DEAF1_DROME
ID DEAF1_DROME Reviewed; 576 AA.
AC Q24180;
DT 13-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=Deformed epidermal autoregulatory factor 1;
DE Short=Protein DEAF-1;
GN Name=Deaf1; ORFNames=CG8567;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 252-264 AND 495-519.
RC TISSUE=Embryo;
RX PubMed=8617243; DOI=10.1002/j.1460-2075.1996.tb00547.x;
RA Gross C.T., McGinnis W.;
RT "DEAF-1, a novel protein that binds an essential region in a Deformed
RT response element.";
RL EMBO J. 15:1961-1970(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
CC -!- FUNCTION: Transcription factor that binds the homeotic Deformed (Dfd)
CC response element. High affinity binding sites contain at least 1 TTCG
CC motif surrounded by additional TCG sequences. May be involved in the
CC selective action of Dfd on these sites without binding directly to the
CC Dfd protein. Requirement of DEAF1 activity may be a common feature of
CC enhancers targeted by Dfd.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- DEVELOPMENTAL STAGE: Ubiquitous throughout embryogenesis. Highly
CC expressed in stage 3 embryo indicating that it is maternally provided.
CC After stage 15, it is more prominent in the central nervous system.
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DR EMBL; AE014296; AAF49105.1; -; Genomic_DNA.
DR EMBL; U46686; AAC47040.1; -; mRNA.
DR PIR; S69214; S69214.
DR RefSeq; NP_001262069.1; NM_001275140.1.
DR RefSeq; NP_524169.1; NM_079445.3.
DR AlphaFoldDB; Q24180; -.
DR SMR; Q24180; -.
DR BioGRID; 65433; 27.
DR DIP; DIP-18732N; -.
DR IntAct; Q24180; 21.
DR STRING; 7227.FBpp0304463; -.
DR PaxDb; Q24180; -.
DR DNASU; 40164; -.
DR EnsemblMetazoa; FBtr0074882; FBpp0074651; FBgn0013799.
DR EnsemblMetazoa; FBtr0332153; FBpp0304463; FBgn0013799.
DR GeneID; 40164; -.
DR KEGG; dme:Dmel_CG8567; -.
DR CTD; 10522; -.
DR FlyBase; FBgn0013799; Deaf1.
DR VEuPathDB; VectorBase:FBgn0013799; -.
DR eggNOG; KOG4333; Eukaryota.
DR GeneTree; ENSGT00940000159701; -.
DR InParanoid; Q24180; -.
DR OrthoDB; 662933at2759; -.
DR PhylomeDB; Q24180; -.
DR SignaLink; Q24180; -.
DR BioGRID-ORCS; 40164; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 40164; -.
DR PRO; PR:Q24180; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0013799; Expressed in brain and 32 other tissues.
DR ExpressionAtlas; Q24180; baseline and differential.
DR Genevisible; Q24180; DM.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0005700; C:polytene chromosome; IDA:FlyBase.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:FlyBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:FlyBase.
DR GO; GO:0009792; P:embryo development ending in birth or egg hatching; IMP:FlyBase.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:FlyBase.
DR GO; GO:0050776; P:regulation of immune response; IDA:FlyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IDA:FlyBase.
DR Gene3D; 3.10.390.10; -; 1.
DR InterPro; IPR010919; SAND-like_dom_sf.
DR InterPro; IPR000770; SAND_dom.
DR InterPro; IPR024119; TF_DEAF-1.
DR InterPro; IPR002893; Znf_MYND.
DR PANTHER; PTHR10237; PTHR10237; 2.
DR Pfam; PF01342; SAND; 1.
DR Pfam; PF01753; zf-MYND; 1.
DR SMART; SM00258; SAND; 1.
DR SUPFAM; SSF63763; SSF63763; 1.
DR PROSITE; PS50864; SAND; 1.
DR PROSITE; PS01360; ZF_MYND_1; 1.
DR PROSITE; PS50865; ZF_MYND_2; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; DNA-binding; Metal-binding; Nucleus;
KW Reference proteome; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..576
FT /note="Deformed epidermal autoregulatory factor 1"
FT /id="PRO_0000074088"
FT DOMAIN 210..291
FT /note="SAND"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00185"
FT ZN_FING 521..557
FT /note="MYND-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00134"
FT REGION 52..76
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 189..215
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 309..362
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 324..340
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 52..69
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 323..341
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 346..362
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 521
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00134"
FT BINDING 524
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00134"
FT BINDING 532
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00134"
FT BINDING 535
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00134"
FT BINDING 541
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00134"
FT BINDING 545
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00134"
FT BINDING 553
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00134"
FT BINDING 557
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00134"
SQ SEQUENCE 576 AA; 61598 MW; 2D6EB582685D65BB CRC64;
MEQVDSSTEL HLNRKDLAAL AEDVVKEEVI LESSSHHHHH HHHQLDTKVR MVTSSSNDNS
GSGGASGGTS GAGGGNGGGG VVSVPVSLPI GSMITGTTFN VITPDQLPPH FKPMLCVDNN
GYLSGSTVSM GNDLKTIVIQ QQQTQPGGGG GGANNAGTNT TATNTIGLNH DGSGSNNSHD
SLATLEHAAG GASGVGGGGG GTGGGSSGWS ENPSTQHNEV FQIRCKTTCA ELYRSKLGSG
GRGRCVKYKD KWHTPSEFEH VCGRGSSKDW KRSIKYGGKS LQSLIDEGTL TPHATNCSCT
VCCDDEAGES ASGPVRLFTP YKRRKRNQTD LDMESGPKRK RNTHHSNNNN SNTNNNNTSG
SGANNCVDVT AAVAAATASV VDENNMFLSE ENITSKDEPW AALNDSLDTS TELVDQSQMG
NTYERETFVV NINDGSSIAV LDTSQSMKNI EHVYCTMVKA TNDFKRMLND MKQSFERRIE
VLQKERDAAV SAMRVQVHAD IDDPNISGSL HGNEIISAKK CANCNREALA ECSLCRKTPY
CSEFCQRKDW NAHQVECTRN PQTTTQQVML LIDDQS
