ID   DDAH_MYCTU              Reviewed;         302 AA.
AC   P71889; F2GIS4; I6YD48; Q7D7B6;
DT   25-APR-2018, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   03-AUG-2022, entry version 128.
DE   RecName: Full=N(G),N(G)-dimethylarginine dimethylaminohydrolase {ECO:0000305};
DE            Short=DDAH {ECO:0000303|PubMed:10510241};
DE            Short=Dimethylarginine dimethylaminohydrolase {ECO:0000303|PubMed:10510241};
DE            EC=3.5.3.18 {ECO:0000269|PubMed:10510241};
DE   AltName: Full=Dimethylargininase {ECO:0000305};
GN   OrderedLocusNames=Rv2323c {ECO:0000312|EMBL:CCP45110.1};
OS   Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83332;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 25618 / H37Rv;
RX   PubMed=9634230; DOI=10.1038/31159;
RA   Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA   Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA   Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA   Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA   Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA   Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA   Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA   Barrell B.G.;
RT   "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT   genome sequence.";
RL   Nature 393:537-544(1998).
RN   [2]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=10510241; DOI=10.1046/j.1365-2958.1999.01580.x;
RA   Santa Maria J., Vallance P., Charles I.G., Leiper J.M.;
RT   "Identification of microbial dimethylarginine dimethylaminohydrolase
RT   enzymes.";
RL   Mol. Microbiol. 33:1278-1279(1999).
CC   -!- FUNCTION: Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-
CC       monomethyl-L-arginine (MMA). {ECO:0000269|PubMed:10510241}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + N(omega),N(omega)-dimethyl-L-arginine = dimethylamine +
CC         L-citrulline; Xref=Rhea:RHEA:17305, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:57743, ChEBI:CHEBI:58040, ChEBI:CHEBI:58326; EC=3.5.3.18;
CC         Evidence={ECO:0000269|PubMed:10510241};
CC   -!- SIMILARITY: Belongs to the DDAH family. {ECO:0000305}.
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DR   EMBL; AL123456; CCP45110.1; -; Genomic_DNA.
DR   RefSeq; NP_216839.1; NC_000962.3.
DR   RefSeq; WP_003411960.1; NC_000962.3.
DR   AlphaFoldDB; P71889; -.
DR   SMR; P71889; -.
DR   STRING; 83332.Rv2323c; -.
DR   PaxDb; P71889; -.
DR   DNASU; 885480; -.
DR   GeneID; 885480; -.
DR   KEGG; mtu:Rv2323c; -.
DR   PATRIC; fig|83332.111.peg.2587; -.
DR   TubercuList; Rv2323c; -.
DR   eggNOG; COG1834; Bacteria.
DR   OMA; GAKCCTL; -.
DR   PhylomeDB; P71889; -.
DR   Proteomes; UP000001584; Chromosome.
DR   GO; GO:0016403; F:dimethylargininase activity; IDA:MTBBASE.
PE   1: Evidence at protein level;
KW   Hydrolase; Reference proteome.
FT   CHAIN           1..302
FT                   /note="N(G),N(G)-dimethylarginine dimethylaminohydrolase"
FT                   /id="PRO_0000443949"
FT   ACT_SITE        201
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:Q9I4E3"
FT   ACT_SITE        295
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:Q9I4E3"
FT   BINDING         102
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:Q9I4E3"
FT   BINDING         127
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:Q9I4E3"
FT   BINDING         172
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:Q9I4E3"
SQ   SEQUENCE   302 AA;  32960 MW;  5AE40058ACDE55E2 CRC64;
     MENTQRPSFD CEIRAKYRWF MTDSYVAAAR LGSPARRTPR TRRYAMTPPA FFAVAYAINP
     WMDVTAPVDV QVAQAQWEHL HQTYLRLGHS VDLIEPISGL PDMVYTANGG FIAHDIAVVA
     RFRFPERAGE SRAYASWMSS VGYRPVTTRH VNEGQGDLLM VGERVLAGYG FRTDQRAHAE
     IAAVLGLPVV SLELVDPRFY HLDTALAVLD DHTIAYYPPA FSTAAQEQLS ALFPDAIVVG
     SADAFVFGLN AVSDGLNVVL PVAAMGFAAQ LRAAGFEPVG VDLSELLKGG GSVKCCTLEI
     HP