DDX1_DROME
ID DDX1_DROME Reviewed; 727 AA.
AC Q9VNV3; O61663; Q24131;
DT 15-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=ATP-dependent RNA helicase Ddx1;
DE Short=DEAD box protein 1;
DE EC=3.6.4.13;
GN Name=Ddx1; ORFNames=CG9054;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND DEVELOPMENTAL STAGE.
RC TISSUE=Embryo;
RX PubMed=8666277; DOI=10.1016/0378-1119(96)00034-0;
RA Rafti F., Scarvelis D., Lasko P.F.;
RT "A Drosophila melanogaster homologue of the human DEAD-box gene DDX1.";
RL Gene 171:225-229(1996).
RN [2] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5] {ECO:0000305, ECO:0000312|EMBL:AAM50315.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 458-727.
RC STRAIN=Berlin;
RA Zinsmaier K.E., Eberle K.K., Buchner E.;
RL Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Acts as an ATP-dependent RNA helicase, able to unwind both
CC RNA-RNA and RNA-DNA duplexes. Possesses 5' single-stranded RNA overhang
CC nuclease activity (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically
CC throughout development. Expression is highest in early embryos.
CC {ECO:0000269|PubMed:8666277}.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX1 subfamily.
CC {ECO:0000305}.
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DR EMBL; U34773; AAC47309.1; -; mRNA.
DR EMBL; AE014296; AAF51814.1; -; Genomic_DNA.
DR EMBL; AY119661; AAM50315.1; -; mRNA.
DR EMBL; AF057167; AAD09429.1; -; Genomic_DNA.
DR RefSeq; NP_524212.2; NM_079488.4.
DR AlphaFoldDB; Q9VNV3; -.
DR SMR; Q9VNV3; -.
DR BioGRID; 65694; 4.
DR IntAct; Q9VNV3; 1.
DR STRING; 7227.FBpp0078144; -.
DR PaxDb; Q9VNV3; -.
DR PRIDE; Q9VNV3; -.
DR DNASU; 40457; -.
DR EnsemblMetazoa; FBtr0078492; FBpp0078144; FBgn0015075.
DR GeneID; 40457; -.
DR KEGG; dme:Dmel_CG9054; -.
DR UCSC; CG9054-RA; d. melanogaster.
DR CTD; 1653; -.
DR FlyBase; FBgn0015075; Ddx1.
DR VEuPathDB; VectorBase:FBgn0015075; -.
DR eggNOG; KOG0349; Eukaryota.
DR GeneTree; ENSGT00940000155678; -.
DR HOGENOM; CLU_016321_0_0_1; -.
DR InParanoid; Q9VNV3; -.
DR OMA; EYCIRAI; -.
DR OrthoDB; 973872at2759; -.
DR PhylomeDB; Q9VNV3; -.
DR SignaLink; Q9VNV3; -.
DR BioGRID-ORCS; 40457; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 40457; -.
DR PRO; PR:Q9VNV3; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0015075; Expressed in embryonic/larval hemocyte (Drosophila) and 23 other tissues.
DR Genevisible; Q9VNV3; DM.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003682; F:chromatin binding; ISS:UniProtKB.
DR GO; GO:0033677; F:DNA/RNA helicase activity; ISS:UniProtKB.
DR GO; GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0004518; F:nuclease activity; ISS:UniProtKB.
DR GO; GO:0008143; F:poly(A) binding; ISS:UniProtKB.
DR GO; GO:0003724; F:RNA helicase activity; ISS:UniProtKB.
DR GO; GO:0003712; F:transcription coregulator activity; ISS:UniProtKB.
DR GO; GO:0032508; P:DNA duplex unwinding; ISS:UniProtKB.
DR GO; GO:0006302; P:double-strand break repair; ISS:UniProtKB.
DR GO; GO:0001700; P:embryonic development via the syncytial blastoderm; IEP:UniProtKB.
DR GO; GO:0048477; P:oogenesis; IMP:FlyBase.
DR GO; GO:0007283; P:spermatogenesis; IMP:FlyBase.
DR GO; GO:0008033; P:tRNA processing; IMP:FlyBase.
DR Gene3D; 2.60.120.920; -; 1.
