DCVR_ORYSI
ID DCVR_ORYSI Reviewed; 405 AA.
AC D5L1S4; B8APG8;
DT 29-MAY-2013, integrated into UniProtKB/Swiss-Prot.
DT 15-JUN-2010, sequence version 1.
DT 25-MAY-2022, entry version 63.
DE RecName: Full=Divinyl chlorophyllide a 8-vinyl-reductase, chloroplastic;
DE EC=1.3.1.75 {ECO:0000269|PubMed:20484022, ECO:0000269|PubMed:23154534};
DE Flags: Precursor;
GN Name=DVR; ORFNames=OsI_11570;
OS Oryza sativa subsp. indica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39946;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, AND
RP MUTAGENESIS OF 318-LYS--PRO-320.
RX PubMed=20484022; DOI=10.1104/pp.110.158477;
RA Wang P., Gao J., Wan C., Zhang F., Xu Z., Huang X., Sun X., Deng X.;
RT "Divinyl chlorophyll(ide) a can be converted to monovinyl chlorophyll(ide)
RT a by a divinyl reductase in rice.";
RL Plant Physiol. 153:994-1003(2010).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. 93-11;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [3]
RP FUNCTION, CATALYTIC ACTIVITY, AND BIOPHYSICOCHEMICAL PROPERTIES.
RX PubMed=23154534; DOI=10.1104/pp.112.208421;
RA Wang P., Wan C., Xu Z., Wang P., Wang W., Sun C., Ma X., Xiao Y., Zhu J.,
RA Gao X., Deng X.;
RT "One divinyl reductase reduces the 8-vinyl groups in various intermediates
RT of chlorophyll biosynthesis in a given higher plant species, but the
RT isozyme differs between species.";
RL Plant Physiol. 161:521-534(2013).
CC -!- FUNCTION: Catalyzes the conversion of divinyl chlorophyllide to
CC monovinyl chlorophyllide. Reduces the 8-vinyl group of the tetrapyrrole
CC to an ethyl group using NADPH as the reductant. Can use (3,8-divinyl)-
CC chlorophyllide a (DV-Chlidea) > (3,8-divinyl)-chlorophyll a (DV-Chla) >
CC (3,8-divinyl)-protochlorophyllide a (DV-Pchlidea) > (3,8-divinyl)-
CC magnesium-protoporphyrin IX monomethyl ester (DV-MPE) > (3,8-divinyl)-
CC magnesium-protoporphyrin IX (DV-Mg-Proto) as substrates.
CC {ECO:0000269|PubMed:20484022, ECO:0000269|PubMed:23154534}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=NADP(+) + protochlorophyllide a = 3,8-divinyl
CC protochlorophyllide a + H(+) + NADPH; Xref=Rhea:RHEA:48884,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349,
CC ChEBI:CHEBI:58632, ChEBI:CHEBI:83350; EC=1.3.1.75;
CC Evidence={ECO:0000269|PubMed:20484022, ECO:0000269|PubMed:23154534};
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC pH dependence:
CC Optimum pH is 7.0 with DV-Chlidea or DV-Pchlidea as substrate,
CC optimum pH is 6.5 with DV-Chla, DV-MPE or DV-Mg-Proto as substrate.
CC {ECO:0000269|PubMed:23154534};
CC Temperature dependence:
CC Optimum temperature is 25 degrees Celsius with DV-Chlidea or DV-Chla
CC as substrate, Optimum temperature is 20 degrees Celsius with DV-
CC Pchlidea, DV-MPE or DV-Mg-Proto as substrate.
CC {ECO:0000269|PubMed:23154534};
CC -!- PATHWAY: Porphyrin-containing compound metabolism; chlorophyll
CC biosynthesis.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000250}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EEC75257.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; GU733918; ADE43128.1; -; Genomic_DNA.
DR EMBL; CM000128; EEC75257.1; ALT_INIT; Genomic_DNA.
DR AlphaFoldDB; D5L1S4; -.
DR SMR; D5L1S4; -.
DR STRING; 39946.D5L1S4; -.
DR HOGENOM; CLU_1716135_0_0_1; -.
DR BRENDA; 1.3.1.75; 4460.
DR UniPathway; UPA00668; -.
DR Proteomes; UP000007015; Chromosome 3.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0051744; F:3,8-divinyl protochlorophyllide a 8-vinyl reductase activity; IEA:RHEA.
DR GO; GO:0033728; F:divinyl chlorophyllide a 8-vinyl-reductase activity; IEA:UniProtKB-EC.
DR GO; GO:0015995; P:chlorophyll biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR044201; DVR-like.
DR InterPro; IPR016040; NAD(P)-bd_dom.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR PANTHER; PTHR47378; PTHR47378; 1.
DR Pfam; PF13460; NAD_binding_10; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 1: Evidence at protein level;
KW Chlorophyll biosynthesis; Chloroplast; NADP; Oxidoreductase; Plastid;
KW Reference proteome; Transit peptide.
FT TRANSIT 1..58
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 59..405
FT /note="Divinyl chlorophyllide a 8-vinyl-reductase,
FT chloroplastic"
FT /id="PRO_0000422536"
FT MUTAGEN 318..320
FT /note="Missing: Loss of catalytic activity and exclusive
FT accumulation of divinyl chlorophylls."
FT /evidence="ECO:0000269|PubMed:20484022"
SQ SEQUENCE 405 AA; 43267 MW; C147C8A19616D562 CRC64;
MAALLLSSHL TAASSSSTTS PTARPAPSFV SFRAANAAPK GARRGWPFLA SSVEPPPAAS
AAQPFRSLAP SETTVLVTGA TGYIGRYVVR ELLRRGHPVV AVARPRSGLR GRNGPDEVVA
DLAPARVVFS DVTDAGALRA DLSPHGPIHA AVCCLASRGG GVRDSWRVDY RATLHTLQAA
RGLGAAHFVL LSAVCVQKPL LEFQRAKLRF EGELAAEASR DPSFTYSIVR PTAFFKSLGG
QVETVKNGQP YVMFGDGKLC ACKPISEEDL AAFIADCISD EGKANKILPI GGPGKALTPL
EQGEMLFRLL GREPRFIKVP IQVMDAAIWV LDALAKVFPG VEDAAEFGKI GRYYASESML
VLDPDTGEYS DEMTPSYGSD TLEQFFERVI REGMAGQELG EQTIF
