ID   DDGLD_PSEA6             Reviewed;         254 AA.
AC   Q15SS0;
DT   17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT   25-JUL-2006, sequence version 1.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase {ECO:0000305};
DE            EC=1.1.1.127 {ECO:0000269|Ref.2};
DE   AltName: Full=2,5-diketo-3-deoxy-L-galactonate 5-reductase {ECO:0000303|Ref.2};
GN   OrderedLocusNames=Patl_2552 {ECO:0000312|EMBL:ABG41068.1};
OS   Pseudoalteromonas atlantica (strain T6c / ATCC BAA-1087).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Pseudoalteromonadaceae; Pseudoalteromonas.
OX   NCBI_TaxID=342610;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=T6c / ATCC BAA-1087;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Saunders E., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Karls A.C.,
RA   Bartlett D., Higgins B.P., Richardson P.;
RT   "Complete sequence of Pseudoalteromonas atlantica T6c.";
RL   Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, AND CATALYTIC ACTIVITY.
RC   STRAIN=T6c / ATCC BAA-1087;
RX   DOI=10.1007/s12257-014-0622-3;
RA   Lee S.B., Cho S.J., Kim J.A., Lee S.Y., Kim S.M., Lim H.S.;
RT   "Metabolic pathway of 3,6-anhydro-L-galactose in agar-degrading
RT   microorganisms.";
RL   Biotechnol. Bioprocess Eng. 19:866-878(2014).
CC   -!- FUNCTION: Involved in the degradation of 3,6-anhydro-L-galactose, which
CC       is the major monomeric sugar of red macroalgae. Catalyzes the fourth
CC       step of the pathway, the reduction of 3-deoxy-D-glycero-2,5-
CC       hexodiulosonate (L-DDGal) to 2-dehydro-3-deoxy-D-gluconate (KDG).
CC       {ECO:0000269|Ref.2}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2-dehydro-3-deoxy-D-gluconate + NAD(+) = 3-deoxy-D-glycero-
CC         2,5-hexodiulosonate + H(+) + NADH; Xref=Rhea:RHEA:24232,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:29071, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945, ChEBI:CHEBI:57990; EC=1.1.1.127;
CC         Evidence={ECO:0000269|Ref.2};
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
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DR   EMBL; CP000388; ABG41068.1; -; Genomic_DNA.
DR   RefSeq; WP_011575334.1; NC_008228.1.
DR   AlphaFoldDB; Q15SS0; -.
DR   SMR; Q15SS0; -.
DR   STRING; 342610.Patl_2552; -.
DR   EnsemblBacteria; ABG41068; ABG41068; Patl_2552.
DR   KEGG; pat:Patl_2552; -.
DR   eggNOG; COG1028; Bacteria.
DR   HOGENOM; CLU_010194_1_1_6; -.
DR   OMA; RIWDSKV; -.
DR   OrthoDB; 1294334at2; -.
DR   BioCyc; MetaCyc:MON-19467; -.
DR   Proteomes; UP000001981; Chromosome.
DR   GO; GO:0047001; F:2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR020904; Sc_DH/Rdtase_CS.
DR   InterPro; IPR002347; SDR_fam.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   PROSITE; PS00061; ADH_SHORT; 1.
PE   1: Evidence at protein level;
KW   Carbohydrate metabolism; NAD; Oxidoreductase; Reference proteome.
FT   CHAIN           1..254
FT                   /note="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase"
FT                   /id="PRO_0000449955"
FT   ACT_SITE        159
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10001"
SQ   SEQUENCE   254 AA;  26864 MW;  F386C0EF0DBCB0BA CRC64;
     MLEKFSLEGK VALVTGCKRG IGKGIALGLA EAGADIIGVS ASLALEGSDV ENEVKALGRN
     FKGYQCDFSD RDALYAFIKE VKADFPKIDI LVNNAGTILR APAAEHGDDL WDKVIDVNLN
     SQFILSREIG KEMVARQSGK IIFTASLLTF QGGITVPGYA ASKGAIGQLV MALSNEWAGK
     GVNVNAIAPG YIDTDNTQAL REDSERSAAI LGRIPQGRWG NPDDFKGPAV FLASDAASYV
     NGAILLVDGG WMGR