ACUM_ASPFU
ID ACUM_ASPFU Reviewed; 627 AA.
AC E5DG73; Q4X0T8;
DT 20-JUN-2018, integrated into UniProtKB/Swiss-Prot.
DT 20-JUN-2018, sequence version 2.
DT 03-AUG-2022, entry version 38.
DE RecName: Full=Zinc cluster transcription factor acuM {ECO:0000303|PubMed:21062375};
DE AltName: Full=Iron acquisition regulator acuM {ECO:0000305};
GN Name=AcuM {ECO:0000303|PubMed:21062375}; ORFNames=AFUA_2g12330;
OS Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC
OS A1100) (Aspergillus fumigatus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Fumigati.
OX NCBI_TaxID=330879;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100;
RX PubMed=16372009; DOI=10.1038/nature04332;
RA Nierman W.C., Pain A., Anderson M.J., Wortman J.R., Kim H.S., Arroyo J.,
RA Berriman M., Abe K., Archer D.B., Bermejo C., Bennett J.W., Bowyer P.,
RA Chen D., Collins M., Coulsen R., Davies R., Dyer P.S., Farman M.L.,
RA Fedorova N., Fedorova N.D., Feldblyum T.V., Fischer R., Fosker N.,
RA Fraser A., Garcia J.L., Garcia M.J., Goble A., Goldman G.H., Gomi K.,
RA Griffith-Jones S., Gwilliam R., Haas B.J., Haas H., Harris D.E.,
RA Horiuchi H., Huang J., Humphray S., Jimenez J., Keller N., Khouri H.,
RA Kitamoto K., Kobayashi T., Konzack S., Kulkarni R., Kumagai T., Lafton A.,
RA Latge J.-P., Li W., Lord A., Lu C., Majoros W.H., May G.S., Miller B.L.,
RA Mohamoud Y., Molina M., Monod M., Mouyna I., Mulligan S., Murphy L.D.,
RA O'Neil S., Paulsen I., Penalva M.A., Pertea M., Price C., Pritchard B.L.,
RA Quail M.A., Rabbinowitsch E., Rawlins N., Rajandream M.A., Reichard U.,
RA Renauld H., Robson G.D., Rodriguez de Cordoba S., Rodriguez-Pena J.M.,
RA Ronning C.M., Rutter S., Salzberg S.L., Sanchez M., Sanchez-Ferrero J.C.,
RA Saunders D., Seeger K., Squares R., Squares S., Takeuchi M., Tekaia F.,
RA Turner G., Vazquez de Aldana C.R., Weidman J., White O., Woodward J.R.,
RA Yu J.-H., Fraser C.M., Galagan J.E., Asai K., Machida M., Hall N.,
RA Barrell B.G., Denning D.W.;
RT "Genomic sequence of the pathogenic and allergenic filamentous fungus
RT Aspergillus fumigatus.";
RL Nature 438:1151-1156(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 175-627, FUNCTION, DISRUPTION PHENOTYPE, AND
RP INDUCTION.
RC STRAIN=ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100;
RX PubMed=21062375; DOI=10.1111/j.1365-2958.2010.07389.x;
RA Liu H., Gravelat F.N., Chiang L.Y., Chen D., Vanier G., Ejzykowicz D.E.,
RA Ibrahim A.S., Nierman W.C., Sheppard D.C., Filler S.G.;
RT "Aspergillus fumigatus AcuM regulates both iron acquisition and
RT gluconeogenesis.";
RL Mol. Microbiol. 78:1038-1054(2010).
CC -!- FUNCTION: Transcription factor that governs genes involved in reductive
CC and siderophore-mediated iron acquisition, and carbon metabolism
CC (PubMed:21062375). Suppresses the expression of sreA and induces hapX
CC to stimulate expression of genes involved in both reductive iron
CC assimilation and siderophore-mediated iron uptake which is essential
CC for the maximal virulence (PubMed:21062375). Regulates also genes
CC involved in gluconeogenesis (PubMed:21062375).
