ID   DD3_DICDI               Reviewed;         616 AA.
AC   Q58A42; Q54RK9;
DT   20-APR-2010, integrated into UniProtKB/Swiss-Prot.
DT   10-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 61.
DE   RecName: Full=Protein DD3-3;
DE   Flags: Precursor;
GN   Name=DD3-3; ORFNames=DDB_G0283095;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, AND
RP   DEVELOPMENTAL STAGE.
RC   STRAIN=AX2;
RX   PubMed=15883003; DOI=10.1016/j.bbrc.2005.03.244;
RA   Sakuragi N., Ogasawara N., Tanesaka E., Yoshida M.;
RT   "Functional analysis of a novel gene, DD3-3, from Dictyostelium
RT   discoideum.";
RL   Biochem. Biophys. Res. Commun. 331:1201-1206(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [3]
RP   INDUCTION [LARGE SCALE ANALYSIS].
RX   PubMed=18559084; DOI=10.1186/1471-2164-9-291;
RA   Sillo A., Bloomfield G., Balest A., Balbo A., Pergolizzi B., Peracino B.,
RA   Skelton J., Ivens A., Bozzaro S.;
RT   "Genome-wide transcriptional changes induced by phagocytosis or growth on
RT   bacteria in Dictyostelium.";
RL   BMC Genomics 9:291-291(2008).
CC   -!- FUNCTION: May participate in O-glycosylation.
CC       {ECO:0000269|PubMed:15883003}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type I
CC       membrane protein {ECO:0000305}.
CC   -!- DEVELOPMENTAL STAGE: Expression appears at the growth phase stage and
CC       rapidly increases after starvation began. It reaches its peak at 3
CC       hours and is continually expressed for 12 hours after starvation has
CC       begun. {ECO:0000269|PubMed:15883003}.
CC   -!- INDUCTION: Down-regulated by phagocytic stimuli.
CC       {ECO:0000269|PubMed:18559084}.
CC   -!- DISRUPTION PHENOTYPE: Displays down-regulation of regA, cAMP-dependent
CC       phosphodiesterase, and overexpression of protein tyrosine kinase during
CC       early development and its shutdown during late development.
CC       {ECO:0000269|PubMed:15883003}.
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DR   EMBL; AB120173; BAD93186.1; -; mRNA.
DR   EMBL; AAFI02000050; EAL65877.1; -; Genomic_DNA.
DR   RefSeq; XP_639227.1; XM_634135.1.
DR   AlphaFoldDB; Q58A42; -.
DR   BioGRID; 1248916; 1.
DR   STRING; 44689.DDB0231683; -.
DR   PaxDb; Q58A42; -.
DR   EnsemblProtists; EAL65877; EAL65877; DDB_G0283095.
DR   GeneID; 8623913; -.
DR   KEGG; ddi:DDB_G0283095; -.
DR   dictyBase; DDB_G0283095; DD3-3.
DR   eggNOG; ENOG502QRWS; Eukaryota.
DR   HOGENOM; CLU_020767_1_0_1; -.
DR   InParanoid; Q58A42; -.
DR   OMA; YYPYWAP; -.
DR   PhylomeDB; Q58A42; -.
DR   PRO; PR:Q58A42; -.
DR   Proteomes; UP000002195; Chromosome 4.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Membrane; Reference proteome; Signal; Transmembrane;
KW   Transmembrane helix.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..616
FT                   /note="Protein DD3-3"
FT                   /id="PRO_0000393565"
FT   TOPO_DOM        22..563
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        564..584
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        585..616
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   REGION          27..55
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        148
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        291
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        317
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        355
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        550
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   616 AA;  69498 MW;  2E001672576E6DB8 CRC64;
     MRFLSSLIIL LIITIYSSVN GDIYMHSPPG SNNRNQEQGQ NRDNNQRLFN SQNNDKGGYC
     RGEALEWYEK SYLPVEWTNQ HGCGSGETEC NIVIQYMCTD NAKAADTEYI RDGTVTTTIS
     ANDITQSSTK ETNEYKFGMH ESYHHYQNCT GRERNKGLFI ANQNIGNNAD RTRQSNNGNN
     YGYECQEERD YYPYWAPSPW KDVAIITDNR DLCKWYRSES QNVQPKYYCK AATTNNNQPR
     SIDPVICANQ GGVWTKVDSH DIGAPECIKA EWTQVNTLGH VLEDGHNPMY NWSLPHSGME
     PCIATGTCSC VLRVRYNISS SDLKGNGDEF TDYKSNGPKG ATNVNDPNVV VAGQNVTLNI
     NPNQYGRTFQ DRSHVFSIIA RPDSLKKAKI WNLLVKGKRG NIVQSHPAQE YQFVPKTLHI
     KVNEYIHYQW TGCDFNPQNY DGTGIKGSDR SNMCQIKTLN HNYPMTDDEL NDDNALFADM
     ETRLRFARLG QKSCLTYDEL MTKNNNNKDA VEVDPQNCMV LNNAGTRFNG GVQKMSKTGS
     YYYMSTRNNN FSNRNQKGAI HVDPLLAGWQ IGLIASGCSL FVIISATAGC VTFARFHPHS
     GMHRAVSKVP GLKRLV