DDB2_CHICK
ID DDB2_CHICK Reviewed; 507 AA.
AC Q5ZJL7;
DT 23-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT 23-NOV-2004, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=DNA damage-binding protein 2;
DE AltName: Full=Damage-specific DNA-binding protein 2;
GN Name=DDB2; ORFNames=RCJMB04_17d21;
OS Gallus gallus (Chicken).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Phasianinae; Gallus.
OX NCBI_TaxID=9031;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=CB; TISSUE=Bursa of Fabricius;
RX PubMed=15642098; DOI=10.1186/gb-2004-6-1-r6;
RA Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P.,
RA Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P.,
RA Hayashizaki Y., Buerstedde J.-M.;
RT "Full-length cDNAs from chicken bursal lymphocytes to facilitate gene
RT function analysis.";
RL Genome Biol. 6:R6.1-R6.9(2005).
CC -!- FUNCTION: Protein, which is both involved in DNA repair and protein
CC ubiquitination, as part of the UV-DDB complex and DCX (DDB1-CUL4-X-box)
CC complexes, respectively. Core component of the UV-DDB complex (UV-
CC damaged DNA-binding protein complex), a complex that recognizes UV-
CC induced DNA damage and recruit proteins of the nucleotide excision
CC repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB
CC complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-
CC 4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also
CC functions as the substrate recognition module for the DCX (DDB2-CUL4-X-
CC box) E3 ubiquitin-protein ligase complex DDB2-CUL4-ROC1 (also known as
CC CUL4-DDB-ROC1 and CUL4-DDB-RBX1). The DDB2-CUL4-ROC1 complex may
CC ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-
CC induced DNA damage. The ubiquitination of histones may facilitate their
CC removal from the nucleosome and promote subsequent DNA repair.
CC {ECO:0000250|UniProtKB:Q92466}.
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- SUBUNIT: Component of the UV-DDB complex which includes DDB1 and DDB2
CC (By similarity). Component of the DCX (DDB1-CUL4-X-box) E3 ubiquitin-
CC protein ligase complex DDB1-CUL4-ROC1 (also known as CUL4-DDB-ROC1 and
CC CUL4-DDB-RBX1), which includes CUL4A or CUL4B, DDB1, DDB2 and RBX1.
CC DDB2 may function as the substrate recognition module within this
CC complex. A large number of other DCX complexes may also exist in which
CC an alternate substrate targeting subunit replaces DDB2. These targeting
CC subunits are generally known as DCAF (DDB1- and CUL4-associated factor)
CC or CDW (CUL4-DDB1-associated WD40-repeat) proteins (By similarity).
CC {ECO:0000250|UniProtKB:Q92466}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q92466}.
CC Chromosome {ECO:0000250|UniProtKB:Q92466}. Note=Accumulates at sites of
CC DNA damage following UV irradiation. {ECO:0000250|UniProtKB:Q92466}.
CC -!- DOMAIN: The DWD box is required for interaction with DDB1.
CC {ECO:0000250|UniProtKB:Q92466}.
CC -!- DOMAIN: Interblade loops of the WD repeat region mediate most of the
CC interaction with DNA. A hairpin between blades 5 and 6 inserts into DNA
CC minor groove and mediates recognition of lesions and separation of the
CC damaged and undamaged strands (By similarity).
CC {ECO:0000250|UniProtKB:Q92466}.
CC -!- SIMILARITY: Belongs to the WD repeat DDB2/WDR76 family. {ECO:0000305}.
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DR EMBL; AJ720417; CAG32076.1; -; mRNA.
DR RefSeq; NP_001034390.1; NM_001039301.1.
DR RefSeq; XP_015142508.1; XM_015287022.1.
DR RefSeq; XP_015142509.1; XM_015287023.1.
DR RefSeq; XP_015142510.1; XM_015287024.1.
DR RefSeq; XP_015142511.1; XM_015287025.1.
DR AlphaFoldDB; Q5ZJL7; -.
DR SMR; Q5ZJL7; -.
DR STRING; 9031.ENSGALP00000013362; -.
DR PaxDb; Q5ZJL7; -.
DR Ensembl; ENSGALT00000013377; ENSGALP00000013362; ENSGALG00000008218.
DR GeneID; 423185; -.
DR KEGG; gga:423185; -.
