DEC12_DROME
ID DEC12_DROME Reviewed; 950 AA.
AC P18170; P92153; P92154; Q24299; Q26309; Q26310; Q26311; Q8IRP0;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 16-MAY-2003, sequence version 2.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Defective chorion-1 protein, FC106 isoform;
DE Contains:
DE RecName: Full=Defective chorion-1 protein, FC106 isoform;
DE Contains:
DE RecName: Full=FC106 S80;
DE Contains:
DE RecName: Full=FC106 S60;
DE Flags: Precursor;
GN Name=dec-1; ORFNames=CG2175;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 279-299, AND ALTERNATIVE
RP SPLICING.
RX PubMed=1699826; DOI=10.1016/0012-1606(90)90146-a;
RA Waring G.L., Hawley R.J., Schoenfeld T.;
RT "Multiple proteins are produced from the dec-1 eggshell gene in Drosophila
RT by alternative RNA splicing and proteolytic cleavage events.";
RL Dev. Biol. 142:1-12(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE OF 1-369 AND 425-596.
RC STRAIN=Samarkand;
RX PubMed=1774787; DOI=10.1007/bf02102863;
RA Andersson S., Lambertsson A.;
RT "Evolution of the dec-1 eggshell locus in Drosophila. I. Restriction site
RT mapping and limited sequence comparison in the melanogaster species
RT subgroup.";
RL J. Mol. Evol. 33:321-331(1991).
RN [5]
RP NUCLEOTIDE SEQUENCE OF 493-698 (ALLELES FC1; FC2; FC3 AND FC4).
RC STRAIN=Dilizhan, Ghanghry, Israel, Samarkand, and Shahrinau;
RX PubMed=8350348; DOI=10.1007/bf00556358;
RA Andersson S., Lambertsson A.;
RT "Evolution of the dec-1 eggshell locus in Drosophila. II. Intraspecific DNA
RT sequence analysis reveals length mutations in a repetitive region in D.
RT melanogaster.";
RL J. Mol. Evol. 36:536-544(1993).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=3378704; DOI=10.1101/gad.2.3.341;
RA Hawley R.J., Waring G.L.;
RT "Cloning and analysis of the dec-1 female-sterile locus, a gene required
RT for proper assembly of the Drosophila eggshell.";
RL Genes Dev. 2:341-349(1988).
CC -!- FUNCTION: Required for proper assembly of the eggshell.
CC {ECO:0000269|PubMed:3378704}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:3378704}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=FC106;
CC IsoId=P18170-1; Sequence=Displayed;
CC Name=FC125;
CC IsoId=P18169-1; Sequence=External;
CC Name=FC177;
CC IsoId=P18171-1; Sequence=External;
CC -!- DEVELOPMENTAL STAGE: Synthesized in early stage 10 egg chambers.
CC Cleavage generates S80, a 80 kDa species, during late stage 10 which is
CC incorporated into the eggshell. During the latter stages of chorion
CC formation, S80 is processed to a 60 kDa component, S60.
CC {ECO:0000269|PubMed:3378704}.
CC -!- PTM: Proteolytic cleavage of isoform FC106 generates 2 further
CC products, S80 and S60.
CC -!- MISCELLANEOUS: Allele FC1 is found only in strain Samarkand, allele FC3
CC only in strain Shahrinau and FC4 only in strain Dilizhan. Allele FC2 Is
CC found in both strains Israel and Ghanghry.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA28447.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; M35888; AAA28447.1; ALT_FRAME; mRNA.
DR EMBL; AE014298; AAN09216.1; -; Genomic_DNA.
DR EMBL; X76230; CAA53815.1; -; Genomic_DNA.
DR EMBL; X76231; CAA53816.1; -; Genomic_DNA.
DR EMBL; S64529; AAB27801.2; -; Genomic_DNA.
DR EMBL; S64530; AAB27802.2; -; Genomic_DNA.
DR EMBL; S64531; AAB27803.2; -; Genomic_DNA.
DR EMBL; S64532; AAB27804.2; -; Genomic_DNA.
DR EMBL; S64534; AAB27805.2; -; Genomic_DNA.
DR PIR; B44766; B44766.
DR RefSeq; NP_727203.1; NM_167133.2. [P18170-1]
DR AlphaFoldDB; P18170; -.
DR BioGRID; 58165; 3.
DR DNASU; 31691; -.
DR EnsemblMetazoa; FBtr0071147; FBpp0071099; FBgn0000427. [P18170-1]
DR GeneID; 31691; -.
DR CTD; 31691; -.
DR FlyBase; FBgn0000427; dec-1.
DR VEuPathDB; VectorBase:FBgn0000427; -.
DR HOGENOM; CLU_008795_0_0_1; -.
DR BioGRID-ORCS; 31691; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 31691; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0000427; Expressed in adult abdomen and 11 other tissues.
DR ExpressionAtlas; P18170; baseline and differential.
DR Genevisible; P18170; DM.
DR GO; GO:0042600; C:egg chorion; IDA:UniProtKB.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000987; F:cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0005213; F:structural constituent of egg chorion; IMP:UniProtKB.
DR GO; GO:0007304; P:chorion-containing eggshell formation; IMP:FlyBase.
DR GO; GO:0007306; P:eggshell chorion assembly; IMP:UniProtKB.
DR GO; GO:0043619; P:regulation of transcription from RNA polymerase II promoter in response to oxidative stress; IBA:GO_Central.
DR GO; GO:0042594; P:response to starvation; IBA:GO_Central.
DR InterPro; IPR006720; DEC-1_C.
DR InterPro; IPR006719; DEC-1_N.
