DDX49_DROME
ID DDX49_DROME Reviewed; 521 AA.
AC Q07886; Q9V528;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 169.
DE RecName: Full=Probable ATP-dependent RNA helicase Dbp45A;
DE Short=DEAD box protein 45A;
DE EC=3.6.4.13;
GN Name=Dbp45A; ORFNames=CG12759;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE.
RX PubMed=7692973; DOI=10.1016/0167-4781(93)90052-f;
RA Lavoie C.A., Harvey M., Lasko P.F.;
RT "Dbp45A encodes a Drosophila DEAD box protein with similarity to a putative
RT yeast helicase involved in ribosome assembly.";
RL Biochim. Biophys. Acta 1216:140-144(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Probable ATP-binding RNA helicase.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX49/DBP8
CC subfamily. {ECO:0000305}.
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DR EMBL; L13612; AAA16339.1; -; Unassigned_DNA.
DR EMBL; Z23266; CAA80804.1; -; Genomic_DNA.
DR EMBL; AE013599; AAF58994.1; -; Genomic_DNA.
DR EMBL; AY058728; AAL13957.1; -; mRNA.
DR PIR; S38329; S38329.
DR RefSeq; NP_476927.1; NM_057579.5.
DR AlphaFoldDB; Q07886; -.
DR SMR; Q07886; -.
DR IntAct; Q07886; 3.
DR STRING; 7227.FBpp0087645; -.
DR PaxDb; Q07886; -.
DR PRIDE; Q07886; -.
DR DNASU; 35917; -.
DR EnsemblMetazoa; FBtr0088564; FBpp0087645; FBgn0010220.
DR GeneID; 35917; -.
DR KEGG; dme:Dmel_CG12759; -.
DR CTD; 35917; -.
DR FlyBase; FBgn0010220; Dbp45A.
DR VEuPathDB; VectorBase:FBgn0010220; -.
DR eggNOG; KOG0340; Eukaryota.
DR GeneTree; ENSGT00730000111231; -.
DR HOGENOM; CLU_003041_1_1_1; -.
DR InParanoid; Q07886; -.
DR OMA; EIKQESM; -.
DR OrthoDB; 744428at2759; -.
DR PhylomeDB; Q07886; -.
DR Reactome; R-DME-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR BioGRID-ORCS; 35917; 1 hit in 1 CRISPR screen.
DR GenomeRNAi; 35917; -.
DR PRO; PR:Q07886; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0010220; Expressed in embryonic/larval hemocyte (Drosophila) and 30 other tissues.
DR Genevisible; Q07886; DM.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003724; F:RNA helicase activity; ISS:FlyBase.
DR GO; GO:0000381; P:regulation of alternative mRNA splicing, via spliceosome; HMP:FlyBase.
DR GO; GO:0006364; P:rRNA processing; IBA:GO_Central.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Reference proteome;
KW RNA-binding.
FT CHAIN 1..521
FT /note="Probable ATP-dependent RNA helicase Dbp45A"
FT /id="PRO_0000055004"
FT DOMAIN 38..209
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 237..386
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 448..521
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 7..35
FT /note="Q motif"
FT MOTIF 157..160
FT /note="DEAD box"
FT COMPBIAS 449..521
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 51..58
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT CONFLICT 161
FT /note="R -> P (in Ref. 1; AAA16339/CAA80804)"
FT /evidence="ECO:0000305"
FT CONFLICT 211..217
FT /note="SQDSDVA -> CEASAVR (in Ref. 1; AAA16339)"
FT /evidence="ECO:0000305"
FT CONFLICT 224..225
FT /note="QR -> HG (in Ref. 1; AAA16339/CAA80804)"
FT /evidence="ECO:0000305"
FT CONFLICT 228..229
FT /note="LC -> VS (in Ref. 1; AAA16339/CAA80804)"
FT /evidence="ECO:0000305"
FT CONFLICT 234
FT /note="R -> P (in Ref. 1; AAA16339/CAA80804)"
FT /evidence="ECO:0000305"
FT CONFLICT 327
FT /note="M -> I (in Ref. 1; AAA16339/CAA80804)"
FT /evidence="ECO:0000305"
FT CONFLICT 407
FT /note="D -> C (in Ref. 1; AAA16339/CAA80804)"
FT /evidence="ECO:0000305"
FT CONFLICT 456
FT /note="P -> S (in Ref. 1; AAA16339/CAA80804)"
FT /evidence="ECO:0000305"
FT CONFLICT 493
FT /note="D -> Y (in Ref. 1; AAA16339/CAA80804)"
FT /evidence="ECO:0000305"
FT CONFLICT 517..521
FT /note="KKDKA -> NKSKGKIKRDV (in Ref. 1; AAA16339)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 521 AA; 59678 MW; 89C68C43CD947913 CRC64;
MQRKEANPFQ ILGLRPWLVK QLTKLGLKGA TPIQQKCIPA ILAGQDCIGA AKTGSGKTFA
FALPILERLS EEPVSHFALV LTPTHELAYQ ISEQFLVAGQ AMGVRVCVVS GGTDQMVESQ
KLMQRPHIVV AMPGRLADHL TGCDTFSFDN LKYLVVDEAD RMLNGDFDES LSIIERCLPK
TRQNLFFSAT MKDFIKESSI FPIASDCFEW SQDSDVATVE TLDQRYLLCA DYDRDMVLIE
ALRKYREENE NANVMIFTNT KKYCQLLSMT LKNMEIDNVC LHGFMRQKER VAALSRFKSN
QIRTLIATDV AARGLDIPSV ELVMNHMLPR TPKEYIHRVG RTARAGRKGM SISIFRFPRD
LELLAAIEEE INTKLTEHPI DQRMVERIFM QVNVTRRESE MQLDNNDFDE RAQNYRRKTW
IMEGKDPDQM EALYRKKQKD KLREIRRKRK LQHAEPAASE EGKALLQDER FKSVDSARFE
KKGKGRSRAT QEDTPTKPLK RLNKEKPVAQ KGRADVKKDK A