DR CTD; 1643; -.
DR VEuPathDB; HostDB:geneid_423185; -.
DR eggNOG; KOG4328; Eukaryota.
DR GeneTree; ENSGT00510000047881; -.
DR HOGENOM; CLU_036401_0_0_1; -.
DR InParanoid; Q5ZJL7; -.
DR OMA; FIKGKGP; -.
DR OrthoDB; 559605at2759; -.
DR PhylomeDB; Q5ZJL7; -.
DR Reactome; R-GGA-5689880; Ub-specific processing proteases.
DR Reactome; R-GGA-5696394; DNA Damage Recognition in GG-NER.
DR Reactome; R-GGA-5696395; Formation of Incision Complex in GG-NER.
DR Reactome; R-GGA-5696400; Dual Incision in GG-NER.
DR Reactome; R-GGA-8951664; Neddylation.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q5ZJL7; -.
DR Proteomes; UP000000539; Chromosome 5.
DR Bgee; ENSGALG00000008218; Expressed in spermatid and 13 other tissues.
DR ExpressionAtlas; Q5ZJL7; baseline and differential.
DR GO; GO:0080008; C:Cul4-RING E3 ubiquitin ligase complex; IEA:InterPro.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0090734; C:site of DNA damage; ISS:UniProtKB.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0006281; P:DNA repair; IBA:GO_Central.
DR GO; GO:0006289; P:nucleotide-excision repair; ISS:UniProtKB.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR GO; GO:0009411; P:response to UV; IBA:GO_Central.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR033312; DDB2.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR15169; PTHR15169; 1.
DR Pfam; PF00400; WD40; 1.
DR SMART; SM00320; WD40; 4.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 2: Evidence at transcript level;
KW Chromosome; DNA damage; DNA repair; DNA-binding; Nucleus;
KW Reference proteome; Repeat; Ubl conjugation pathway; WD repeat.
FT CHAIN 1..507
FT /note="DNA damage-binding protein 2"
FT /id="PRO_0000351089"
FT REPEAT 129..164
FT /note="WD 1"
FT REPEAT 172..207
FT /note="WD 2"
FT REPEAT 223..258
FT /note="WD 3"
FT REPEAT 264..307
FT /note="WD 4"
FT REPEAT 310..350
FT /note="WD 5"
FT REPEAT 364..407
FT /note="WD 6"
FT REPEAT 417..441
FT /note="WD 7"
FT REGION 1..32
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 81..92
FT /note="Required for interaction with DDB1"
FT /evidence="ECO:0000250|UniProtKB:Q92466"
FT REGION 100..111
FT /note="Required for interaction with DDB1"
FT /evidence="ECO:0000250|UniProtKB:Q92466"
FT REGION 355..357
FT /note="Photolesion recognition"
FT /evidence="ECO:0000250|UniProtKB:Q92466"
FT REGION 459..487
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 276..294
FT /note="DWD box"
FT COMPBIAS 460..481
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 507 AA; 57312 MW; F0137FA9B0BD8EB9 CRC64;
MAPVNQPKDK KHEKAHEHRS EEAKSAGKRK LDYEGLENEP LAKKLFLRKT SKAQEKIGWN
RGGTVMRNTR ALFHQPKWQC SIVHYVYQNM LGGSIRAQLR QCLQLPFLRS LTSYRLFRTA
SPFDRRVTCL EWHPTHPSTV AVGSKGGDII LWDYEVLTKT CFIKGKGPGD SLGDIKFSPY
EAVKLYVASG DGTLSLQDLE GRAVQVISRA PDCGHENHNV CCWYCSVDVS ASCRAVVTGD
NLGNVVLLST SGEEIWKLKL HKKKVTHVEF NSRCEWLLAT ASVDQTVKIW DLRNIKDKAN
FLHVLPHDKP VNAAYFSPTD GAKLLSTDQR NEIRVYSCSD WTKPQHLIPH PHRQFQHLTP
IKATWHPRYD LIVVGRYPDP KFPGYTVNEL RTVDIFDGNT GEMVCQLYDP NASGIISLNK
FNPMGDTLAS GMGFNILIWS REEMVMKKQE HLLKAMTEQG IGSRSLSRRG GQRQANPGTS
KLKAKLLSWE VEEMGTKTKD SKSQGRK
