ID   DDBX_BOVIN              Reviewed;         323 AA.
AC   P52898; Q2KIT3;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 123.
DE   RecName: Full=Dihydrodiol dehydrogenase 3;
DE            EC=1.-.-.-;
DE   AltName: Full=Prostaglandin F synthase;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Liver;
RA   Terada T., Hirofumi N., Hideki A.;
RL   Submitted (MAR-1995) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Liver;
RA   Suzuki T., Watanabe K., Fujii Y., Chen L., Hayaishi O.;
RL   Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Testis;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (JAN-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the aldo/keto reductase family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; D49542; BAA08493.1; -; mRNA.
DR   EMBL; D88749; BAA13690.1; -; mRNA.
DR   EMBL; BC112519; AAI12520.1; -; mRNA.
DR   RefSeq; NP_851370.1; NM_181027.2.
DR   AlphaFoldDB; P52898; -.
DR   SMR; P52898; -.
DR   STRING; 9913.ENSBTAP00000014044; -.
DR   PaxDb; P52898; -.
DR   PeptideAtlas; P52898; -.
DR   PRIDE; P52898; -.
DR   Ensembl; ENSBTAT00000014044; ENSBTAP00000014044; ENSBTAG00000022564.
DR   GeneID; 282138; -.
DR   KEGG; bta:282138; -.
DR   CTD; 1109; -.
DR   VEuPathDB; HostDB:ENSBTAG00000022564; -.
DR   eggNOG; KOG1577; Eukaryota.
DR   GeneTree; ENSGT00940000153677; -.
DR   InParanoid; P52898; -.
DR   OMA; QIAMERG; -.
DR   OrthoDB; 1016440at2759; -.
DR   TreeFam; TF106492; -.
DR   Proteomes; UP000009136; Chromosome 13.
DR   Bgee; ENSBTAG00000022564; Expressed in liver and 88 other tissues.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0004032; F:alditol:NADP+ 1-oxidoreductase activity; IBA:GO_Central.
DR   GO; GO:0047023; F:androsterone dehydrogenase activity; IBA:GO_Central.
DR   GO; GO:0032052; F:bile acid binding; IBA:GO_Central.
DR   GO; GO:0047086; F:ketosteroid monooxygenase activity; IBA:GO_Central.
DR   GO; GO:0016229; F:steroid dehydrogenase activity; IBA:GO_Central.
DR   GO; GO:0044597; P:daunorubicin metabolic process; IBA:GO_Central.
DR   GO; GO:0044598; P:doxorubicin metabolic process; IBA:GO_Central.
DR   GO; GO:0042448; P:progesterone metabolic process; IBA:GO_Central.
DR   GO; GO:0006693; P:prostaglandin metabolic process; IBA:GO_Central.
DR   GO; GO:0008202; P:steroid metabolic process; IBA:GO_Central.
DR   CDD; cd19108; AKR_AKR1C1-35; 1.
DR   Gene3D; 3.20.20.100; -; 1.
DR   InterPro; IPR020471; AKR.
DR   InterPro; IPR044482; AKR1C.
DR   InterPro; IPR018170; Aldo/ket_reductase_CS.
DR   InterPro; IPR023210; NADP_OxRdtase_dom.
DR   InterPro; IPR036812; NADP_OxRdtase_dom_sf.
DR   Pfam; PF00248; Aldo_ket_red; 1.
DR   PIRSF; PIRSF000097; AKR; 1.
DR   PRINTS; PR00069; ALDKETRDTASE.
DR   SUPFAM; SSF51430; SSF51430; 1.
DR   PROSITE; PS00798; ALDOKETO_REDUCTASE_1; 1.
DR   PROSITE; PS00062; ALDOKETO_REDUCTASE_2; 1.
DR   PROSITE; PS00063; ALDOKETO_REDUCTASE_3; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; NADP; Oxidoreductase; Reference proteome.
FT   CHAIN           1..323
FT                   /note="Dihydrodiol dehydrogenase 3"
FT                   /id="PRO_0000124648"
FT   ACT_SITE        55
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   BINDING         20..24
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         50
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         117
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         166..167
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         190
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         216..221
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   BINDING         270..280
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   SITE            84
FT                   /note="Lowers pKa of active site Tyr"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   323 AA;  36784 MW;  B7FCC6F8EE09A010 CRC64;
     MDPKGQRVKL NDGHFIPVLG FGTFAPREVP KSEALEVTKF AIEAGFRHID SAHLYQNEEQ
     VGQAIRSKIA DGTVKREDIF YTSKLWSTSL RPELVRPALE KSLNNLQLDY VDLYIIHFPV
     ALKPGETLFP TDENGKPIFD SVDLCRTWEA LEKCKDAGLT KSIGVSNFNH KQLEKILNKP
     GLKYKPVCNQ VECHPYFNQS KLLDFCKSHD IVLVAYGALG SQRLKEWVNP NLPFLLEDPV
     LSAIAKKHRQ TPALVALRYQ IQRGVVVLAK SYNKKRIKEN IQVFDFELTP EDMKAIDGLN
     SNMRYNELLL GVGHPEYPFV EEY