ID   DDPS2_ARATH             Reviewed;         310 AA.
AC   Q56Y11; Q9LUY4;
DT   13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 2.
DT   25-MAY-2022, entry version 110.
DE   RecName: Full=Dehydrodolichyl diphosphate synthase 2;
DE            Short=Dedol-PP synthase 2;
DE            EC=2.5.1.-;
GN   OrderedLocusNames=At5g58770; ORFNames=MZN1.21;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA   Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT   features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT   clones.";
RL   DNA Res. 7:31-63(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA   Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes cis-prenyl chain elongation to produce the
CC       polyprenyl backbone of dolichol, a glycosyl carrier-lipid required for
CC       the biosynthesis of several classes of glycoprotein. {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC   -!- PATHWAY: Protein modification; protein glycosylation.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=Q56Y11-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=Q56Y11-2; Sequence=VSP_015652, VSP_015653;
CC   -!- MISCELLANEOUS: [Isoform 2]: May be due to an intron retention.
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the UPP synthase family. {ECO:0000305}.
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DR   EMBL; AB020755; BAA97345.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED97096.1; -; Genomic_DNA.
DR   EMBL; AK221512; BAD94770.1; -; mRNA.
DR   RefSeq; NP_200685.1; NM_125264.3. [Q56Y11-1]
DR   AlphaFoldDB; Q56Y11; -.
DR   SMR; Q56Y11; -.
DR   BioGRID; 21235; 1.
DR   STRING; 3702.AT5G58770.1; -.
DR   PaxDb; Q56Y11; -.
DR   PRIDE; Q56Y11; -.
DR   ProteomicsDB; 224663; -. [Q56Y11-1]
DR   EnsemblPlants; AT5G58770.1; AT5G58770.1; AT5G58770. [Q56Y11-1]
DR   GeneID; 835991; -.
DR   Gramene; AT5G58770.1; AT5G58770.1; AT5G58770. [Q56Y11-1]
DR   KEGG; ath:AT5G58770; -.
DR   Araport; AT5G58770; -.
DR   TAIR; locus:2178823; AT5G58770.
DR   eggNOG; KOG1602; Eukaryota.
DR   HOGENOM; CLU_038505_1_0_1; -.
DR   InParanoid; Q56Y11; -.
DR   OMA; PRTEGHK; -.
DR   PhylomeDB; Q56Y11; -.
DR   UniPathway; UPA00378; -.
DR   PRO; PR:Q56Y11; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q56Y11; baseline and differential.
DR   Genevisible; Q56Y11; AT.
DR   GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR   GO; GO:0009570; C:chloroplast stroma; IDA:TAIR.
DR   GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR   GO; GO:0045547; F:dehydrodolichyl diphosphate synthase activity; IBA:GO_Central.
DR   GO; GO:0002094; F:polyprenyltransferase activity; IDA:TAIR.
DR   GO; GO:0009668; P:plastid membrane organization; IMP:TAIR.
DR   GO; GO:0016094; P:polyprenol biosynthetic process; IBA:GO_Central.
DR   GO; GO:0006486; P:protein glycosylation; IEA:UniProtKB-UniPathway.
DR   GO; GO:0009409; P:response to cold; IBA:GO_Central.
DR   CDD; cd00475; Cis_IPPS; 1.
DR   Gene3D; 3.40.1180.10; -; 1.
DR   HAMAP; MF_01139; ISPT; 1.
DR   InterPro; IPR001441; UPP_synth-like.
DR   InterPro; IPR018520; UPP_synth-like_CS.
DR   InterPro; IPR036424; UPP_synth-like_sf.
DR   PANTHER; PTHR10291; PTHR10291; 1.
DR   Pfam; PF01255; Prenyltransf; 1.
DR   SUPFAM; SSF64005; SSF64005; 1.
DR   TIGRFAMs; TIGR00055; uppS; 1.
DR   PROSITE; PS01066; UPP_SYNTHASE; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Reference proteome; Transferase.
FT   CHAIN           1..310
FT                   /note="Dehydrodolichyl diphosphate synthase 2"
FT                   /id="PRO_0000123752"
FT   VAR_SEQ         140..145
FT                   /note="IEIDFL -> VSDKPN (in isoform 2)"
FT                   /evidence="ECO:0000303|Ref.3"
FT                   /id="VSP_015652"
FT   VAR_SEQ         146..310
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|Ref.3"
FT                   /id="VSP_015653"
SQ   SEQUENCE   310 AA;  35943 MW;  B73A3A15C59A71E0 CRC64;
     MLSLRVPTPT SFDFRRYQAG DLERRWRLSR DSFLSFSPKF EENRGFRFGV KSSKSDVSFT
     AAEEEETLPE ELHAELMPKH VAIIMDGNGR WAKNRGLQPW DGHRAGVEAL KEIVELCGKW
     GIQVLTVFAF STDNWIRPRI EIDFLFSLFE RSLKTEFQNL AKNNVRISII GDSSKLPKSL
     LRVINEVEEV TKNNTRLQLI VAVGYSGKYD VLQACRGIAR RVKDGEIEVE EIDERLIEEE
     LETNCTEFPY PDLLIRTSGE LRVSNFLLWQ LAYTELFFAQ ELWPDFGRSG FIEALMSFQQ
     RQRRFGGRKS