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DCUP_YEAST
ID   DCUP_YEAST              Reviewed;         362 AA.
AC   P32347; D6VS35;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   03-AUG-2022, entry version 182.
DE   RecName: Full=Uroporphyrinogen decarboxylase;
DE            Short=UPD;
DE            Short=URO-D;
DE            EC=4.1.1.37;
GN   Name=HEM12; Synonyms=HEM6, POP3; OrderedLocusNames=YDR047W;
GN   ORFNames=YD9609.03;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1576986; DOI=10.1111/j.1432-1033.1992.tb16868.x;
RA   Garey J.R., Labbe-Bois R., Chelstowska A., Rytka J., Harrison L.,
RA   Kushner J., Labbe P.;
RT   "Uroporphyrinogen decarboxylase in Saccharomyces cerevisiae. HEM12 gene
RT   sequence and evidence for two conserved glycines essential for enzymatic
RT   activity.";
RL   Eur. J. Biochem. 205:1011-1016(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8346678; DOI=10.1002/yea.320090608;
RA   Diflumeri C., Larocque R., Keng T.;
RT   "Molecular analysis of HEM6 (HEM12) in Saccharomyces cerevisiae, the gene
RT   for uroporphyrinogen decarboxylase.";
RL   Yeast 9:613-623(1993).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169867;
RA   Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA   Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA   Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA   Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA   Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA   Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA   Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA   Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA   Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA   Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA   Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA   Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA   Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA   Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA   Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA   Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA   Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA   Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA   Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA   Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA   Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA   Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA   Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA   Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA   Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA   Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA   Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA   Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA   Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA   Mewes H.-W., Zollner A., Zaccaria P.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL   Nature 387:75-78(1997).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [5]
RP   MUTANTS.
RX   PubMed=1471989; DOI=10.1042/bj2880753;
RA   Chelstowska A., Zoadek T., Garey J.R., Kushner J., Rytka J., Labbe-Bois R.;
RT   "Identification of amino acid changes affecting yeast uroporphyrinogen
RT   decarboxylase activity by sequence analysis of hem12 mutant alleles.";
RL   Biochem. J. 288:753-757(1992).
RN   [6]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [7]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [8]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA   Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA   Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA   Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT   "N-terminal acetylome analyses and functional insights of the N-terminal
RT   acetyltransferase NatB.";
RL   Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC   -!- FUNCTION: Catalyzes the decarboxylation of four acetate groups of
CC       uroporphyrinogen-III to yield coproporphyrinogen-III. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=4 H(+) + uroporphyrinogen III = 4 CO2 + coproporphyrinogen
CC         III; Xref=Rhea:RHEA:19865, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:57308, ChEBI:CHEBI:57309; EC=4.1.1.37;
CC   -!- PATHWAY: Porphyrin-containing compound metabolism; protoporphyrin-IX
CC       biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 4/4.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- INTERACTION:
CC       P32347; P39940: RSP5; NbExp=2; IntAct=EBI-5711, EBI-16219;
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:14562095}. Cytoplasm
CC       {ECO:0000269|PubMed:14562095}.
CC   -!- MISCELLANEOUS: Present with 9220 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the uroporphyrinogen decarboxylase family.
CC       {ECO:0000305}.
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DR   EMBL; X63721; CAA45253.1; -; Genomic_DNA.
DR   EMBL; Z19089; CAA79514.1; -; Genomic_DNA.
DR   EMBL; Z49209; CAA89078.1; -; Genomic_DNA.
DR   EMBL; BK006938; DAA11895.1; -; Genomic_DNA.
DR   PIR; S23471; S23471.
DR   RefSeq; NP_010332.3; NM_001180355.3.
DR   AlphaFoldDB; P32347; -.
DR   SMR; P32347; -.
DR   BioGRID; 32102; 152.
DR   IntAct; P32347; 3.
DR   MINT; P32347; -.
DR   STRING; 4932.YDR047W; -.
DR   MaxQB; P32347; -.
DR   PaxDb; P32347; -.
DR   PRIDE; P32347; -.
