ID   DDH2_SCHPO              Reviewed;         332 AA.
AC   Q9P7Q1;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=2-hydroxyacid dehydrogenase homolog 2;
DE            EC=1.1.1.-;
GN   ORFNames=SPAC186.02c;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
CC   -!- SIMILARITY: Belongs to the D-isomer specific 2-hydroxyacid
CC       dehydrogenase family. {ECO:0000305}.
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DR   EMBL; CU329670; CAB75866.1; -; Genomic_DNA.
DR   PIR; T50129; T50129.
DR   RefSeq; NP_595020.1; NM_001020451.2.
DR   AlphaFoldDB; Q9P7Q1; -.
DR   SMR; Q9P7Q1; -.
DR   STRING; 4896.SPAC186.02c.1; -.
DR   PaxDb; Q9P7Q1; -.
DR   PRIDE; Q9P7Q1; -.
DR   EnsemblFungi; SPAC186.02c.1; SPAC186.02c.1:pep; SPAC186.02c.
DR   GeneID; 2542495; -.
DR   KEGG; spo:SPAC186.02c; -.
DR   PomBase; SPAC186.02c; -.
DR   VEuPathDB; FungiDB:SPAC186.02c; -.
DR   eggNOG; KOG0068; Eukaryota.
DR   HOGENOM; CLU_019796_1_1_1; -.
DR   InParanoid; Q9P7Q1; -.
DR   OMA; VIVTAHQ; -.
DR   PhylomeDB; Q9P7Q1; -.
DR   PRO; PR:Q9P7Q1; -.
DR   Proteomes; UP000002485; Chromosome I.
DR   GO; GO:0005829; C:cytosol; HDA:PomBase.
DR   GO; GO:0005634; C:nucleus; HDA:PomBase.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IEA:InterPro.
DR   InterPro; IPR006139; D-isomer_2_OHA_DH_cat_dom.
DR   InterPro; IPR029753; D-isomer_DH_CS.
DR   InterPro; IPR029752; D-isomer_DH_CS1.
DR   InterPro; IPR006140; D-isomer_DH_NAD-bd.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   Pfam; PF00389; 2-Hacid_dh; 1.
DR   Pfam; PF02826; 2-Hacid_dh_C; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
DR   PROSITE; PS00065; D_2_HYDROXYACID_DH_1; 1.
DR   PROSITE; PS00670; D_2_HYDROXYACID_DH_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; NAD; Nucleus; Oxidoreductase; Reference proteome.
FT   CHAIN           1..332
FT                   /note="2-hydroxyacid dehydrogenase homolog 2"
FT                   /id="PRO_0000316031"
FT   ACT_SITE        235
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        264
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        296
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   BINDING         154..155
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         233..235
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         259
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         296..299
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   332 AA;  36369 MW;  F886902386782C40 CRC64;
     MRVVLFSSQS YDRGPFEEAN KTFNHEIIYH NFSLNKDTVS LAGKAQVVCV FVNDQVDADT
     LKALAENGVK LVALRCGGYN NVNLKAASEY KITVVHVPSY SPFAVSEFTV GLLLSLNRKI
     HRAYVRVRED DFNIVGLLGC DIHGKTVGVI GTGKIGSNVA KCFKMGFGCD VLAYDINPDK
     KLENYGVQFV EQNEVLKKAD FLCLHCPLTP STTHIVNSDS LALMKKGVTI VNTSRGGLID
     TKALVDAIDS GQVGGCAIDV YEGERNLFYK DLSNEVIKDS TFQRLVNFPN VLVTSHQAFF
     TTEALCSIAH TTLKSASDFY TNSLDESVIA NK