CC {ECO:0000269|PubMed:21062375}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- INDUCTION: Expressed constitutively with similar lebels in swollen
CC conidia, germlings and hyphae (PubMed:21062375).
CC {ECO:0000269|PubMed:21062375}.
CC -!- DISRUPTION PHENOTYPE: Leads to attenuated virulence in mouse models of
CC haematogenously disseminated and invasive pulmonary aspergillosis
CC (PubMed:21062375). Results in increased expression of sreA and reduced
CC expression of hapX iron acquisition transcription factors
CC (PubMed:21062375). Impairs also gluconeogenesis (PubMed:21062375).
CC {ECO:0000269|PubMed:21062375}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EAL93527.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AAHF01000001; EAL93527.1; ALT_SEQ; Genomic_DNA.
DR EMBL; GU290314; ADK36628.1; -; mRNA.
DR RefSeq; XP_755565.1; XM_750472.1.
DR AlphaFoldDB; E5DG73; -.
DR STRING; 746128.CADAFUBP00002738; -.
DR EnsemblFungi; EAL93527; EAL93527; AFUA_2G12330.
DR GeneID; 3513314; -.
DR KEGG; afm:AFUA_2G12330; -.
DR eggNOG; ENOG502QQGC; Eukaryota.
DR HOGENOM; CLU_010748_0_0_1; -.
DR OrthoDB; 681770at2759; -.
DR Proteomes; UP000002530; Chromosome 2.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0008152; P:metabolic process; IEA:UniProt.
DR CDD; cd00067; GAL4; 1.
DR Gene3D; 4.10.240.10; -; 1.
DR InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR Pfam; PF00172; Zn_clus; 1.
DR SMART; SM00066; GAL4; 1.
DR SUPFAM; SSF57701; SSF57701; 1.
DR PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Metal-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation; Zinc.
FT CHAIN 1..627
FT /note="Zinc cluster transcription factor acuM"
FT /id="PRO_0000444417"
FT DNA_BIND 197..225
FT /note="Zn(2)-C6 fungal-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT REGION 1..40
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 129..148
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 155..193
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 225..258
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 277..305
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 394..416
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 159..183
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 225..249
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 285..305
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 627 AA; 69854 MW; 80A884AE96FAB3AE CRC64;
MGCRKSSCPR HHTPKAGTFP PRWFPSAEEP GKKGGQPARP TVVISAGTSE HETITIARRR
RTPQLKARHL LSAPANASDL SSSKLIFDVC RSPSDLLLRV PTTNSEHQQA ELCATALRPT
RSKRLMTENG TAQTGTVPVE QPRNGTMENA KLNMAEGDSS RMESGSKNTA SPPVKADNNA
AGTHSSPKKR RKVNHACVYC RRSHMTCDSE RPCTRCIKRN IGHLCHDEPR EPSKRARSEH
EHSTAEEDGH SNNEFSNAQS MPRNVDVQDA AGQQILPDGT VALPPSSVSA VQHNTIPSSS
AQNSLGHNSQ QLLGYNEWLG GQSQFQDMHT FHPSYMFNAP EVTNEYNLLG DFLSSSLLDD
GGMFSNDNLQ GIYSDPTLIN SMANLDNTAL LQQAQPSQPT QSQPHQNDSV QGPSSTVVND
KARETYYMTA ADPSGSDPPE ERMNKLLKAK YDAGLLKPFN YVKGYARLNQ YMEKNMKQSS
RQKILRQLDK FRPKFRERMQ SLTDIELILV EMWFERSLME YDRVFASMAI PACCWRRTGE
IFRGNKEMAE LIGVPIESLR DGKLAIHEII VEDQLVSYWE KFGAIAFDNT QKAMLTSCTL
KNPNSSNPGN GIPCCFSFTI RRDNHNM
