DDX42_DICDI
ID DDX42_DICDI Reviewed; 986 AA.
AC Q54IV3;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Probable ATP-dependent RNA helicase ddx42;
DE EC=3.6.4.13;
DE AltName: Full=DEAD box protein 42;
GN Name=ddx42; ORFNames=DDB_G0288501;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: probable ATP-dependent RNA helicase which may bind to
CC partially double-stranded RNAs (dsRNAs) in order to unwind RNA
CC secondary structures. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC family of RNA helicases and controls ATP binding and hydrolysis.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX42 subfamily.
CC {ECO:0000305}.
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DR EMBL; AAFI02000112; EAL63199.1; -; Genomic_DNA.
DR RefSeq; XP_636700.1; XM_631608.1.
DR AlphaFoldDB; Q54IV3; -.
DR SMR; Q54IV3; -.
DR STRING; 44689.DDB0233432; -.
DR PaxDb; Q54IV3; -.
DR EnsemblProtists; EAL63199; EAL63199; DDB_G0288501.
DR GeneID; 8626657; -.
DR KEGG; ddi:DDB_G0288501; -.
DR dictyBase; DDB_G0288501; ddx42.
DR eggNOG; KOG0339; Eukaryota.
DR HOGENOM; CLU_003041_9_0_1; -.
DR InParanoid; Q54IV3; -.
DR OMA; WTVPQNI; -.
DR PhylomeDB; Q54IV3; -.
DR Reactome; R-DDI-72163; mRNA Splicing - Major Pathway.
DR Reactome; R-DDI-72165; mRNA Splicing - Minor Pathway.
DR PRO; PR:Q54IV3; -.
DR Proteomes; UP000002195; Chromosome 5.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
DR PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 3: Inferred from homology;
KW ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Nucleus;
KW Reference proteome; RNA-binding.
FT CHAIN 1..986
FT /note="Probable ATP-dependent RNA helicase ddx42"
FT /id="PRO_0000327413"
FT DOMAIN 336..511
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 522..684
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 1..149
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 165..192
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 206..252
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 688..986
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 305..333
FT /note="Q motif"
FT MOTIF 459..462
FT /note="DEAD box"
FT COMPBIAS 23..85
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 104..139
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 165..180
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 721..986
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 349..356
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 986 AA; 110817 MW; D7ACC579FBB9FFB6 CRC64;
MSKRVSAFGA EGDDEPQEKP MKPFISFSSN INNNNNSNNN NNNNNNNNNN NNNNNNKNNI
GTGINLNIKN NNNINNNNNK SGFPVKKSRF NGEDDDDDYF FSNVPPKSSM TTLNKSPPNF
ENASSNNNNN NNNNNQESDS KNQNEDEDDE IDPLDAFMEN VNAQAAIDNS KSIEKGQQQQ
QSLKSKRDDI DNEDDEEIFY KLRQKQLANK SSKQQQDDDV DYSSLDDDDG YFDDEESLKN
GQSKGKRIIE PLPPIDHSKE EYIEFNKIFY EEHPDIANLT EEQVFEIRKN LDIRMTGTDL
INPVTSFGHY GFDDILLQAI AKQSIETPTP IQKQAIPIAL SGRDLIAIAK TGSGKTATFI
WPSISHIMDQ PYLEKGDGPI ALFLAPTREL AHQIYLETLK YSKYFKLKTT VLYGGVSKQQ
QCKELKAGCE IIVATPGRLI DMIKLKATKL NRVSYLVLDE ADKMFDFGFG PQVLSIVNHV
RPDRQTLLFS ATFKPNVEEF ARTILSDPIK ISIGMIGSAN SDITQIVQVL KSDSDKWNWL
TNQLALLLSQ GSVLIFVSTK VAVEQLSSNL TKFGFQTCTL HGDKNQIERS QTIQTFKEGK
INILIATDVA ARGLDIPLIK NVVNYDTSRD IESHTHRIGR TGRAGNTGVA YTLITPKDIH
FSVDLIKNLE SASQFVPPEL IDVAMNNPHF KRERGGGGGG SNRGRGRGGG GVGYRRNSRG
GGVAFNSNRD SSRSDSQNKF IPAQSVEGGR LFNPNNTDNS EINNENEKSI NNENKFSNNN
SGSSNDRNSI NYRNNSFNNN SNNTNNSGNS NFNNSNSNNG YSNNNYNNNY KNNSNYNNSN
NNNNSYYNNN NSNNNNNSNY NNSSNNNNNN NNNYRNGNNN NNYNNNNYYN NNNSNNNSSN
NNNSNNNSSN NNFNNNFNNN NNNNDNSNFN RALPFNDFNN NNNNSNNNNF NYNNNFNNSY
NANNSNHYKN NNNSNNFNQR SQYNRR
