DEF01_ARATH
ID DEF01_ARATH Reviewed; 77 AA.
AC Q9ZUL7; Q4VP02;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Defensin-like protein 1;
DE AltName: Full=Low-molecular-weight cysteine-rich protein 68;
DE Short=Protein LCR68;
DE AltName: Full=Plant defensin 2.3;
DE Flags: Precursor;
GN Name=PDF2.3; Synonyms=LCR68; OrderedLocusNames=At2g02130; ORFNames=F5O4.10;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 1-30, AND GENE FAMILY.
RX PubMed=15955924; DOI=10.1104/pp.105.060079;
RA Silverstein K.A.T., Graham M.A., Paape T.D., VandenBosch K.A.;
RT "Genome organization of more than 300 defensin-like genes in Arabidopsis.";
RL Plant Physiol. 138:600-610(2005).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=9001391; DOI=10.1016/s0014-5793(96)01378-6;
RA Epple P., Apel K., Bohlmann H.;
RT "ESTs reveal a multigene family for plant defensins in Arabidopsis
RT thaliana.";
RL FEBS Lett. 400:168-172(1997).
RN [6]
RP IDENTIFICATION.
RX PubMed=11437247; DOI=10.1023/a:1010664704926;
RA Vanoosthuyse V., Miege C., Dumas C., Cock J.M.;
RT "Two large Arabidopsis thaliana gene families are homologous to the
RT Brassica gene superfamily that encodes pollen coat proteins and the male
RT component of the self-incompatibility response.";
RL Plant Mol. Biol. 46:17-34(2001).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12447532; DOI=10.1007/s00425-002-0902-6;
RA Thomma B.P.H.J., Cammue B.P., Thevissen K.;
RT "Plant defensins.";
RL Planta 216:193-202(2002).
CC -!- FUNCTION: Confers broad-spectrum resistance to pathogens.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in the whole plant except roots.
CC {ECO:0000269|PubMed:9001391}.
CC -!- SIMILARITY: Belongs to the DEFL family. {ECO:0000305}.
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DR EMBL; AC005936; AAC97221.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC05549.1; -; Genomic_DNA.
DR EMBL; AY050979; AAK93656.1; -; mRNA.
DR EMBL; AY079405; AAL85136.1; -; mRNA.
DR EMBL; AY803260; AAX39301.1; -; mRNA.
DR PIR; C84433; C84433.
DR RefSeq; NP_178321.1; NM_126273.4.
DR AlphaFoldDB; Q9ZUL7; -.
DR SMR; Q9ZUL7; -.
DR BioGRID; 147; 1.
DR IntAct; Q9ZUL7; 1.
DR STRING; 3702.AT2G02130.1; -.
DR PaxDb; Q9ZUL7; -.
DR PRIDE; Q9ZUL7; -.
DR ProteomicsDB; 224589; -.
DR EnsemblPlants; AT2G02130.1; AT2G02130.1; AT2G02130.
DR GeneID; 814744; -.
DR Gramene; AT2G02130.1; AT2G02130.1; AT2G02130.
DR KEGG; ath:AT2G02130; -.
DR Araport; AT2G02130; -.
DR TAIR; locus:2051638; AT2G02130.
DR eggNOG; ENOG502S7HM; Eukaryota.
DR HOGENOM; CLU_161668_1_2_1; -.
DR InParanoid; Q9ZUL7; -.
DR OMA; CKTEKFT; -.
DR OrthoDB; 1625543at2759; -.
DR PhylomeDB; Q9ZUL7; -.
DR PRO; PR:Q9ZUL7; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9ZUL7; baseline and differential.
DR Genevisible; Q9ZUL7; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0006952; P:defense response; ISS:TAIR.
DR GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR CDD; cd00107; Knot1; 1.
DR Gene3D; 3.30.30.10; -; 1.
DR InterPro; IPR008176; Defensin_plant.
DR InterPro; IPR003614; Scorpion_toxin-like.
DR InterPro; IPR036574; Scorpion_toxin-like_sf.
DR PRINTS; PR00288; PUROTHIONIN.
DR SMART; SM00505; Knot1; 1.
DR SUPFAM; SSF57095; SSF57095; 1.
DR PROSITE; PS00940; GAMMA_THIONIN; 1.
PE 2: Evidence at transcript level;
KW Antimicrobial; Disulfide bond; Fungicide; Plant defense;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..30
FT /evidence="ECO:0000255"
FT CHAIN 31..77
FT /note="Defensin-like protein 1"
FT /id="PRO_0000007023"
FT DISULFID 33..77
FT /evidence="ECO:0000250"
FT DISULFID 44..64
FT /evidence="ECO:0000250"
FT DISULFID 50..71
FT /evidence="ECO:0000250"
FT DISULFID 54..73
FT /evidence="ECO:0000250"
SQ SEQUENCE 77 AA; 8544 MW; 0C80DC561971262E CRC64;
MKLSVRFISA ALLLFMVFIA TGMGPVTVEA RTCESKSHRF KGPCVSTHNC ANVCHNEGFG
GGKCRGFRRR CYCTRHC
