DDX5_DICDI
ID DDX5_DICDI Reviewed; 697 AA.
AC Q54CD6;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Probable ATP-dependent RNA helicase ddx5;
DE EC=3.6.4.13;
DE AltName: Full=DEAD box protein 5;
GN Name=ddx5; ORFNames=DDB_G0293036;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Probable ATP-dependent RNA helicase. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC -!- DOMAIN: The Q motif is unique to and characteristic of the DEAD box
CC family of RNA helicases and controls ATP binding and hydrolysis.
CC -!- SIMILARITY: Belongs to the DEAD box helicase family. DDX5/DBP2
CC subfamily. {ECO:0000305}.
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DR EMBL; AAFI02000199; EAL60870.1; -; Genomic_DNA.
DR RefSeq; XP_629283.1; XM_629281.1.
DR AlphaFoldDB; Q54CD6; -.
DR SMR; Q54CD6; -.
DR STRING; 44689.DDB0266367; -.
DR PaxDb; Q54CD6; -.
DR EnsemblProtists; EAL60870; EAL60870; DDB_G0293036.
DR GeneID; 8629006; -.
DR KEGG; ddi:DDB_G0293036; -.
DR dictyBase; DDB_G0293036; ddx5.
DR eggNOG; KOG0331; Eukaryota.
DR HOGENOM; CLU_003041_1_3_1; -.
DR InParanoid; Q54CD6; -.
DR OMA; YLAPMIP; -.
DR PhylomeDB; Q54CD6; -.
DR PRO; PR:Q54CD6; -.
DR Proteomes; UP000002195; Chromosome 6.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0003724; F:RNA helicase activity; IBA:GO_Central.
DR GO; GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IEA:UniProtKB-KW.
DR GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.300; -; 2.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR000629; RNA-helicase_DEAD-box_CS.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00039; DEAD_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cytoplasm; Helicase; Hydrolase; Nonsense-mediated mRNA decay;
KW Nucleotide-binding; Nucleus; Reference proteome; Ribosome biogenesis;
KW RNA-binding; rRNA processing.
FT CHAIN 1..697
FT /note="Probable ATP-dependent RNA helicase ddx5"
FT /id="PRO_0000327410"
FT DOMAIN 237..425
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT DOMAIN 450..603
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00542"
FT REGION 137..190
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 665..697
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 189..234
FT /note="Q motif"
FT /evidence="ECO:0000250"
FT MOTIF 373..376
FT /note="DEAD box"
FT COMPBIAS 152..190
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 665..688
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 226..228
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
FT BINDING 233
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT BINDING 250..257
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00541"
SQ SEQUENCE 697 AA; 80799 MW; 6707349D32FB23A8 CRC64;
MNKYKNKYNT DNKFNNSKYI KNNQVELSKQ ELEDEEILKI NDDEFNEGLN KQALFQQDDY
VYKFKKQQKE EKIGGDEKTQ IDNEKPEDFN ITLKSIDFKS INLPSFKKRF LQSSTIKDID
SFEFINSTTE FIIENKSDDD NYYDDDDDDE GDKNKEKEKE KEKEKEKEKE KEKEKEKEKE
KEKEKEKNKE IENKTYPNNC KIPIANTGES ECLPNTLKLF FKKTQFPTPT LFQQYAWPAI
LTGHDIIGTS LPGSGKTLGY LAPMIPHCLA RVDRGGKNKI TGEKAPKQYT GILVLVLVPT
RELGLQVHSN TLIITQLFGI KTSVIYGGIS KNLQIEQLEK EKPQILISTP GRLIEMIENG
HVDLSSVTML VLDEADKMLS KGLIPQLKQI RGQIRPDSQN ILFSATFPDS LKEVSKDWIK
DPSIRLRIGS SELPKLNHIQ QDAQLIAHHK KPRALIKLLS EPQFKEKKKT IVFFNKIKEL
KRISIMLLKS NIKHDTIFGN IDQELREKLI NKFSSSRSTL LLSTDIIGRG IHIDDIFNII
NYDFPRSLEQ YCHRVGRAGR SDKVVNPKAF SFLTNSDSYL VEPFVQFLKD QHQNISIPFL
KLCNENFGTK FEFTLSKRKQ KKLAQKDDVE KDDEAYLAKY EKLIKKDRKK DSDDECKKFK
SFKRKNIDSD SDNDSDSNSD NGKETKSKDK NKKFKKY
