3BHS_HORSE
ID 3BHS_HORSE Reviewed; 373 AA.
AC O46516; P79437;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase;
DE Short=3-beta-HSD;
DE Includes:
DE RecName: Full=3-beta-hydroxy-Delta(5)-steroid dehydrogenase;
DE EC=1.1.1.145;
DE AltName: Full=3-beta-hydroxy-5-ene steroid dehydrogenase;
DE AltName: Full=Progesterone reductase;
DE Includes:
DE RecName: Full=Steroid Delta-isomerase;
DE EC=5.3.3.1;
DE AltName: Full=Delta-5-3-ketosteroid isomerase;
GN Name=HSD3B;
OS Equus caballus (Horse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Perissodactyla; Equidae; Equus.
OX NCBI_TaxID=9796;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Ovarian follicle membrane;
RX PubMed=11133676; DOI=10.1095/biolreprod64.1.206;
RA Boerboom D., Sirois J.;
RT "Equine P450 cholesterol side-chain cleavage and 3 beta-hydroxysteroid
RT dehydrogenase/delta(5)-delta(4) isomerase: molecular cloning and regulation
RT of their messenger ribonucleic acids in equine follicles during the
RT ovulatory process.";
RL Biol. Reprod. 64:206-215(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Hasegawa T., Ishida M., Harigaya T., Ishida N., Mukoyama H.;
RT "Molecular cloning, nucleotide sequence and tissue distribution of equine
RT testicular 3 beta-hydroxysteroid dehydrogenase/delta5-delta4 isomerase
RT messenger ribonucleic acid.";
RL J. Equine Sci. 9:45-52(1998).
CC -!- FUNCTION: 3-beta-HSD is a bifunctional enzyme, that catalyzes the
CC oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the
CC oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system
CC plays a crucial role in the biosynthesis of all classes of hormonal
CC steroids.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 3beta-hydroxy-Delta(5)-steroid + NAD(+) = a 3-oxo-Delta(5)-
CC steroid + H(+) + NADH; Xref=Rhea:RHEA:24076, ChEBI:CHEBI:1722,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:47907, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945; EC=1.1.1.145;
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid;
CC Xref=Rhea:RHEA:14709, ChEBI:CHEBI:47907, ChEBI:CHEBI:47909;
CC EC=5.3.3.1;
CC -!- PATHWAY: Lipid metabolism; steroid biosynthesis.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass
CC membrane protein. Mitochondrion membrane; Single-pass membrane protein.
CC -!- SIMILARITY: Belongs to the 3-beta-HSD family. {ECO:0000305}.
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DR EMBL; AF031665; AAC04701.1; -; mRNA.
DR EMBL; D89666; BAA32698.1; -; mRNA.
DR RefSeq; NP_001075380.1; NM_001081911.1.
DR RefSeq; NP_001295566.1; NM_001308637.1.
DR AlphaFoldDB; O46516; -.
DR SMR; O46516; -.
DR STRING; 9796.ENSECAP00000028931; -.
DR PaxDb; O46516; -.
DR Ensembl; ENSECAT00000015327; ENSECAP00000012310; ENSECAG00000014483.
DR GeneID; 100034078; -.
DR KEGG; ecb:100034078; -.
DR CTD; 3284; -.
DR GeneTree; ENSGT00940000155444; -.
DR HOGENOM; CLU_007383_6_3_1; -.
DR InParanoid; O46516; -.
DR OrthoDB; 930591at2759; -.
DR UniPathway; UPA00062; -.
DR Proteomes; UP000002281; Chromosome 5.
DR Bgee; ENSECAG00000014483; Expressed in chorionic villus and 12 other tissues.
DR ExpressionAtlas; O46516; baseline.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IBA:GO_Central.
DR GO; GO:0031966; C:mitochondrial membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0003854; F:3-beta-hydroxy-delta5-steroid dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0102294; F:cholesterol dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0016616; F:oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor; IBA:GO_Central.
DR GO; GO:0004769; F:steroid delta-isomerase activity; IEA:UniProtKB-EC.
DR GO; GO:0008207; P:C21-steroid hormone metabolic process; IBA:GO_Central.
DR GO; GO:0021766; P:hippocampus development; IBA:GO_Central.
DR GO; GO:0051412; P:response to corticosterone; IBA:GO_Central.
DR GO; GO:0006694; P:steroid biosynthetic process; IBA:GO_Central.
DR InterPro; IPR002225; 3Beta_OHSteriod_DH/Estase.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR Pfam; PF01073; 3Beta_HSD; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Isomerase; Membrane; Mitochondrion;
KW Multifunctional enzyme; NAD; Oxidoreductase; Reference proteome;
KW Steroidogenesis; Transmembrane; Transmembrane helix.
FT CHAIN 1..373
FT /note="3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-
FT isomerase"
FT /id="PRO_0000087773"
FT TRANSMEM 288..308
FT /note="Helical"
FT /evidence="ECO:0000255"
FT ACT_SITE 155
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT BINDING 159
FT /ligand="NAD(+)"
FT /ligand_id="ChEBI:CHEBI:57540"
FT /evidence="ECO:0000250"
SQ SEQUENCE 373 AA; 42411 MW; 837A04E3C681417C CRC64;
MAGWSCLVTG AGGFLGQRIV RLLVEEKEVQ EIRALDKVFR PELREEFSKL QSKVKLTVLE
GDILDEQFLK RACQGASAVI HTASIIDVTN LFNPQVTMNV NVEGTQLLLE ACSQASVPIF
IYTSSVAVAG PNSYREIIQN GHEEAHLETK WSSPYPYSKK LAEKAVLAAN GLPLKNGGTL
YTCALRPMFI YGEGSPTLYY LMHEGLNNNG ILTHNCKFSR ANPVYVGNIA WAHIMALRAL
RDPKKAPSIQ GQFYYISDDT PPQSYDDLTY TLSKKWGFCL DSRMRLPIFL KYWLAFLLEI
VSFLLSPIYK YRPPFDRHLV TWQNSVFTFS YKKAQRDMGY EPLFSWEEAK KRTTEWIDAL
VEPHQEALKT KTL
