DDX52_MOUSE
ID DDX52_MOUSE Reviewed; 598 AA.
AC Q8K301; Q3UZY9; Q8BV29;
DT 09-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Probable ATP-dependent RNA helicase DDX52;
DE EC=3.6.4.13;
DE AltName: Full=ATP-dependent RNA helicase ROK1-like;
DE AltName: Full=DEAD box protein 52;
GN Name=Ddx52; Synonyms=Rok1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Retina, and Xiphoid cartilage;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX52/ROK1
CC subfamily. {ECO:0000305}.
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DR EMBL; AK080767; BAC38014.1; -; mRNA.
DR EMBL; AK133545; BAE21716.1; -; mRNA.
DR EMBL; AL645615; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466556; EDL15728.1; -; Genomic_DNA.
DR EMBL; BC029094; AAH29094.1; -; mRNA.
DR CCDS; CCDS25180.1; -.
DR RefSeq; NP_084372.2; NM_030096.2.
DR AlphaFoldDB; Q8K301; -.
DR SMR; Q8K301; -.
DR BioGRID; 219368; 1.
DR STRING; 10090.ENSMUSP00000048802; -.
DR iPTMnet; Q8K301; -.
DR PhosphoSitePlus; Q8K301; -.
DR EPD; Q8K301; -.
DR MaxQB; Q8K301; -.
DR PaxDb; Q8K301; -.
DR PRIDE; Q8K301; -.
DR ProteomicsDB; 279905; -.
DR Antibodypedia; 74465; 117 antibodies from 23 providers.
DR DNASU; 78394; -.
DR Ensembl; ENSMUST00000049257; ENSMUSP00000048802; ENSMUSG00000020677.
DR GeneID; 78394; -.
DR KEGG; mmu:78394; -.
DR UCSC; uc007kpz.2; mouse.
DR CTD; 11056; -.
DR MGI; MGI:1925644; Ddx52.
DR VEuPathDB; HostDB:ENSMUSG00000020677; -.
DR eggNOG; KOG0344; Eukaryota.
DR GeneTree; ENSGT00550000074863; -.
DR HOGENOM; CLU_003041_1_4_1; -.
DR InParanoid; Q8K301; -.
DR OMA; DRALMAC; -.
DR OrthoDB; 400908at2759; -.
DR PhylomeDB; Q8K301; -.
DR TreeFam; TF314448; -.
DR Reactome; R-MMU-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR BioGRID-ORCS; 78394; 29 hits in 77 CRISPR screens.
DR ChiTaRS; Ddx52; mouse.
DR PRO; PR:Q8K301; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q8K301; protein.
DR Bgee; ENSMUSG00000020677; Expressed in undifferentiated genital tubercle and 262 other tissues.
DR Genevisible; Q8K301; MM.
DR GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0030490; P:maturation of SSU-rRNA; IBA:GO_Central.
DR CDD; cd17957; DEADc_DDX52; 1.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR044764; DDX52/Rok1_DEADc.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 2: Evidence at transcript level;
KW Acetylation; ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Nucleus;
KW Phosphoprotein; Reference proteome; RNA-binding.
FT CHAIN 1..598
FT /note="Probable ATP-dependent RNA helicase DDX52"
FT /id="PRO_0000055061"
FT DOMAIN 197..375
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 386..547
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT MOTIF 166..194
FT /note="Q motif"
FT MOTIF 319..322
FT /note="DEAD box"
FT BINDING 210..217
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT MOD_RES 15
FT /note="N6-acetyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y2R4"
FT MOD_RES 39
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9Y2R4"
FT CONFLICT 75
FT /note="Q -> E (in Ref. 4; AAH29094)"
FT /evidence="ECO:0000305"
FT CONFLICT 81
FT /note="R -> K (in Ref. 1; BAC38014)"
FT /evidence="ECO:0000305"
FT CONFLICT 103
FT /note="F -> L (in Ref. 4; AAH29094)"
FT /evidence="ECO:0000305"
FT CONFLICT 119
FT /note="D -> E (in Ref. 4; AAH29094)"
FT /evidence="ECO:0000305"
FT CONFLICT 312
FT /note="N -> S (in Ref. 4; AAH29094)"
FT /evidence="ECO:0000305"
FT CONFLICT 373
FT /note="V -> I (in Ref. 4; AAH29094)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 598 AA; 67474 MW; B4C89C522C4A79A9 CRC64;
MDSYDLFRRL GAGAKFDVKR FSADATRFQV GKRKFDSESL EVLKGLDFFG NKKSVSDECG
ALQIHQEPPN EEKTQGVLLE RSKEPKKKKR KKMTSEVPAQ EDFDGGIQWT SSVEAKLQDE
KVSGEKKLTS GKLEHLRKEK VNFFRNKHKI HVQGTDLPDP IATFQQLDQE YKINSRLLQN
ILDAGFQVPT PIQMQAIPVM LHGRELLASA PTGSGKTLAF SIPILMQLKQ PTNKGFRALV
ISPTRELASQ IHRELIKISE GTGFRIHMIH KAAIAAKKFG PKSSKKFDIL VTTPNRLIYL
LKQDPPGIDL TNVEWLVVDE SDKLFEDGKT GFREQLASIF LACTSPKVRR AMFSATFAYD
VEQWCKLNLD NVVSVSIGAR NSAVETVEQE LLFVGSETGK LLAMRELVKK GFKPPVLVFV
QSIERAKELF HELIYEGINV DVIHAERTQQ QRDNTVHSFR AGKIWVLICT ALLARGIDFK
GVNLVINYDF PTSSVEYIHR IGRTGRAGNR GKAVTFFTED DKPLLRSVAN VIQQAGCPVP
EYIKGFQKLL SKQKKKMIKK PLERESICTT PKYFLEQAKQ KKVAGQNSKK KETLKEKS
