DEAL1_ARATH
ID DEAL1_ARATH Reviewed; 163 AA.
AC Q9ZV57; A0A178VR11;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Protein VASCULATURE COMPLEXITY AND CONNECTIVITY {ECO:0000303|PubMed:25149602};
DE AltName: Full=Protein DESIGUAL 1 {ECO:0000303|PubMed:29139551};
DE Flags: Precursor;
GN Name=VCC {ECO:0000303|PubMed:25149602};
GN Synonyms=DEAL1 {ECO:0000303|PubMed:29139551};
GN OrderedLocusNames=At2g32280 {ECO:0000312|Araport:AT2G32280};
GN ORFNames=T32F6.20 {ECO:0000312|EMBL:AAC69953.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Cheuk R.F., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY,
RP AND INTERACTION WITH OPS.
RC STRAIN=cv. Columbia;
RX PubMed=25149602; DOI=10.1104/pp.114.246314;
RA Roschzttardtz H., Paez-Valencia J., Dittakavi T., Jali S., Reyes F.C.,
RA Baisa G., Anne P., Gissot L., Palauqui J.-C., Masson P.H., Bednarek S.Y.,
RA Otegui M.S.;
RT "The VASCULATURE COMPLEXITY AND CONNECTIVITY gene encodes a plant-specific
RT protein required for embryo provasculature development.";
RL Plant Physiol. 166:889-902(2014).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP DEVELOPMENTAL STAGE, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=29139551; DOI=10.1111/nph.14898;
RA Wilson-Sanchez D., Martinez-Lopez S., Navarro-Cartagena S., Jover-Gil S.,
RA Micol J.L.;
RT "Members of the DEAL subfamily of the DUF1218 gene family are required for
RT bilateral symmetry but not for dorsoventrality in Arabidopsis leaves.";
RL New Phytol. 217:1307-1321(2018).
RN [7]
RP REVIEW ON EMBRYO-DEFECTIVE MUTANTS.
RX PubMed=31334862; DOI=10.1111/nph.16071;
RA Meinke D.W.;
RT "Genome-wide identification of EMBRYO-DEFECTIVE (EMB) genes required for
RT growth and development in Arabidopsis.";
RL New Phytol. 226:306-325(2020).
CC -!- FUNCTION: Required, together with OPS, for embryo provasculature
CC development and cotyledon vascular complexity and connectivity
CC (PubMed:25149602). Necessary, partially redundantly with DEAL2 and
CC DEAL3, to ensure bilateral symmetry development and early leaf margin
CC patterning, probably via the regulation of auxin and CUC2 distribution
CC (PubMed:29139551). Regulates cell proliferation but not cell expansion
CC (PubMed:29139551). {ECO:0000269|PubMed:25149602,
CC ECO:0000269|PubMed:29139551}.
CC -!- SUBUNIT: Interacts with OPS. {ECO:0000269|PubMed:25149602}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:29139551}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in vascular cells, mostly in hypocotyls,
CC and, to a lower extent, in seedlings, roots, flowers, siliques,
CC developing leaves and inflorescences, but barely in mature leaves and
CC seeds (PubMed:25149602, PubMed:29139551). High levels in leaf primordia
CC (PubMed:29139551). {ECO:0000269|PubMed:25149602,
CC ECO:0000269|PubMed:29139551}.
CC -!- DEVELOPMENTAL STAGE: Mainly present in dividing tissues and fades out
CC during cells differentiation, to reappear later in the vasculature
CC (PubMed:29139551). Expressed in developing embryos and procambial,
CC cambial, and vascular cells of cotyledons, leaves, roots, hypocotyls,
CC and anthers (PubMed:25149602). During leaves development, first
CC observed in leaf primordia, and becomes localized to the margins and
CC the base of the lamina during the transition from cell proliferation to
CC expansion and differentiation (PubMed:29139551). Later confined to
CC petiole primordia (PubMed:29139551). Also detected in flower primordia
CC (PubMed:29139551). {ECO:0000269|PubMed:25149602,
CC ECO:0000269|PubMed:29139551}.
