DDX56_MOUSE
ID DDX56_MOUSE Reviewed; 546 AA.
AC Q9D0R4;
DT 23-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=Probable ATP-dependent RNA helicase DDX56;
DE EC=3.6.4.13;
DE AltName: Full=ATP-dependent 61 kDa nucleolar RNA helicase;
DE AltName: Full=DEAD box protein 56;
GN Name=Ddx56; Synonyms=D11Ertd619e, Noh61;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Salivary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: May play a role in later stages of the processing of the pre-
CC ribosomal particles leading to mature 60S ribosomal subunits. Has
CC intrinsic ATPase activity (By similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- SUBUNIT: May form homooligomeric complexes.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX56/DBP9
CC subfamily. {ECO:0000305}.
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DR EMBL; AK011136; BAB27426.1; -; mRNA.
DR EMBL; AK077514; BAC36839.1; -; mRNA.
DR EMBL; BC018291; AAH18291.1; -; mRNA.
DR CCDS; CCDS24414.1; -.
DR RefSeq; NP_080814.1; NM_026538.3.
DR AlphaFoldDB; Q9D0R4; -.
DR SMR; Q9D0R4; -.
DR BioGRID; 206632; 4.
DR CORUM; Q9D0R4; -.
DR IntAct; Q9D0R4; 1.
DR STRING; 10090.ENSMUSP00000004507; -.
DR iPTMnet; Q9D0R4; -.
DR PhosphoSitePlus; Q9D0R4; -.
DR EPD; Q9D0R4; -.
DR MaxQB; Q9D0R4; -.
DR PaxDb; Q9D0R4; -.
DR PRIDE; Q9D0R4; -.
DR ProteomicsDB; 279182; -.
DR Antibodypedia; 13419; 357 antibodies from 27 providers.
DR DNASU; 52513; -.
DR Ensembl; ENSMUST00000004507; ENSMUSP00000004507; ENSMUSG00000004393.
DR GeneID; 52513; -.
DR KEGG; mmu:52513; -.
DR UCSC; uc007hyc.1; mouse.
DR CTD; 54606; -.
DR MGI; MGI:1277172; Ddx56.
DR VEuPathDB; HostDB:ENSMUSG00000004393; -.
DR eggNOG; KOG0346; Eukaryota.
DR GeneTree; ENSGT00550000074946; -.
DR HOGENOM; CLU_003041_17_1_1; -.
DR InParanoid; Q9D0R4; -.
DR OMA; GYEKDFK; -.
DR OrthoDB; 973872at2759; -.
DR PhylomeDB; Q9D0R4; -.
DR TreeFam; TF300620; -.
DR BioGRID-ORCS; 52513; 24 hits in 75 CRISPR screens.
DR ChiTaRS; Ddx56; mouse.
DR PRO; PR:Q9D0R4; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q9D0R4; protein.
DR Bgee; ENSMUSG00000004393; Expressed in otic placode and 250 other tissues.
DR ExpressionAtlas; Q9D0R4; baseline and differential.
DR Genevisible; Q9D0R4; MM.
DR GO; GO:0005730; C:nucleolus; IDA:MGI.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0010976; P:positive regulation of neuron projection development; ISO:MGI.
DR GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014014; RNA_helicase_DEAD_Q_motif.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS51195; Q_MOTIF; 1.
PE 2: Evidence at transcript level;
KW ATP-binding; Helicase; Hydrolase; Nucleotide-binding; Nucleus;
KW Phosphoprotein; Reference proteome; Ribosome biogenesis; RNA-binding;
KW rRNA processing.
FT CHAIN 1..546
FT /note="Probable ATP-dependent RNA helicase DDX56"
FT /id="PRO_0000055059"
FT DOMAIN 38..218
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 230..424
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 324..344
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 508..546
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 7..35
FT /note="Q motif"
FT MOTIF 166..169
FT /note="DEAD box"
FT BINDING 51..58
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT MOD_RES 126
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9NY93"
FT MOD_RES 141
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9NY93"
SQ SEQUENCE 546 AA; 61212 MW; 3C19F7354C29F9A1 CRC64;
MEDQEALGFE HMGLDPRLLQ AVTDLGWSRP TLIQEKAIPL ALEGKDLLAR ARTGSGKTAA
YAIPMLQSLL HKKATGPVME QAVRGLVLVP TKELARQAQA MIQQLAAYCA RDVRVANVSA
AEDSASQRAV LMEKPDVVVG TPSRVLSHLQ QNTLKLRDSL ELLVVDEADL LFSFGFEDEL
KSLLCHLPRI YQAFLMSATF NEDVQTLKEL VLHNPVTLKL QESQLPGPDQ LQQFQVVCET
EEDKFLLLYA LLKLSLIRGK ALLFVNTLER GYRLRLFLEQ FSIPSCVLNG ELPLRSRCHI
ISQFNQGLYD CVIATDAEIL GPQVKGKRRG RGSKGNKASD PESGVARGID FHHVSAVLNF
DLPPTAEAYV HRAGRTARAN NPGIVLTFVL PAEQPFLGKI EDLLSGEGEA PILLPYQFQM
EEIESFRYRC RDAMRSVTKQ AIREARLKEI KEELLHSEKL KTYFEDNPRD LQLLRHDLPL
HPAVVKPHLG HVPDYLVPAA LRGLVHPRKK RRKVPFSRKA KKVKAQNPLR DFKHRGKKPK
PAAKPS