DR InterPro; IPR006718; DEC-1_REPEAT.
DR Pfam; PF04624; Dec-1; 8.
DR Pfam; PF04626; DEC-1_C; 1.
DR Pfam; PF04625; DEC-1_N; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Direct protein sequencing; Reference proteome;
KW Repeat; Secreted; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..950
FT /note="Defective chorion-1 protein, FC106 isoform"
FT /id="PRO_0000021098"
FT CHAIN 279..950
FT /note="FC106 S80"
FT /id="PRO_0000021099"
FT CHAIN 279..?
FT /note="FC106 S60"
FT /id="PRO_0000021100"
FT REPEAT 493..518
FT /note="1"
FT REPEAT 519..544
FT /note="2"
FT REPEAT 545..570
FT /note="3"
FT REPEAT 571..596
FT /note="4"
FT REPEAT 597..622
FT /note="5"
FT REPEAT 623..652
FT /note="6; approximate"
FT REPEAT 653..680
FT /note="7; approximate"
FT REPEAT 681..696
FT /note="8; approximate"
FT REPEAT 697..720
FT /note="9; approximate"
FT REPEAT 721..733
FT /note="10; approximate"
FT REPEAT 734..758
FT /note="11; approximate"
FT REPEAT 759..788
FT /note="12; approximate"
FT REGION 23..60
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 184..212
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 268..294
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 493..788
FT /note="12 X 26 AA approximate tandem repeats, Glu, Met-
FT rich"
FT REGION 843..875
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 23..46
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 184..211
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 851..865
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 545..622
FT /note="Missing (in allele DEC-1-FC4)"
FT VARIANT 545..570
FT /note="Missing (in allele DEC-1-FC2-Israel)"
FT VARIANT 571..622
FT /note="Missing (in allele DEC-1-FC3)"
FT VARIANT 597..622
FT /note="Missing (in allele DEC-1-FC2-Ghanghry)"
FT VARIANT 627
FT /note="M -> V (in allele DEC-1-FC3)"
FT VARIANT 644
FT /note="Q -> H (in allele DEC-1-FC4)"
FT VARIANT 690
FT /note="Q -> H (in allele DEC-1-FC3)"
FT CONFLICT 17
FT /note="A -> V (in Ref. 1; AAA28447 and 4; CAA53815)"
FT /evidence="ECO:0000305"
FT CONFLICT 219
FT /note="Q -> E (in Ref. 1; AAA28447)"
FT /evidence="ECO:0000305"
FT CONFLICT 347
FT /note="D -> H (in Ref. 1; AAA28447)"
FT /evidence="ECO:0000305"
FT CONFLICT 382
FT /note="A -> T (in Ref. 1; AAA28447)"
FT /evidence="ECO:0000305"
FT CONFLICT 847..877
FT /note="PENEGTARHKVDALGVGGNKRKKSKSKSAPP -> AGERRHRQAQSRCPGSW
FT RQQAQEVQVQVGAA (in Ref. 1; AAA28447)"
FT /evidence="ECO:0000305"
FT CONFLICT 888..901
FT /note="QRPVVQSYGTSYGG -> SVRWFRVTEQATAE (in Ref. 1)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 950 AA; 108371 MW; 25340DA159E9F508 CRC64;
MRLFSLLPLL ALLVVQAAGQ SEVTSDDPAT DAGSTTNSTT DTKPRIPSQD EILGQMPSIN
PIRTGNPQMD AFYMMFPALG SLLKWGSLFP AYSILGAIPD NLQPTAAASK VVLVLADDAT
AKTRVARQNP PPNPLGQLMN WPALPQDFQL PSMDLGPQVG SFLAQLPAMP TVPGLLGAAA
PVPAPAPAPA AAPPPAPAPA ADPPAAPVPD APQPAILGQA ALQNAFTFFN PANFDASSLL
GQSVPTFAPP NLDFVAQMQR QFFPGMTPAQ PAAAGTDAQA SDISEVRVRP EDPYSQEAQM
KIKSALEMEQ ERQQQAQVKD QEQVPLLWFR MPTTQNQDAT EEKTLEDLRV EAKLRAFERQ
VIAELRMLQK IELMAKQMRS SAAAQNGDSP YRISYPLSRT PIHKITRADI EQALRDDYVR
RLVNKEAQRR ARNSGINTQK ANALKRQAKS QDQTLSKEDI VQIMAYAYRM ANEQMESEKG
KQDKVYAAYR TEQNPMMMQQ RQWSEEQAKI QQNQQQIQQN PMMMQQRQWS EEQAKIQQNQ
QQIQQNPMMM QQRQWSEEQA KIQQNQQQIQ QNPMMMQQRQ WSEEQAKIQQ NQQQIQQNPM
MVQQRQWSEE QAKIQQNQQQ IQQNPMMMQQ RQWSEEQAKI QHDQQMAQQM AQQGLMMTEQ
RQRQWSEDQA KIQQAQQMAQ QTPMMMPQMQ QRQWTEDPQM VQQMQQRQWA EDQTRMQMAQ
QNPMMQQQRQ MAENPQMMQQ RQWSEEQTKI EQAQQMAQQN QMMMQQMQQR QWSEDQAQIQ
QQQRQMMQQT PMMMKERQWA EENPQSVQQQ GPMMMQQQMP SMMQREVEDE DNKAEDDLVG
EAGPQMPENE GTARHKVDAL GVGGNKRKKS KSKSAPPTVI NYYYAAPQRP VVQSYGTSYG
GGGYGSNAYG VPRPVNSYQS QGYRAAVGND EVDEMLRQHQ TMARATHFRQ