CC -!- DISRUPTION PHENOTYPE: Defects in vein connectivity early in mutant
CC embryo development leading to reduced complexity of cotyledon vein
CC networks and disconnected veins, including in embryos provasculatures
CC (PubMed:25149602). The mutant deal1-1 exhibits a bilateral symmetry
CC breaking but no obvious defects in dorsoventrality; early leaf
CC primordia fail to acquire bilateral symmetry and instead form ectopic
CC lobes and sinuses (PubMed:29139551). Developmental defects of pistils,
CC some being bent or coiled or twisted, and some showing unfused carpels
CC with exposed ovules (PubMed:29139551). In leaves, marginal cells
CC expressing properly polarized PIN1 are badly recruited, thus leading to
CC misplaced auxin maxima, as well as defects in cell proliferation but
CC not in cell expansion (PubMed:29139551). Altered spatial pattern of
CC CUC2 (PubMed:29139551). The double mutant vcc ops exhibits a complete
CC loss of high-complexity vascular networks (PubMed:25149602). The vcc-3
CC deal2-1 and vcc-3 deal3-1 double mutants show leaf asymmetry
CC (PubMed:29139551). The vcc-3 deal2-1 deal3-1 triple mutant shows a
CC strong leaf asymmetry (PubMed:29139551). {ECO:0000269|PubMed:25149602,
CC ECO:0000269|PubMed:29139551}.
CC -!- SIMILARITY: Belongs to the DESIGUAL family. {ECO:0000305}.
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DR EMBL; AC005700; AAC69953.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08662.1; -; Genomic_DNA.
DR EMBL; CP002685; ANM61199.1; -; Genomic_DNA.
DR EMBL; BT011667; AAS47673.1; -; mRNA.
DR EMBL; AK175644; BAD43407.1; -; mRNA.
DR PIR; B84731; B84731.
DR RefSeq; NP_001323429.1; NM_001336384.1.
DR RefSeq; NP_180787.1; NM_128787.4.
DR AlphaFoldDB; Q9ZV57; -.
DR STRING; 3702.AT2G32280.1; -.
DR TCDB; 8.A.175.1.1; the vasculature complexity and connectivity (vcc) family.
DR PaxDb; Q9ZV57; -.
DR PRIDE; Q9ZV57; -.
DR ProteomicsDB; 180908; -.
DR EnsemblPlants; AT2G32280.1; AT2G32280.1; AT2G32280.
DR EnsemblPlants; AT2G32280.2; AT2G32280.2; AT2G32280.
DR GeneID; 817788; -.
DR Gramene; AT2G32280.1; AT2G32280.1; AT2G32280.
DR Gramene; AT2G32280.2; AT2G32280.2; AT2G32280.
DR KEGG; ath:AT2G32280; -.
DR Araport; AT2G32280; -.
DR TAIR; locus:2062561; AT2G32280.
DR eggNOG; ENOG502RXRV; Eukaryota.
DR HOGENOM; CLU_103513_0_0_1; -.
DR InParanoid; Q9ZV57; -.
DR OMA; FSHHHFF; -.
DR OrthoDB; 1382746at2759; -.
DR PhylomeDB; Q9ZV57; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9ZV57; baseline and differential.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0010252; P:auxin homeostasis; IMP:UniProtKB.
DR GO; GO:0048825; P:cotyledon development; IMP:TAIR.
DR GO; GO:0010588; P:cotyledon vascular tissue pattern formation; IMP:TAIR.
DR GO; GO:0009855; P:determination of bilateral symmetry; IMP:TAIR.
DR GO; GO:0048366; P:leaf development; IMP:TAIR.
DR GO; GO:0010305; P:leaf vascular tissue pattern formation; IMP:TAIR.
DR GO; GO:0010087; P:phloem or xylem histogenesis; IMP:TAIR.
DR GO; GO:0051302; P:regulation of cell division; IMP:UniProtKB.
DR InterPro; IPR009606; DEAL/Modifying_wall_lignin1/2.
DR Pfam; PF06749; DUF1218; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Endoplasmic reticulum; Membrane; Reference proteome;
KW Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..27
FT /evidence="ECO:0000255"
FT CHAIN 28..163
FT /note="Protein VASCULATURE COMPLEXITY AND CONNECTIVITY"
FT /id="PRO_0000454967"
FT TRANSMEM 54..74
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 95..115
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..153
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 163 AA; 17481 MW; C19806D5BC925B22 CRC64;
MTKIGGILVC LVIVGLDVAA AILGIQAEVA QNQVKHMRLW LFECREPSQD AFRLGLGAAA
ILVMAHVLLN LVGGCLCICS QDEFQRSSST RQISMACLVL TWIVFAVGFG SIVIGTMSNS
KSRSSCGFTH HHFLSIGGIL CFLHALFCVA YYVSATAAKD EAK
