DDX18_DROME
ID DDX18_DROME Reviewed; 680 AA.
AC Q9VD51; A4V384; O77001; Q8SYP5;
DT 21-FEB-2001, integrated into UniProtKB/Swiss-Prot.
DT 15-AUG-2003, sequence version 2.
DT 03-AUG-2022, entry version 174.
DE RecName: Full=Probable ATP-dependent RNA helicase pitchoune;
DE EC=3.6.4.13;
GN Name=pit; ORFNames=CG6375;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND SUBCELLULAR LOCATION.
RC STRAIN=Oregon-R;
RX PubMed=9716523; DOI=10.1242/dev.125.18.3571;
RA Zaffran S., Chartier A., Gallant P., Astier M., Arquier N., Doherty D.,
RA Gratecos D., Semeriva M.;
RT "A Drosophila RNA helicase gene, pitchoune, is required for cell growth and
RT proliferation and is a potential target of d-Myc.";
RL Development 125:3571-3584(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Probable RNA-dependent helicase. Functions in cell growth and
CC proliferation. May have a role in ribosome biogenesis and,
CC consequently, in protein biosynthesis. {ECO:0000269|PubMed:9716523}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:9716523}.
CC -!- MISCELLANEOUS: Mutation in the pit gene produce larvae that cannot grow
CC beyond the first instar larval stage although they can live as long as
CC 7-10 days. All the tissues are equally affected and the perfectly
CC shaped larvae are indistinguishable from first instar wild-type
CC animals.
CC -!- MISCELLANEOUS: 'Pitchoune' means 'small' in Provence (Southern part of
CC France).
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX18/HAS1
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC27683.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; U84552; AAC27683.1; ALT_FRAME; mRNA.
DR EMBL; AE014297; AAF55951.2; -; Genomic_DNA.
DR EMBL; AE014297; AAN13900.2; -; Genomic_DNA.
DR EMBL; AY071402; AAL49024.1; -; mRNA.
DR EMBL; AY119620; AAM50274.1; -; mRNA.
DR RefSeq; NP_001287459.1; NM_001300530.1.
DR RefSeq; NP_524446.3; NM_079722.4.
DR RefSeq; NP_732694.2; NM_169991.2.
DR AlphaFoldDB; Q9VD51; -.
DR SMR; Q9VD51; -.
DR BioGRID; 67556; 26.
DR DIP; DIP-19700N; -.
DR IntAct; Q9VD51; 6.
DR STRING; 7227.FBpp0083572; -.
DR PaxDb; Q9VD51; -.
DR PRIDE; Q9VD51; -.
DR DNASU; 42595; -.
DR EnsemblMetazoa; FBtr0084174; FBpp0083572; FBgn0266581.
DR EnsemblMetazoa; FBtr0084175; FBpp0083573; FBgn0266581.
DR EnsemblMetazoa; FBtr0346345; FBpp0312062; FBgn0266581.
DR GeneID; 42595; -.
DR KEGG; dme:Dmel_CG6375; -.
DR UCSC; CG6375-RA; d. melanogaster.
DR UCSC; CG6375-RB; d. melanogaster.
DR CTD; 42595; -.
DR FlyBase; FBgn0266581; pit.
DR VEuPathDB; VectorBase:FBgn0266581; -.
DR eggNOG; KOG0342; Eukaryota.
DR GeneTree; ENSGT00680000100037; -.
DR HOGENOM; CLU_003041_26_5_1; -.
DR InParanoid; Q9VD51; -.
DR OMA; EYEFPAN; -.
DR OrthoDB; 973872at2759; -.
DR PhylomeDB; Q9VD51; -.
DR SignaLink; Q9VD51; -.
DR BioGRID-ORCS; 42595; 1 hit in 1 CRISPR screen.
DR ChiTaRS; pit; fly.
DR GenomeRNAi; 42595; -.
DR PRO; PR:Q9VD51; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0266581; Expressed in cleaving embryo and 56 other tissues.
DR ExpressionAtlas; Q9VD51; baseline and differential.
DR Genevisible; Q9VD51; DM.
DR GO; GO:0005730; C:nucleolus; IDA:FlyBase.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003724; F:RNA helicase activity; ISS:FlyBase.
DR GO; GO:0000463; P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IBA:GO_Central.
DR CDD; cd17942; DEADc_DDX18; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR044773; DDX18/Has1_DEADc.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR025313; DUF4217.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF13959; DUF4217; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM01178; DUF4217; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Nucleus;
KW Reference proteome; RNA-binding.
FT CHAIN 1..680
FT /note="Probable ATP-dependent RNA helicase pitchoune"
FT /id="PRO_0000055003"
FT DOMAIN 218..393
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 407..577
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 1..168
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 659..680
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 187..215
FT /note="Q motif"
FT MOTIF 341..344
FT /note="DEVD box"
FT COMPBIAS 1..32
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 40..61
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 94..138
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 231..238
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT CONFLICT 30
FT /note="N -> K (in Ref. 1; AAC27683)"
FT /evidence="ECO:0000305"
FT CONFLICT 115
FT /note="V -> A (in Ref. 1; AAC27683)"
FT /evidence="ECO:0000305"
FT CONFLICT 512..517
FT /note="DDPREY -> GDQASI (in Ref. 1; AAC27683)"
FT /evidence="ECO:0000305"
FT CONFLICT 634..644
FT /note="GAAKRERPEKR -> ARPSGSDRKSD (in Ref. 1; AAC27683)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 680 AA; 76928 MW; E71CD18B8A135FF1 CRC64;
MSIREKLLMK KIVKREKMKK ELSQKKGNKN AQKQEPPKQN GNKPSKKPEK LSKKHVAKDE
DDDLEEDFQE APLPKKKQQK QPPKKQQIQV ANSDSESDDD EQEDEADEDS DLDEVAEVDE
EDVDSGSEDD DQQEDEDEEE PVPAKKTKLL PNKSKAQNGK PAKDDEPFTV ESSLAALDYR
DSDDRSFASL KGAVSEATLR AIKEMGFTEM TEIQSKSLTP LLKGRDLVGA AQTGSGKTLA
FLIPAVELIN KLRFMPRNGT GVIIISPTRE LSMQTFGVLK ELMAHHHHTY GLVMGGSNRQ
VESEKLGKGI NILVATPGRL LDHLQNSPDF LYKNLQCLII DEVDRILEIG FEEELKQIIN
LLPKRRQTML FSATQTARIE ALSKLALKSE PIYVGVHDNQ DTATVDGLEQ GYIVCPSEKR
LLVLFTFLKK NRKKKVMVFF SSCMSVKYHH ELFNYIDLPV TSIHGKQKQT KRTTTFFQFC
NAESGILLCT DVAARGLDIP QVDWIVQYDP PDDPREYIHR VGRTARGSGT SGHALLLMRP
EELGFLRYLK AAKVPLNEFE FSWQKIADIQ LQLEKLIAKN YFLNQSAKEA FKSYVRAYDS
HQLKQIFNVN TLDLQAVAKS FGFLVPPVVD LKVGAAKRER PEKRVGGGGF GFYKKMNEGS
ASKQRHFKQV NRDQAKKFMR
