ID   DDRGK_DANRE             Reviewed;         300 AA.
AC   Q6P0E5;
DT   02-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=DDRGK domain-containing protein 1 {ECO:0000305};
DE   Flags: Precursor;
GN   Name=ddrgk1 {ECO:0000303|PubMed:28263186};
GN   ORFNames=zgc:56488 {ECO:0000303|Ref.1};
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=28263186; DOI=10.1172/jci90193;
RA   Egunsola A.T., Bae Y., Jiang M.M., Liu D.S., Chen-Evenson Y., Bertin T.,
RA   Chen S., Lu J.T., Nevarez L., Magal N., Raas-Rothschild A., Swindell E.C.,
RA   Cohn D.H., Gibbs R.A., Campeau P.M., Shohat M., Lee B.H.;
RT   "Loss of DDRGK1 modulates SOX9 ubiquitination in spondyloepimetaphyseal
RT   dysplasia.";
RL   J. Clin. Invest. 127:1475-1484(2017).
CC   -!- FUNCTION: Substrate adapter for ufmylation, the covalent attachment of
CC       the ubiquitin-like modifier UFM1 to substrate proteins, which plays a
CC       key role in reticulophagy (also called ER-phagy) (By similarity). In
CC       response to endoplasmic reticulum stress, promotes recruitment of the
CC       E3 ufm1-protein ligase ufl1 to the endoplasmic reticulum membrane,
CC       leading to ufmylation of target proteins and subsequent reticulophagy
CC       of endoplasmic reticulum sheets (By similarity). Plays a role in
CC       cartilage development through sox9, inhibiting the ubiquitin-mediated
CC       proteasomal degradation of this transcriptional regulator
CC       (PubMed:28263186). {ECO:0000250|UniProtKB:Q96HY6,
CC       ECO:0000269|PubMed:28263186}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum
CC       {ECO:0000250|UniProtKB:Q96HY6}. Endoplasmic reticulum membrane
CC       {ECO:0000250|UniProtKB:Q96HY6}. Note=Localizes to the endoplasmic
CC       reticulum membrane in response to endoplasmic reticulum stress.
CC       {ECO:0000250|UniProtKB:Q96HY6}.
CC   -!- DISRUPTION PHENOTYPE: Morpholino knockdown alters craniofacial
CC       cartilage development. {ECO:0000269|PubMed:28263186}.
CC   -!- SIMILARITY: Belongs to the DDRGK1 family. {ECO:0000305}.
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DR   EMBL; BC065652; AAH65652.1; -; mRNA.
DR   RefSeq; NP_956587.2; NM_200293.2.
DR   AlphaFoldDB; Q6P0E5; -.
DR   SMR; Q6P0E5; -.
DR   STRING; 7955.ENSDARP00000088891; -.
DR   PaxDb; Q6P0E5; -.
DR   PRIDE; Q6P0E5; -.
DR   GeneID; 393263; -.
DR   KEGG; dre:393263; -.
DR   CTD; 65992; -.
DR   ZFIN; ZDB-GENE-040426-1050; ddrgk1.
DR   eggNOG; KOG3054; Eukaryota.
DR   InParanoid; Q6P0E5; -.
DR   OrthoDB; 1553559at2759; -.
DR   PhylomeDB; Q6P0E5; -.
DR   Reactome; R-DRE-8980692; RHOA GTPase cycle.
DR   PRO; PR:Q6P0E5; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0005783; C:endoplasmic reticulum; ISS:UniProtKB.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; ISS:UniProtKB.
DR   GO; GO:0051216; P:cartilage development; IMP:ZFIN.
DR   GO; GO:1903895; P:negative regulation of IRE1-mediated unfolded protein response; ISS:UniProtKB.
DR   GO; GO:1990592; P:protein K69-linked ufmylation; ISS:UniProtKB.
DR   GO; GO:0070972; P:protein localization to endoplasmic reticulum; ISS:UniProtKB.
DR   GO; GO:0071569; P:protein ufmylation; ISS:UniProtKB.
DR   GO; GO:0031647; P:regulation of protein stability; ISS:UniProtKB.
DR   GO; GO:0034976; P:response to endoplasmic reticulum stress; ISS:UniProtKB.
DR   GO; GO:0061709; P:reticulophagy; ISS:UniProtKB.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR019153; DDRGK_dom-contain.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF09756; DDRGK; 1.
DR   SMART; SM01128; DDRGK; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Membrane; Reference proteome; Signal;
KW   Ubl conjugation pathway.
FT   SIGNAL          1..25
FT                   /evidence="ECO:0000255"
FT   CHAIN           26..300
FT                   /note="DDRGK domain-containing protein 1"
FT                   /id="PRO_0000391852"
FT   DOMAIN          217..261
FT                   /note="PCI"
FT   REGION          28..173
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        92..173
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   300 AA;  34431 MW;  21FCB7304298DA2C CRC64;
     MDVVLYIAAA AILLVLIVFS VKIRGRTQDA DVEDHQNVTA RVSARPQAAP ERAAGMPRRR
     RGLHSRVNAQ RAQRASDNED SPVEADEDEE GRNASEERPQ AAGKVGAKKQ RKLEEKQARK
     AQREAEQEER EERKRLQELR DQERQKEEEK ERQQEQKQEE ELQRVKEEQE RREEEEYQRL
     KESFIIEDQG EAEELTEHES QSLLQEFIQY VQKSKVVLLE DLASQFGLRT QDAIARLQDL
     IADGSLTGVI DDRGKFIFIT PEELNAVAQF IKQRGRVSIS ELAQASNTLI NLTPDIHSSA