ID   DEDA_BORBU              Reviewed;         204 AA.
AC   O51266;
DT   14-MAY-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=Inner membrane protein BB_0250;
GN   OrderedLocusNames=BB_0250;
OS   Borreliella burgdorferi (strain ATCC 35210 / DSM 4680 / CIP 102532 / B31)
OS   (Borrelia burgdorferi).
OC   Bacteria; Spirochaetes; Spirochaetales; Borreliaceae; Borreliella.
OX   NCBI_TaxID=224326;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35210 / DSM 4680 / CIP 102532 / B31;
RX   PubMed=9403685; DOI=10.1038/37551;
RA   Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A.,
RA   Lathigra R., White O., Ketchum K.A., Dodson R.J., Hickey E.K., Gwinn M.L.,
RA   Dougherty B.A., Tomb J.-F., Fleischmann R.D., Richardson D.L.,
RA   Peterson J.D., Kerlavage A.R., Quackenbush J., Salzberg S.L., Hanson M.,
RA   van Vugt R., Palmer N., Adams M.D., Gocayne J.D., Weidman J.F.,
RA   Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C.,
RA   Garland S.A., Fujii C., Cotton M.D., Horst K., Roberts K.M., Hatch B.,
RA   Smith H.O., Venter J.C.;
RT   "Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi.";
RL   Nature 390:580-586(1997).
RN   [2]
RP   FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 35210 / DSM 4680 / CIP 102532 / B31;
RX   PubMed=20870761; DOI=10.1128/jb.00571-10;
RA   Liang F.T., Xu Q., Sikdar R., Xiao Y., Cox J.S., Doerrler W.T.;
RT   "BB0250 of Borrelia burgdorferi is a conserved and essential inner membrane
RT   protein required for cell division.";
RL   J. Bacteriol. 192:6105-6115(2010).
RN   [3]
RP   DISRUPTION PHENOTYPE, AND MUTAGENESIS OF GLU-39 AND ASP-40.
RX   PubMed=24277026; DOI=10.1128/aac.02238-13;
RA   Kumar S., Doerrler W.T.;
RT   "Members of the conserved DedA family are likely membrane transporters and
RT   are required for drug resistance in Escherichia coli.";
RL   Antimicrob. Agents Chemother. 58:923-930(2014).
CC   -!- FUNCTION: Required for proper cell division and envelope integrity.
CC       {ECO:0000269|PubMed:20870761}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000269|PubMed:20870761}; Multi-pass membrane protein
CC       {ECO:0000269|PubMed:20870761}.
CC   -!- DISRUPTION PHENOTYPE: Depletion results in abnormal membrane
CC       phospholipid composition, defects in cell division and cell death.
CC       {ECO:0000269|PubMed:20870761, ECO:0000269|PubMed:24277026}.
CC   -!- MISCELLANEOUS: Can restore normal growth and cell division to a E.coli
CC       double mutant lacking both yghB and yqjA.
CC       {ECO:0000305|PubMed:20870761}.
CC   -!- SIMILARITY: Belongs to the DedA family. {ECO:0000305}.
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DR   EMBL; AE000783; AAB91496.1; -; Genomic_DNA.
DR   PIR; B70131; B70131.
DR   RefSeq; NP_212384.1; NC_001318.1.
DR   RefSeq; WP_002657683.1; NC_001318.1.
DR   AlphaFoldDB; O51266; -.
DR   STRING; 224326.BB_0250; -.
DR   DNASU; 1195087; -.
DR   EnsemblBacteria; AAB91496; AAB91496; BB_0250.
DR   GeneID; 56567680; -.
DR   KEGG; bbu:BB_0250; -.
DR   PATRIC; fig|224326.49.peg.649; -.
DR   HOGENOM; CLU_044208_4_1_12; -.
DR   OMA; MHIILEF; -.
DR   Proteomes; UP000001807; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR032818; DedA.
DR   InterPro; IPR032816; SNARE_assoc.
DR   PANTHER; PTHR30353; PTHR30353; 1.
DR   Pfam; PF09335; SNARE_assoc; 1.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..204
FT                   /note="Inner membrane protein BB_0250"
FT                   /id="PRO_0000429152"
FT   TRANSMEM        17..37
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        58..78
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        101..121
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        139..159
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        172..192
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   MUTAGEN         39
FT                   /note="E->A: Unable to restore normal growth and cell
FT                   division to the E.coli yghB-yqjA double mutant."
FT                   /evidence="ECO:0000269|PubMed:24277026"
FT   MUTAGEN         40
FT                   /note="D->A: Unable to restore normal growth and cell
FT                   division to the E.coli yghB-yqjA double mutant."
FT                   /evidence="ECO:0000269|PubMed:24277026"
SQ   SEQUENCE   204 AA;  23142 MW;  5D742E27FA2A299F CRC64;
     MTKMYINTII EYIDSNIAYS PIVFFSLLIL AGLNVPISED AIVLMGGILS SRKNEYTILI
     FLGIFWGAYL GDIISFYIGK LMGNKLFKNK KDNNLLDKIN YYYGQYGVLT LFIGRFIPFG
     VRNAIFMSAG MGNMKSNLFI VSDFFATLLS IVVYFTLSFK LGQSFEIIFS KIKIIIFAIF
     IAVIATTIII YVIKKNKKVD KNLK