DDX19_DICDI
ID DDX19_DICDI Reviewed; 465 AA.
AC Q54TF8; O00898;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=ATP-dependent RNA helicase ddx19;
DE EC=3.6.4.13;
DE AltName: Full=ATP-dependent RNA helicase helC;
DE AltName: Full=DEAD box protein 19;
GN Name=helC; Synonyms=ddx19; ORFNames=DDB_G0281553;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC STRAIN=AX3;
RX PubMed=9601648; DOI=10.1016/s0960-9822(98)70233-7;
RA Machesky L.M., Insall R.H., Kay R.R.;
RT "The helC gene encodes a putative DEAD-box RNA helicase required for
RT development in Dictyostelium discoideum.";
RL Curr. Biol. 8:607-610(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: ATP-binding RNA helicase required for normal differentiation
CC and development. {ECO:0000269|PubMed:9601648}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC family of RNA helicases and controls ATP binding and hydrolysis.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX19/DBP5
CC subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB60938.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF002677; AAB60938.1; ALT_FRAME; mRNA.
DR EMBL; AAFI02000042; EAL66538.1; -; Genomic_DNA.
DR RefSeq; XP_640633.1; XM_635541.1.
DR AlphaFoldDB; Q54TF8; -.
DR SMR; Q54TF8; -.
DR STRING; 44689.DDB0215338; -.
DR PaxDb; Q54TF8; -.
DR PRIDE; Q54TF8; -.
DR EnsemblProtists; EAL66538; EAL66538; DDB_G0281553.
DR GeneID; 8623244; -.
DR KEGG; ddi:DDB_G0281553; -.
DR dictyBase; DDB_G0281553; helC.
DR eggNOG; KOG0332; Eukaryota.
DR HOGENOM; CLU_003041_1_0_1; -.
DR InParanoid; Q54TF8; -.
DR OMA; DFKNLCM; -.
DR PhylomeDB; Q54TF8; -.
DR PRO; PR:Q54TF8; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0010494; C:cytoplasmic stress granule; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0003724; F:RNA helicase activity; IBA:GO_Central.
DR GO; GO:0016973; P:poly(A)+ mRNA export from nucleus; IBA:GO_Central.
DR GO; GO:0010628; P:positive regulation of gene expression; IMP:dictyBase.
DR GO; GO:0030587; P:sorocarp development; IMP:dictyBase.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Developmental protein; Helicase; Hydrolase;
KW Nucleotide-binding; Reference proteome; RNA-binding.
FT CHAIN 1..465
FT /note="ATP-dependent RNA helicase ddx19"
FT /id="PRO_0000327407"
FT DOMAIN 102..268
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 297..449
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 1..45
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 70..98
FT /note="Q motif"
FT MOTIF 215..218
FT /note="DEAD box"
FT COMPBIAS 1..22
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 115..122
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT CONFLICT 52..53
FT /note="GI -> EF (in Ref. 1; AAB60938)"
FT /evidence="ECO:0000305"
FT CONFLICT 69
FT /note="V -> W (in Ref. 1; AAB60938)"
FT /evidence="ECO:0000305"
FT CONFLICT 100
FT /note="A -> T (in Ref. 1; AAB60938)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 465 AA; 51720 MW; B6756CA7D0B8B689 CRC64;
MSEKETNTTS TENKEKEKQE QTNTNSTTES TNNQVDEEYE RPGRSEGLDE FGIQLDIQQS
DPNSPLYSVK TFEELGLKPE LLKGVYAMGY NKPSKIQEAA LPIIIQSPNN LIAQSQSGTG
KTAAFTLGML NCVDPSINAP QAICISPTKE LALQTFEVIS KIGQFSNIKP LLYISEIEVP
KNVTNQVIIG TPGKILENVI KKQLSVKFLK MVVLDEADFI VKMKNVPNQI AMINRLLPSN
VKVCLFSATF SMGVEELIKK IVQDPYTSIR LKRQELSVEK IHQYFIDCGS EDNKALILSD
IYGFISVGQS IVFVHTIATA KSVHQKMVDE GHSVSLLYGK DLTTEERFKQ IKDFKDGKSK
VLITTNVLAR GIDIPQVSLV INYDVPLDEM GKPDPVHYLH RIGRVGRFGR SGVALSFVYD
QQSTNKLMNI STHLGVPLKE LKSSEIESLD GILKGIRNQL TPLNN
