DDX23_DICDI
ID DDX23_DICDI Reviewed; 834 AA.
AC Q54Y81; Q23910;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=ATP-dependent RNA helicase ddx23;
DE EC=3.6.4.13;
DE AltName: Full=ATP-dependent RNA helicase helB2;
DE AltName: Full=DEAD box protein 23;
GN Name=helB2; Synonyms=ddx23, hel2B; ORFNames=DDB_G0277857;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 272-834.
RC STRAIN=AX2;
RX PubMed=7695838; DOI=10.1515/bchm3.1994.375.11.759;
RA Mahal B., Nellen W.;
RT "Developmental regulation of DEAD box proteins and cloning of putative RNA
RT helicase genes from Dictyostelium discoideum.";
RL Biol. Chem. Hoppe-Seyler 375:759-763(1994).
CC -!- FUNCTION: Probable ATP-dependent RNA helicase which may be involved in
CC mRNA splicing. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC family of RNA helicases and controls ATP binding and hydrolysis.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX23/PRP28
CC subfamily. {ECO:0000305}.
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DR EMBL; AAFI02000023; EAL68101.1; -; Genomic_DNA.
DR EMBL; X81824; CAA57418.1; -; mRNA.
DR RefSeq; XP_642321.1; XM_637229.1.
DR AlphaFoldDB; Q54Y81; -.
DR SMR; Q54Y81; -.
DR STRING; 44689.DDB0219950; -.
DR PaxDb; Q54Y81; -.
DR PRIDE; Q54Y81; -.
DR EnsemblProtists; EAL68101; EAL68101; DDB_G0277857.
DR GeneID; 8621527; -.
DR KEGG; ddi:DDB_G0277857; -.
DR dictyBase; DDB_G0277857; helB2.
DR eggNOG; KOG0333; Eukaryota.
DR HOGENOM; CLU_003041_11_2_1; -.
DR InParanoid; Q54Y81; -.
DR OMA; YLVSTEM; -.
DR PhylomeDB; Q54Y81; -.
DR Reactome; R-DDI-72163; mRNA Splicing - Major Pathway.
DR Reactome; R-DDI-72165; mRNA Splicing - Minor Pathway.
DR PRO; PR:Q54Y81; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; IBA:GO_Central.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Cytoplasm; Helicase; Hydrolase; mRNA processing;
KW mRNA splicing; Nucleotide-binding; Nucleus; Reference proteome.
FT CHAIN 1..834
FT /note="ATP-dependent RNA helicase ddx23"
FT /id="PRO_0000327433"
FT DOMAIN 444..643
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 654..815
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 1..245
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 322..371
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 813..834
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 413..441
FT /note="Q motif"
FT MOTIF 570..573
FT /note="DEAD box"
FT COMPBIAS 1..26
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 38..57
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 67..202
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 215..245
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 323..368
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 457..464
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 834 AA; 95823 MW; D333AD48BE893C48 CRC64;
MDPPKLTFIS KRDTKKKDEV NKEQPTKNLK ILDLFSNDEE FSNPTQEEPT NTLQEKLMNV
DPLEFFSKGG LKEEQKKERD DHRDDYRDSR DRDRDYRDNG GRDRDRDYRD GGGGGGGRDR
DRNRDRDRDR DRDYRDGGGG RDRYRDNDRY RDTDRYRDND RRDGSGSGSS RRRDERRENS
GRRDYRDNDR RDDRRDNGRY GRDNDNSGGG GSGKNSSDKK EEINPVSNNN DIHKDRIKRD
TTQFSHKVFE QINNKRDRED PELRDIKVDY MGIKRDENRK KIKGEKGKFV FEWDSSEDTS
SDYNTLYTKK LEIQPQFGHG NFGGYEKNNN NNGNHYNGNI YNNNNNNNNN NNNNNNINNN
NNGSMIGGKQ ISELPDTHWS KKPLKSMTKR DWHIFKEDFN ISTKGGIAPN PIRTWQESNL
PREILEAIRQ LGYEKPSPIQ MQSIPISLTG RDILGIAETG SGKTCAFVIP MLIYISKQPR
LTKDTEADGP YALVMAPTRE LVQQIEKETR NFAQHFGFRV VSLVGGQSIE DQAYQVSKGC
EIIIATPGRL NDCLEKRYLV LNQCNYIVLD EADMMIDLGF EPQVTSVLDA MPSSFLKSED
DEMAEKQESD RSHIYRTTIL FSATMPPLVE KLSKKYLRRP CTITIGEAGK VVDRIRQTVI
FVKSENDKKE HLTQLIKDGP PPPIIIFVNK KKHCDIIAPV LEECRVSYTI LHSGRSQEQR
EAALEGFKKR KYEVLIATGV ASRGIHVDGV THVINFDIPK NIEDYTHRIG RTGRAGSAGL
ASSFITDKDV EIMYDLKQIL TSTNNIVPIE LLKHPSSQQK HGSSKDHNKS VIFK