DR Gene3D; 3.40.50.300; -; 3.
DR InterPro; IPR001870; B30.2/SPRY.
DR InterPro; IPR043136; B30.2/SPRY_sf.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR003877; SPRY_dom.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF00622; SPRY; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00449; SPRY; 1.
DR SUPFAM; SSF49899; SSF49899; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
DR PROSITE; PS50188; B302_SPRY; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 2.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Exonuclease; Helicase; Hydrolase; Nuclease;
KW Nucleotide-binding; Reference proteome; RNA-binding.
FT CHAIN 1..727
FT /note="ATP-dependent RNA helicase Ddx1"
FT /id="PRO_0000054989"
FT DOMAIN 2..428
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 69..246
FT /note="B30.2/SPRY"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00548"
FT DOMAIN 483..676
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT MOTIF 370..373
FT /note="DEAD box"
FT BINDING 46..53
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT CONFLICT 78..89
FT /note="KGGAIGGAVTPW -> QGRCNWRSCDSV (in Ref. 1; AAC47309)"
FT /evidence="ECO:0000305"
FT CONFLICT 217
FT /note="L -> S (in Ref. 1; AAC47309)"
FT /evidence="ECO:0000305"
FT CONFLICT 274..279
FT /note="AAGAPS -> DSWST (in Ref. 1; AAC47309)"
FT /evidence="ECO:0000305"
FT CONFLICT 365
FT /note="R -> G (in Ref. 1; AAC47309)"
FT /evidence="ECO:0000305"
FT CONFLICT 429
FT /note="V -> G (in Ref. 1; AAC47309)"
FT /evidence="ECO:0000305"
FT CONFLICT 458
FT /note="S -> C (in Ref. 4; AAD09429)"
FT /evidence="ECO:0000305"
FT CONFLICT 529..530
FT /note="GG -> D (in Ref. 1; AAC47309)"
FT /evidence="ECO:0000305"
FT CONFLICT 580..589
FT /note="MINVTLPDDK -> SKFSNFETIT (in Ref. 4; AAD09429)"
FT /evidence="ECO:0000305"
FT CONFLICT 682
FT /note="K -> P (in Ref. 1; AAC47309)"
FT /evidence="ECO:0000305"
FT CONFLICT 704
FT /note="V -> E (in Ref. 1; AAC47309)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 727 AA; 80869 MW; 52B7BE91A33599DB CRC64;
MTAFEEFGVL PELGMATDEL DWTLPTDVQA EAIPLILGGG DVLMAAETGS GKTGAFCLPI
LQIVWETLRD LEEGKAGKGG AIGGAVTPWT MSFFDRGNAL AVTPDGLRCQ SREFKEWHGC
RATTGVRGKG KFYFEATVTD EGLCRVGWST QQANLDLGTC RMGFGFGGTG KKSNNRQFDD
YGEAFGKADV IGCLLDLKNQ EVSFTKNGQN LGVAFRLPDN LAKETFYPAV VLKNAEMQFN
FGKTDFKYAP GNGFVGACQA GPEHSKANPI TGPAAGAPSA KPAPNAPQAI IMEPSRELAE
QTYNQIEKFK YHLSNPEVRS LLLIGGVRLE EQKAQLMQGT HIVVGTPGRL EEMINSGLVL
LTHCRFFVLD EADALLKQGY TELIDRLHKQ IPKITSDGRR LQMVVCSATL HAFEVKKMAE
RLMHFPTWVD LKGEDAVPET VHHVVCLVDP QMDTTWQSLR QPIGTDGVHD RDNVHPGNHS
KETLSQAVKL LKGEYCVHAI DKHNMDRAII FCRTKQDCDN LERFLRQRGG KHYSCVCLHG
DRKPQERKEN LEMFKRQQVK FLICTDVAAR GLDITGLPFM INVTLPDDKT NYVHRIGRVG
RAERMGLAIS LVATVPEKVW YHGEWCKSRG RSCNNTNLTE VRGCCIWYNE PNLLAEVEDH
LNITIQQVDK TMDVPVNDFD GKVVYGQKNL RTGSGYEDHV EQLVPTVRKL TELELQSQSL
FLKRLKV