DR   EnsemblFungi; YDR047W_mRNA; YDR047W; YDR047W.
DR   GeneID; 851617; -.
DR   KEGG; sce:YDR047W; -.
DR   SGD; S000002454; HEM12.
DR   VEuPathDB; FungiDB:YDR047W; -.
DR   eggNOG; KOG2872; Eukaryota.
DR   GeneTree; ENSGT00390000018302; -.
DR   HOGENOM; CLU_040933_0_0_1; -.
DR   InParanoid; P32347; -.
DR   OMA; LWLMRQA; -.
DR   BioCyc; YEAST:YDR047W-MON; -.
DR   BRENDA; 4.1.1.37; 984.
DR   Reactome; R-SCE-189451; Heme biosynthesis.
DR   UniPathway; UPA00251; UER00321.
DR   PRO; PR:P32347; -.
DR   Proteomes; UP000002311; Chromosome IV.
DR   RNAct; P32347; protein.
DR   GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; HDA:SGD.
DR   GO; GO:0004853; F:uroporphyrinogen decarboxylase activity; IDA:SGD.
DR   GO; GO:0006783; P:heme biosynthetic process; IMP:SGD.
DR   GO; GO:0006782; P:protoporphyrinogen IX biosynthetic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd00717; URO-D; 1.
DR   Gene3D; 3.20.20.210; -; 1.
DR   HAMAP; MF_00218; URO_D; 1.
DR   InterPro; IPR038071; UROD/MetE-like_sf.
DR   InterPro; IPR006361; Uroporphyrinogen_deCO2ase_HemE.
DR   InterPro; IPR000257; Uroporphyrinogen_deCOase.
DR   Pfam; PF01208; URO-D; 1.
DR   SUPFAM; SSF51726; SSF51726; 1.
DR   TIGRFAMs; TIGR01464; hemE; 1.
DR   PROSITE; PS00906; UROD_1; 1.
DR   PROSITE; PS00907; UROD_2; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Decarboxylase; Heme biosynthesis; Lyase; Nucleus;
KW   Porphyrin biosynthesis; Reference proteome.
FT   CHAIN           1..362
FT                   /note="Uroporphyrinogen decarboxylase"
FT                   /id="PRO_0000187577"
FT   BINDING         30..34
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         48
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         80
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         81
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         160
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         215
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         338
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   SITE            81
FT                   /note="Transition state stabilizer"
FT                   /evidence="ECO:0000250"
FT   VARIANT         59
FT                   /note="S -> F (in HEM12-6 and HEM12-12)"
FT   VARIANT         62
FT                   /note="T -> I (in HEM12-14)"
FT   VARIANT         107
FT                   /note="L -> S (in HEM12-3 and HEM12-13)"
FT   VARIANT         215
FT                   /note="S -> N (in HEM12-2 and HEM12-11)"
FT   MUTAGEN         33
FT                   /note="G->D: Inactivation."
FT   MUTAGEN         300
FT                   /note="G->D: Inactivation."
SQ   SEQUENCE   362 AA;  41349 MW;  E9CB3A48E62BC277 CRC64;
     MGNFPAPKND LILRAAKGEK VERPPCWIMR QAGRYLPEYH EVKNNRDFFQ TCRDAEIASE
     ITIQPVRRYR GLIDAAIIFS DILVIPQAMG MRVEMLEGKG PHFPEPLRNP EDLQTVLDYK
     VDVLKELDWA FKAITMTRIK LDGEVPLFGF CGGPWTLMVY MTEGGGSRLF RFAKQWINMY
     PELSHKLLQK ITDVAVEFLS QQVVAGAQIL QVFESWGGEL SSVDFDEFSL PYLRQIAERV
     PKRLQELGIM EQIPMIVFAK GSWYALDKLC CSGFDVVSLD WSWDPREAVK INKNRVTLQG
     NLDPGVMYGS KEVITKKVKQ MIEAFGGGKS RYIVNFGHGT HPFMDPDVIK FFLEECHRIG
     SK
 
 
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