ID   DDRB_DEIGD              Reviewed;         178 AA.
AC   Q1J1N6;
DT   15-JUN-2010, integrated into UniProtKB/Swiss-Prot.
DT   13-JUN-2006, sequence version 1.
DT   25-MAY-2022, entry version 61.
DE   RecName: Full=Single-stranded DNA-binding protein DdrB;
DE   AltName: Full=DNA damage response protein B;
GN   Name=ddrB; OrderedLocusNames=Dgeo_0295;
OS   Deinococcus geothermalis (strain DSM 11300 / AG-3a).
OC   Bacteria; Deinococcus-Thermus; Deinococci; Deinococcales; Deinococcaceae;
OC   Deinococcus.
OX   NCBI_TaxID=319795;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 11300 / AG-3a;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Brettin T., Bruce D., Han C., Tapia R., Saunders E., Gilna P., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Daly M.J.,
RA   Fredrickson J.K., Makarova K.S., Gaidamakova E.K., Zhai M., Richardson P.;
RT   "Complete sequence of chromosome 1 of Deinococcus geothermalis DSM 11300.";
RL   Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS), FUNCTION AS A SSB PROTEIN, SUBUNIT,
RP   AND DOMAIN.
RX   PubMed=20129942; DOI=10.1093/nar/gkq036;
RA   Sugiman-Marangos S., Junop M.S.;
RT   "The structure of DdrB from Deinococcus: a new fold for single-stranded DNA
RT   binding proteins.";
RL   Nucleic Acids Res. 38:3432-3440(2010).
CC   -!- FUNCTION: ssDNA-binding protein that probably contributes to the
CC       ionizing radiation resistance of D.geothermalis. Plays a role in DNA
CC       repair and genome reconstitution, in a RecA-independent process, and is
CC       necessary for recovery from severe genomic fragmentation as a result of
CC       exposure to severe levels of ionizing radiation (Probable). Binds
CC       single-stranded DNA but not duplex DNA. {ECO:0000269|PubMed:20129942,
CC       ECO:0000305}.
CC   -!- SUBUNIT: Homopentamer; arranged in a ring-structure.
CC       {ECO:0000269|PubMed:20129942}.
CC   -!- INDUCTION: Induced to high levels following extreme ionizing radiation
CC       exposure. Also highly induced in response to desiccation stress (By
CC       similarity). {ECO:0000250}.
CC   -!- DOMAIN: Contains a novel ssDNA-binding fold, which is structurally and
CC       topologically distinct from the OB-fold universally found in standard
CC       SSB proteins. The disordered C-terminus of DdrB may mediate
CC       interactions with other proteins important for DNA damage recovery.
CC       {ECO:0000269|PubMed:20129942}.
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DR   EMBL; CP000359; ABF44598.1; -; Genomic_DNA.
DR   RefSeq; WP_011529443.1; NC_008025.1.
DR   PDB; 4EXW; X-ray; 2.80 A; A/B/C/D/E=1-178.
DR   PDBsum; 4EXW; -.
DR   AlphaFoldDB; Q1J1N6; -.
DR   SMR; Q1J1N6; -.
DR   EnsemblBacteria; ABF44598; ABF44598; Dgeo_0295.
DR   KEGG; dge:Dgeo_0295; -.
DR   eggNOG; ENOG50337A5; Bacteria.
DR   HOGENOM; CLU_129192_0_0_0; -.
DR   OMA; LIGARKW; -.
DR   OrthoDB; 1680534at2; -.
DR   EvolutionaryTrace; Q1J1N6; -.
DR   Proteomes; UP000002431; Chromosome.
DR   GO; GO:0003697; F:single-stranded DNA binding; IDA:UniProtKB.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   InterPro; IPR024305; ssDNA-bd_DdrB-like.
DR   Pfam; PF12747; DdrB; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA damage; DNA repair; DNA-binding; Reference proteome;
KW   Stress response.
FT   CHAIN           1..178
FT                   /note="Single-stranded DNA-binding protein DdrB"
FT                   /id="PRO_0000394492"
FT   REGION          139..178
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   STRAND          2..7
FT                   /evidence="ECO:0007829|PDB:4EXW"
FT   STRAND          13..20
FT                   /evidence="ECO:0007829|PDB:4EXW"
FT   HELIX           21..23
FT                   /evidence="ECO:0007829|PDB:4EXW"
FT   HELIX           24..32
FT                   /evidence="ECO:0007829|PDB:4EXW"
FT   TURN            33..35
FT                   /evidence="ECO:0007829|PDB:4EXW"
FT   STRAND          46..48
FT                   /evidence="ECO:0007829|PDB:4EXW"
FT   HELIX           50..52
FT                   /evidence="ECO:0007829|PDB:4EXW"
FT   HELIX           58..61
FT                   /evidence="ECO:0007829|PDB:4EXW"
FT   STRAND          64..67
FT                   /evidence="ECO:0007829|PDB:4EXW"
FT   STRAND          73..79
FT                   /evidence="ECO:0007829|PDB:4EXW"
FT   STRAND          81..87
FT                   /evidence="ECO:0007829|PDB:4EXW"
FT   STRAND          100..106
FT                   /evidence="ECO:0007829|PDB:4EXW"
FT   STRAND          125..134
FT                   /evidence="ECO:0007829|PDB:4EXW"
FT   HELIX           138..140
FT                   /evidence="ECO:0007829|PDB:4EXW"
SQ   SEQUENCE   178 AA;  19866 MW;  3FB62904DCD4B736 CRC64;
     MLHIEFITDL GAKVTVDVES ADKLLDVQRQ YGRLGWTSGE VPVGGYQFPL ENEPDFDWSL
     IGARKWTNPE GEEMILHRGH AYRRRELEAV DSRKMKLPAA VKYSRGAKNT DPEHVREKAD
     GEFEYVTLAI FRGGKRQERY AVPGSNRPQA GAPARSAATR AQGARPGAVA VQDEETPF