3BHS_CANLF
ID 3BHS_CANLF Reviewed; 373 AA.
AC Q5IFP1;
DT 22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase;
DE Short=3-beta-HSD;
DE Includes:
DE RecName: Full=3-beta-hydroxy-Delta(5)-steroid dehydrogenase;
DE EC=1.1.1.145;
DE AltName: Full=3-beta-hydroxy-5-ene steroid dehydrogenase;
DE AltName: Full=Progesterone reductase;
DE Includes:
DE RecName: Full=Steroid Delta-isomerase;
DE EC=5.3.3.1;
DE AltName: Full=Delta-5-3-ketosteroid isomerase;
GN Name=HSD3B;
OS Canis lupus familiaris (Dog) (Canis familiaris).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Carnivora; Caniformia; Canidae; Canis.
OX NCBI_TaxID=9615;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Corpus luteum;
RA Kowalewski M.P., Taubert A., Hoffmann B.;
RT "Expression of 3-beta-hydroxysteroid dehydrogenase in canine corpus luteum
RT during dioestrus.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: 3-beta-HSD is a bifunctional enzyme, that catalyzes the
CC oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the
CC oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system
CC plays a crucial role in the biosynthesis of all classes of hormonal
CC steroids (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 3beta-hydroxy-Delta(5)-steroid + NAD(+) = a 3-oxo-Delta(5)-
CC steroid + H(+) + NADH; Xref=Rhea:RHEA:24076, ChEBI:CHEBI:1722,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:47907, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945; EC=1.1.1.145;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid;
CC Xref=Rhea:RHEA:14709, ChEBI:CHEBI:47907, ChEBI:CHEBI:47909;
CC EC=5.3.3.1;
CC -!- PATHWAY: Lipid metabolism; steroid biosynthesis.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC Single-pass membrane protein {ECO:0000250}. Mitochondrion membrane
CC {ECO:0000250}; Single-pass membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the 3-beta-HSD family. {ECO:0000305}.
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DR EMBL; AY739720; AAW31741.1; -; mRNA.
DR RefSeq; NP_001010954.1; NM_001010954.2.
DR AlphaFoldDB; Q5IFP1; -.
DR SMR; Q5IFP1; -.
DR STRING; 9615.ENSCAFP00000014770; -.
DR PaxDb; Q5IFP1; -.
DR Ensembl; ENSCAFT00030012465; ENSCAFP00030010903; ENSCAFG00030006775.
DR Ensembl; ENSCAFT00040026459; ENSCAFP00040022992; ENSCAFG00040014327.
DR Ensembl; ENSCAFT00845011264; ENSCAFP00845008786; ENSCAFG00845006351.
DR GeneID; 483146; -.
DR KEGG; cfa:483146; -.
DR CTD; 3284; -.
DR VEuPathDB; HostDB:ENSCAFG00845006351; -.
DR eggNOG; KOG1430; Eukaryota.
DR GeneTree; ENSGT00940000155444; -.
DR InParanoid; Q5IFP1; -.
DR OrthoDB; 930591at2759; -.
DR Reactome; R-CFA-193048; Androgen biosynthesis.
DR Reactome; R-CFA-193993; Mineralocorticoid biosynthesis.
DR Reactome; R-CFA-194002; Glucocorticoid biosynthesis.
DR UniPathway; UPA00062; -.
DR Proteomes; UP000002254; Chromosome 17.
DR Bgee; ENSCAFG00000010039; Expressed in adrenal cortex and 25 other tissues.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IBA:GO_Central.
DR GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0003854; F:3-beta-hydroxy-delta5-steroid dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0102294; F:cholesterol dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IBA:GO_Central.
DR GO; GO:0004769; F:steroid delta-isomerase activity; IEA:UniProtKB-EC.
DR GO; GO:0008207; P:C21-steroid hormone metabolic process; IBA:GO_Central.
DR GO; GO:0021766; P:hippocampus development; IBA:GO_Central.
DR GO; GO:0051412; P:response to corticosterone; IBA:GO_Central.
DR GO; GO:0006694; P:steroid biosynthetic process; IBA:GO_Central.
DR InterPro; IPR002225; 3Beta_OHSteriod_DH/Estase.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR Pfam; PF01073; 3Beta_HSD; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Isomerase; Membrane; Mitochondrion;
KW Multifunctional enzyme; NAD; Oxidoreductase; Reference proteome;
KW Steroidogenesis; Transmembrane; Transmembrane helix.
FT CHAIN 1..373
FT /note="3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-
FT isomerase"
FT /id="PRO_0000087772"
FT TRANSMEM 288..308
FT /note="Helical"
FT /evidence="ECO:0000255"
FT ACT_SITE 155
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT BINDING 159
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
SQ SEQUENCE 373 AA; 42378 MW; EB12CB016D650A5B CRC64;
MAGWSCLVTG AGGFLGQRIV HLLAEEKELQ EIRALDKAFR PELLEEFSKL QSKTKLTMVE
GDILDEQCLK RACQGTSVVI HTASVIDVMN VIHRETIMNV NLKGTQLLLE ACAQASVPIF
IYTSTIEVAG PNSYRDIIQN AHEEEHLEST WSAPYPYSKK LAEKAVLAAN GWALKNGGTL
HTCALRPMYI YGEGSIFLYN YIYKALRNNG ILTHHSKFSI VNPVYVGNVA WAHILALRAL
QDPKKAPSVQ GQFYYISDDT PHQSYDDLNY NLSKEWGFSL DSRMSLPISL EYWLAFLLEI
VSFLLSPIYK YQPPFNRHMV TLSNSIFTFS YKKAQRDLGY KPLFSWEEAK QKTTEWIGSL
VKQHKETLKT KTH
