DDAH2_BOVIN
ID DDAH2_BOVIN Reviewed; 285 AA.
AC Q3SX44;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=N(G),N(G)-dimethylarginine dimethylaminohydrolase 2;
DE Short=DDAH-2;
DE Short=Dimethylarginine dimethylaminohydrolase 2;
DE EC=3.5.3.18;
DE AltName: Full=DDAHII;
DE AltName: Full=Dimethylargininase-2;
GN Name=DDAH2;
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Hereford; TISSUE=Uterus;
RG NIH - Mammalian Gene Collection (MGC) project;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-
CC monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has
CC therefore a role in the regulation of nitric oxide generation (By
CC similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + N(omega),N(omega)-dimethyl-L-arginine = dimethylamine +
CC L-citrulline; Xref=Rhea:RHEA:17305, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:57743, ChEBI:CHEBI:58040, ChEBI:CHEBI:58326; EC=3.5.3.18;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Mitochondrion
CC {ECO:0000250}. Note=Translocates from cytosol to mitochondrion upon IL-
CC 1beta stimulation in chondrocytes. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DDAH family. {ECO:0000305}.
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DR EMBL; BC104509; AAI04510.1; -; mRNA.
DR RefSeq; NP_001029876.1; NM_001034704.1.
DR RefSeq; XP_005223736.1; XM_005223679.3.
DR AlphaFoldDB; Q3SX44; -.
DR SMR; Q3SX44; -.
DR STRING; 9913.ENSBTAP00000017991; -.
DR PaxDb; Q3SX44; -.
DR PeptideAtlas; Q3SX44; -.
DR PRIDE; Q3SX44; -.
DR Ensembl; ENSBTAT00000017991; ENSBTAP00000017991; ENSBTAG00000013530.
DR Ensembl; ENSBTAT00000085909; ENSBTAP00000066281; ENSBTAG00000013530.
DR GeneID; 540386; -.
DR KEGG; bta:540386; -.
DR CTD; 23564; -.
DR VEuPathDB; HostDB:ENSBTAG00000013530; -.
DR VGNC; VGNC:27940; DDAH2.
DR eggNOG; ENOG502QW4J; Eukaryota.
DR GeneTree; ENSGT00940000160769; -.
DR HOGENOM; CLU_067923_0_0_1; -.
DR InParanoid; Q3SX44; -.
DR OMA; CPYGRFT; -.
DR OrthoDB; 1469762at2759; -.
DR TreeFam; TF314737; -.
DR BRENDA; 3.5.3.18; 908.
DR Reactome; R-BTA-203615; eNOS activation.
DR Proteomes; UP000009136; Chromosome 23.
DR Bgee; ENSBTAG00000013530; Expressed in cardiac atrium and 107 other tissues.
DR GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR GO; GO:0016597; F:amino acid binding; IBA:GO_Central.
DR GO; GO:0016403; F:dimethylargininase activity; ISS:UniProtKB.
DR GO; GO:0006527; P:arginine catabolic process; IEA:InterPro.
DR GO; GO:0006525; P:arginine metabolic process; IBA:GO_Central.
DR GO; GO:0000052; P:citrulline metabolic process; ISS:UniProtKB.
DR GO; GO:0045429; P:positive regulation of nitric oxide biosynthetic process; IDA:BHF-UCL.
DR InterPro; IPR033199; DDAH.
DR InterPro; IPR033202; DDAH2.
DR PANTHER; PTHR12737; PTHR12737; 1.
DR PANTHER; PTHR12737:SF16; PTHR12737:SF16; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Hydrolase; Mitochondrion; Reference proteome.
FT CHAIN 1..285
FT /note="N(G),N(G)-dimethylarginine dimethylaminohydrolase 2"
FT /id="PRO_0000270759"
FT ACT_SITE 171
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT ACT_SITE 276
FT /note="Nucleophile"
FT /evidence="ECO:0000255"
SQ SEQUENCE 285 AA; 29780 MW; 663378431BEF4152 CRC64;
MGTPGEGLGR CSHALIRGVP ESLASGEGAA AGLPALDLAK AQREHGVLGG KLRQRLGLQL
VELPPEESLP LGPLLGDTAV IQGDTALITR PWSPARRPEV DGVRKALQDL GLRIVEMGDE
NATLDGTDVL FTGREFFVGL SKWTNHRGAE IVADTFRDFA VSTVPVTSTS HLRGLCGMGG
PRTVVAGSSE AAQKAVRAMA VLTDHPYASL TLPDDAAADC LFLRPGQPGL PPFLLHRGGG
DLPNSQEALQ KLSDVTLVPV SCSELEKAGA GLSSLCLVLS TRPHN
