -.
DR SMR; P29680; -.
DR BioGRID; 4263218; 2.
DR IntAct; P29680; 5.
DR STRING; 511145.b3997; -.
DR jPOST; P29680; -.
DR PaxDb; P29680; -.
DR PRIDE; P29680; -.
DR EnsemblBacteria; AAC76971; AAC76971; b3997.
DR EnsemblBacteria; BAE77322; BAE77322; BAE77322.
DR GeneID; 66672091; -.
DR GeneID; 948497; -.
DR KEGG; ecj:JW3961; -.
DR KEGG; eco:b3997; -.
DR PATRIC; fig|1411691.4.peg.2714; -.
DR EchoBASE; EB1505; -.
DR eggNOG; COG0407; Bacteria.
DR HOGENOM; CLU_040933_0_0_6; -.
DR InParanoid; P29680; -.
DR OMA; LWLMRQA; -.
DR PhylomeDB; P29680; -.
DR BioCyc; EcoCyc:UROGENDECARBOX-MON; -.
DR BioCyc; MetaCyc:UROGENDECARBOX-MON; -.
DR UniPathway; UPA00251; UER00321.
DR PRO; PR:P29680; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR GO; GO:0004853; F:uroporphyrinogen decarboxylase activity; IMP:EcoCyc.
DR GO; GO:0006785; P:heme B biosynthetic process; IMP:EcoCyc.
DR GO; GO:0006783; P:heme biosynthetic process; IMP:EcoliWiki.
DR GO; GO:0006779; P:porphyrin-containing compound biosynthetic process; IMP:EcoliWiki.
DR GO; GO:0019353; P:protoporphyrinogen IX biosynthetic process from glutamate; IMP:EcoCyc.
DR GO; GO:0006780; P:uroporphyrinogen III biosynthetic process; IMP:EcoliWiki.
DR CDD; cd00717; URO-D; 1.
DR Gene3D; 3.20.20.210; -; 1.
DR HAMAP; MF_00218; URO_D; 1.
DR InterPro; IPR038071; UROD/MetE-like_sf.
DR InterPro; IPR006361; Uroporphyrinogen_deCO2ase_HemE.
DR InterPro; IPR000257; Uroporphyrinogen_deCOase.
DR Pfam; PF01208; URO-D; 1.
DR SUPFAM; SSF51726; SSF51726; 1.
DR TIGRFAMs; TIGR01464; hemE; 1.
DR PROSITE; PS00906; UROD_1; 1.
DR PROSITE; PS00907; UROD_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Decarboxylase; Lyase; Porphyrin biosynthesis;
KW Reference proteome.
FT CHAIN 1..354
FT /note="Uroporphyrinogen decarboxylase"
FT /id="PRO_0000187603"
FT BINDING 27..31
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00218"
FT BINDING 46
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00218"
FT BINDING 77
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00218"
FT BINDING 154
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00218"
FT BINDING 209
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00218"
FT BINDING 327
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00218"
FT SITE 77
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00218"
FT CONFLICT 72..78
FT /note="AILFSDI -> RSSFRY (in Ref. 1; BAA02148)"
FT /evidence="ECO:0000305"
FT CONFLICT 83
FT /note="D -> I (in Ref. 1; BAA02148)"
FT /evidence="ECO:0000305"
FT CONFLICT 89..103
FT /note="LYFEAGEGPRFTSPV -> SSILKPEKVRVLPRQI (in Ref. 1;
FT BAA02148)"
FT /evidence="ECO:0000305"
FT CONFLICT 251..256
FT /note="GGGQWL -> SATVA (in Ref. 1; BAA02148)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 354 AA; 39248 MW; 604933C50080260C CRC64;
MTELKNDRYL RALLRQPVDV TPVWMMRQAG RYLPEYKATR AQAGDFMSLC KNAELACEVT
LQPLRRYPLD AAILFSDILT VPDAMGLGLY FEAGEGPRFT SPVTCKADVD KLPIPDPEDE
LGYVMNAVRT IRRELKGEVP LIGFSGSPWT LATYMVEGGS SKAFTVIKKM MYADPQALHA
LLDKLAKSVT LYLNAQIKAG AQAVMIFDTW GGVLTGRDYQ QFSLYYMHKI VDGLLRENDG
RRVPVTLFTK GGGQWLEAMA ETGCDALGLD WTTDIADARR RVGNKVALQG NMDPSMLYAP
PARIEEEVAT ILAGFGHGEG HVFNLGHGIH QDVPPEHAGV FVEAVHRLSE QYHR
