ID   DCVR_CUCSA              Reviewed;         419 AA.
AC   J9RYI6;
DT   29-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT   28-NOV-2012, sequence version 1.
DT   25-MAY-2022, entry version 30.
DE   RecName: Full=Divinyl chlorophyllide a 8-vinyl-reductase, chloroplastic;
DE            EC=1.3.1.75 {ECO:0000269|PubMed:23154534};
DE   Flags: Precursor;
GN   Name=DVR;
OS   Cucumis sativus (Cucumber).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Cucurbitales; Cucurbitaceae; Benincaseae; Cucumis.
OX   NCBI_TaxID=3659;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, AND
RP   BIOPHYSICOCHEMICAL PROPERTIES.
RX   PubMed=23154534; DOI=10.1104/pp.112.208421;
RA   Wang P., Wan C., Xu Z., Wang P., Wang W., Sun C., Ma X., Xiao Y., Zhu J.,
RA   Gao X., Deng X.;
RT   "One divinyl reductase reduces the 8-vinyl groups in various intermediates
RT   of chlorophyll biosynthesis in a given higher plant species, but the
RT   isozyme differs between species.";
RL   Plant Physiol. 161:521-534(2013).
CC   -!- FUNCTION: Catalyzes the conversion of divinyl chlorophyllide to
CC       monovinyl chlorophyllide. Reduces the 8-vinyl group of the tetrapyrrole
CC       to an ethyl group using NADPH as the reductant. The best substrate is
CC       (3,8-divinyl)-chlorophyllide a (DV-Chlidea). Very low activity with
CC       (3,8-divinyl)-protochlorophyllide a (DV-Pchlidea) and (3,8-divinyl)-
CC       magnesium-protoporphyrin IX monomethyl ester (DV-MPE). No activity with
CC       (3,8-divinyl)-magnesium-protoporphyrin IX (DV-Mg-Proto) and (3,8-
CC       divinyl)-chlorophyll a (DV-Chla). {ECO:0000269|PubMed:23154534}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=NADP(+) + protochlorophyllide a = 3,8-divinyl
CC         protochlorophyllide a + H(+) + NADPH; Xref=Rhea:RHEA:48884,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349,
CC         ChEBI:CHEBI:58632, ChEBI:CHEBI:83350; EC=1.3.1.75;
CC         Evidence={ECO:0000269|PubMed:23154534};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Optimum pH is 7.0 with DV-Chlidea or DV-Pchlidea as substrate,
CC         optimum pH is 6.5 with DV-MPE as substrate.
CC         {ECO:0000269|PubMed:23154534};
CC       Temperature dependence:
CC         Optimum temperature is 25 degrees Celsius with DV-Chlidea as
CC         substrate, Optimum temperature is 20 degrees Celsius with DV-Pchlidea
CC         or DV-MPE as substrate. {ECO:0000269|PubMed:23154534};
CC   -!- PATHWAY: Porphyrin-containing compound metabolism; chlorophyll
CC       biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000250}.
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DR   EMBL; JX239753; AFR53113.1; -; Genomic_DNA.
DR   RefSeq; XP_004134186.1; XM_004134138.2.
DR   AlphaFoldDB; J9RYI6; -.
DR   SMR; J9RYI6; -.
DR   STRING; 3659.XP_004159826.1; -.
DR   EnsemblPlants; KGN57068; KGN57068; Csa_3G151460.
DR   GeneID; 101218189; -.
DR   Gramene; KGN57068; KGN57068; Csa_3G151460.
DR   KEGG; csv:101218189; -.
DR   eggNOG; KOG1203; Eukaryota.
DR   OMA; FARIGKY; -.
DR   BRENDA; 1.3.1.75; 1733.
DR   UniPathway; UPA00668; -.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0051744; F:3,8-divinyl protochlorophyllide a 8-vinyl reductase activity; IEA:RHEA.
DR   GO; GO:0033728; F:divinyl chlorophyllide a 8-vinyl-reductase activity; IEA:UniProtKB-EC.
DR   GO; GO:0015995; P:chlorophyll biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR044201; DVR-like.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   PANTHER; PTHR47378; PTHR47378; 1.
DR   Pfam; PF13460; NAD_binding_10; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   1: Evidence at protein level;
KW   Chlorophyll biosynthesis; Chloroplast; NADP; Oxidoreductase; Plastid;
KW   Transit peptide.
FT   TRANSIT         1..71
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           72..419
FT                   /note="Divinyl chlorophyllide a 8-vinyl-reductase,
FT                   chloroplastic"
FT                   /id="PRO_0000422538"
SQ   SEQUENCE   419 AA;  45586 MW;  AC57388CB0197C41 CRC64;
     MSICSTVGAG LNLHSPANAT NSTRLSSNFV HQIPVSSFSF SFQSSSLRLS QTPKFSRQRR
     NPIVVSSTPV VESTKSSFRA KNPKDTNILV VGSTGYIGNF VVKELVSRGF NVIAIAREKS
     GIKGRNSKEQ ASDQLKGANV CFSDVSHLDV LEKSLGDLDV PIDVVVSCLA SRTGGIKDSW
     KIDYEATKNS LVAGRNRGAS HFVLLSAICV QKPLLEFQRA KLKFEAELME AAKEDSGFTY
     SIVRPTAFFK SLGGQVELVK DGKPYVMFGD GKLCACKPIS EQDLASFIAD CVLSEDKINQ
     VLPIGGPGKA LTPLEQGEIL FRLLGKEPNF FKVPIGIMDF AIGVLDFLVK FFPAMEDAAE
     YGKIGRYYAA ESMLILDPET GEYSADKTPS YGKDTLEDFF ERVLSEGMAG QELGEQSVF