ID   DCUP_HORVU              Reviewed;         330 AA.
AC   Q42855;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   03-AUG-2022, entry version 99.
DE   RecName: Full=Uroporphyrinogen decarboxylase;
DE            Short=UPD;
DE            Short=URO-D;
DE            EC=4.1.1.37;
DE   Flags: Fragment;
GN   Name=DCUP;
OS   Hordeum vulgare (Barley).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX   NCBI_TaxID=4513;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Leaf;
RX   PubMed=7599310; DOI=10.1007/bf00020244;
RA   Mock H.-P., Trainotti L., Kruse E., Grimm B.;
RT   "Isolation, sequencing and expression of cDNA sequences encoding
RT   uroporphyrinogen decarboxylase from tobacco and barley.";
RL   Plant Mol. Biol. 28:245-256(1995).
CC   -!- FUNCTION: Catalyzes the decarboxylation of four acetate groups of
CC       uroporphyrinogen-III to yield coproporphyrinogen-III. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4 H(+) + uroporphyrinogen III = 4 CO2 + coproporphyrinogen
CC         III; Xref=Rhea:RHEA:19865, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:57308, ChEBI:CHEBI:57309; EC=4.1.1.37;
CC   -!- PATHWAY: Porphyrin-containing compound metabolism; protoporphyrin-IX
CC       biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 4/4.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the uroporphyrinogen decarboxylase family.
CC       {ECO:0000305}.
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DR   EMBL; X82832; CAA58039.1; -; mRNA.
DR   PIR; S55733; S55733.
DR   AlphaFoldDB; Q42855; -.
DR   SMR; Q42855; -.
DR   EnsemblPlants; HORVU.MOREX.r2.4HG0314380.1; HORVU.MOREX.r2.4HG0314380.1; HORVU.MOREX.r2.4HG0314380.
DR   EnsemblPlants; HORVU.MOREX.r2.4HG0314380.1.mrna1; HORVU.MOREX.r2.4HG0314380.1.mrna1; HORVU.MOREX.r2.4HG0314380.1.
DR   Gramene; HORVU.MOREX.r2.4HG0314380.1; HORVU.MOREX.r2.4HG0314380.1; HORVU.MOREX.r2.4HG0314380.
DR   Gramene; HORVU.MOREX.r2.4HG0314380.1.mrna1; HORVU.MOREX.r2.4HG0314380.1.mrna1; HORVU.MOREX.r2.4HG0314380.1.
DR   UniPathway; UPA00251; UER00321.
DR   ExpressionAtlas; Q42855; baseline and differential.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0004853; F:uroporphyrinogen decarboxylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0015995; P:chlorophyll biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0006782; P:protoporphyrinogen IX biosynthetic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd00717; URO-D; 1.
DR   Gene3D; 3.20.20.210; -; 1.
DR   HAMAP; MF_00218; URO_D; 1.
DR   InterPro; IPR038071; UROD/MetE-like_sf.
DR   InterPro; IPR006361; Uroporphyrinogen_deCO2ase_HemE.
DR   InterPro; IPR000257; Uroporphyrinogen_deCOase.
DR   Pfam; PF01208; URO-D; 1.
DR   SUPFAM; SSF51726; SSF51726; 1.
DR   TIGRFAMs; TIGR01464; hemE; 1.
DR   PROSITE; PS00906; UROD_1; 1.
DR   PROSITE; PS00907; UROD_2; 1.
PE   2: Evidence at transcript level;
KW   Chlorophyll biosynthesis; Chloroplast; Decarboxylase; Lyase; Plastid;
KW   Porphyrin biosynthesis.
FT   CHAIN           <1..330
FT                   /note="Uroporphyrinogen decarboxylase"
FT                   /id="PRO_0000187575"
FT   BINDING         10..14
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         29
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         59
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         60
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         137
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         192
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         307
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   SITE            60
FT                   /note="Transition state stabilizer"
FT                   /evidence="ECO:0000250"
FT   NON_TER         1
SQ   SEQUENCE   330 AA;  36667 MW;  DB2C5CA27154BC90 CRC64;
     VERPPVWLMR QAGRYMKSYQ NLCEKYPLFR ERSENVDLVV EISLQPWKVF KPDGVILFSD
     ILTPLPGMNI PFDIVKGKGP VIYDPLRTAA AVNEVREFVP EEWVPYVGQA LNLLRGEVKN
     EAAVLGFVGA PFTLASYCVE GGSSKNFSKI KRMAFAEPAI LHNLLQKFTT SMANYIKYQA
     DNGAQAVQIF DSWATELSPV DFEEFSLPYL KQIVDSVKET HPDLPLILYA SGSGGLLERL
     PLTGVDVVSL DWTVDMAEGR KRLGSNIAVQ GNVDPGVLFG SKEFITKRIY DTVQKAGSQG
     HVLNLGHGIK VGTPEENVAH FFEVAKGIRY