ID   DD31_DICDI              Reviewed;         269 AA.
AC   Q02465; Q54I02;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=Prespore protein Dd31;
GN   Name=spiA; ORFNames=DDB_G0289075;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=2010032; DOI=10.1016/0012-1606(91)90421-x;
RA   Richardson D.L., Hong C.B., Loomis W.F.;
RT   "A prespore gene, Dd31, expressed during culmination of Dictyostelium
RT   discoideum.";
RL   Dev. Biol. 144:269-280(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [3]
RP   CHARACTERIZATION.
RX   PubMed=1592257; DOI=10.1101/gad.6.6.1058;
RA   Richardson D.L., Loomis W.F.;
RT   "Disruption of the sporulation-specific gene spiA in Dictyostelium
RT   discoideum leads to spore instability.";
RL   Genes Dev. 6:1058-1070(1992).
RN   [4]
RP   DEVELOPMENTAL STAGE, AND INDUCTION [LARGE SCALE ANALYSIS].
RX   PubMed=17950724; DOI=10.1016/j.ydbio.2007.08.055;
RA   Prabhu Y., Mondal S., Eichinger L., Noegel A.A.;
RT   "A GPCR involved in post aggregation events in Dictyostelium discoideum.";
RL   Dev. Biol. 312:29-43(2007).
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}. Spore coat. Note=Associated with the inner face
CC       of spore coat.
CC   -!- DEVELOPMENTAL STAGE: Expressed late in development and immediately
CC       prior to spore formation and in spores during culmination.
CC       {ECO:0000269|PubMed:17950724}.
CC   -!- INDUCTION: Induced by srfA, during development. Down-regulated in grlA
CC       null-cells at 16 hours of starvation. {ECO:0000269|PubMed:17950724}.
CC   -!- SIMILARITY: Belongs to the SCAMP family. {ECO:0000305}.
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DR   EMBL; X54452; CAA38320.1; -; Genomic_DNA.
DR   EMBL; AAFI02000130; EAL62887.1; -; Genomic_DNA.
DR   PIR; S16671; S16671.
DR   RefSeq; XP_636403.1; XM_631311.1.
DR   AlphaFoldDB; Q02465; -.
DR   SMR; Q02465; -.
DR   STRING; 44689.DDB0191438; -.
DR   PaxDb; Q02465; -.
DR   EnsemblProtists; EAL62887; EAL62887; DDB_G0289075.
DR   GeneID; 8626962; -.
DR   KEGG; ddi:DDB_G0289075; -.
DR   dictyBase; DDB_G0289075; spiA.
DR   eggNOG; ENOG502RBVX; Eukaryota.
DR   HOGENOM; CLU_1035987_0_0_1; -.
DR   InParanoid; Q02465; -.
DR   OMA; SHFEKEQ; -.
DR   PhylomeDB; Q02465; -.
DR   Reactome; R-DDI-6798695; Neutrophil degranulation.
DR   PRO; PR:Q02465; -.
DR   Proteomes; UP000002195; Chromosome 5.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0055038; C:recycling endosome membrane; IBA:GO_Central.
DR   GO; GO:0031160; C:spore wall; IDA:dictyBase.
DR   GO; GO:0032588; C:trans-Golgi network membrane; IBA:GO_Central.
DR   GO; GO:0031154; P:culmination involved in sorocarp development; IEP:dictyBase.
DR   GO; GO:0015031; P:protein transport; IBA:GO_Central.
DR   GO; GO:0030587; P:sorocarp development; IMP:dictyBase.
DR   InterPro; IPR007273; SCAMP.
DR   PANTHER; PTHR10687; PTHR10687; 1.
DR   Pfam; PF04144; SCAMP; 1.
PE   1: Evidence at protein level;
KW   Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..269
FT                   /note="Prespore protein Dd31"
FT                   /id="PRO_0000079836"
FT   TRANSMEM        111..131
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        139..159
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        177..197
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        225..245
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          1..35
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   269 AA;  30620 MW;  0A1AEDC5D1FE05AA CRC64;
     MEHNNNPGTP QMSSEFPAST TQTSSSAAAY DNSSHFEKEQ SLMRWEQDLK LRERALANNQ
     SAADITGPHV VVPAPATAHP RQANFPSSYP MIRLNLEEDI AIREYRQIVK FGIFVFLWEA
     AALVYNWVVS IGTIVYSAVD NFFLALFYMI VGVPTLYFLT RKLYRAASVP ERARKSYAYL
     MALLGVVLFN IIFFVGFKRS GMNGLIWVIS LFHNDHNAVG AMATVSLFFW FVGVFLTIAL
     FIMYLRLNNT KRQRGEIQNA GFREYIKSR