DEF02_ARATH
ID DEF02_ARATH Reviewed; 77 AA.
AC Q39182; Q42011; Q4VP00; Q4VP03;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Defensin-like protein 2;
DE AltName: Full=Low-molecular-weight cysteine-rich protein 69;
DE Short=Protein LCR69;
DE AltName: Full=Plant defensin 2.2;
DE Flags: Precursor;
GN Name=PDF2.2; Synonyms=LCR69; OrderedLocusNames=At2g02100; ORFNames=F5O4.13;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND GENE FAMILY.
RX PubMed=15955924; DOI=10.1104/pp.105.060079;
RA Silverstein K.A.T., Graham M.A., Paape T.D., VandenBosch K.A.;
RT "Genome organization of more than 300 defensin-like genes in Arabidopsis.";
RL Plant Physiol. 138:600-610(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. C24; TISSUE=Flower bud;
RA Yu D.-Y., Quigley F., Mache R.;
RT "Isolation and expression of a cDNA encoding protease inhibitor
RT precursor.";
RL Submitted (NOV-1992) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-75.
RC STRAIN=cv. C24; TISSUE=Flower bud;
RX PubMed=8281187; DOI=10.1046/j.1365-313x.1993.04061051.x;
RA Hoefte H., Desprez T., Amselem J., Chiapello H., Rouze P., Caboche M.,
RA Moisan A., Jourjon M.-F., Charpenteau J.-L., Berthomieu P., Guerrier D.,
RA Giraudat J., Quigley F., Thomas F., Yu D.-Y., Mache R., Raynal M.,
RA Cooke R., Grellet F., Delseny M., Parmentier Y., de Marcillac G., Gigot C.,
RA Fleck J., Philipps G., Axelos M., Bardet C., Tremousaygue D., Lescure B.;
RT "An inventory of 1152 expressed sequence tags obtained by partial
RT sequencing of cDNAs from Arabidopsis thaliana.";
RL Plant J. 4:1051-1061(1993).
RN [7]
RP TISSUE SPECIFICITY.
RA Thomma B.P.H.J., Broekaert W.F.;
RT "Tissue-specific expression of plant defensin genes PDF2.1 and PDF2.2 in
RT Arabidopsis thaliana.";
RL Plant Physiol. Biochem. 36:533-537(1998).
RN [8]
RP IDENTIFICATION, AND TISSUE SPECIFICITY.
RX PubMed=11437247; DOI=10.1023/a:1010664704926;
RA Vanoosthuyse V., Miege C., Dumas C., Cock J.M.;
RT "Two large Arabidopsis thaliana gene families are homologous to the
RT Brassica gene superfamily that encodes pollen coat proteins and the male
RT component of the self-incompatibility response.";
RL Plant Mol. Biol. 46:17-34(2001).
RN [9]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12447532; DOI=10.1007/s00425-002-0902-6;
RA Thomma B.P.H.J., Cammue B.P., Thevissen K.;
RT "Plant defensins.";
RL Planta 216:193-202(2002).
CC -!- FUNCTION: Confers broad-spectrum resistance to pathogens.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Broadly expressed. {ECO:0000269|PubMed:11437247,
CC ECO:0000269|Ref.7}.
CC -!- SIMILARITY: Belongs to the DEFL family. {ECO:0000305}.
CC -!- CAUTION: Was initially thought (Ref.2) to be a protease inhibitor.
CC {ECO:0000305}.
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DR EMBL; AY803259; AAX39300.1; -; mRNA.
DR EMBL; AY803262; AAX39303.1; -; mRNA.
DR EMBL; X69139; CAA48892.1; -; mRNA.
DR EMBL; AC005936; AAC97223.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC05546.1; -; Genomic_DNA.
DR EMBL; AF370355; AAK44170.1; -; mRNA.
DR EMBL; Z18455; CAA79189.1; -; mRNA.
DR PIR; S30578; S30578.
DR RefSeq; NP_178319.1; NM_126271.3.
DR AlphaFoldDB; Q39182; -.
DR SMR; Q39182; -.
DR BioGRID; 144; 1.
DR IntAct; Q39182; 1.
DR STRING; 3702.AT2G02100.1; -.
DR PaxDb; Q39182; -.
DR PRIDE; Q39182; -.
DR ProteomicsDB; 224044; -.
DR EnsemblPlants; AT2G02100.1; AT2G02100.1; AT2G02100.
DR GeneID; 814741; -.
DR Gramene; AT2G02100.1; AT2G02100.1; AT2G02100.
DR KEGG; ath:AT2G02100; -.
DR Araport; AT2G02100; -.
DR TAIR; locus:2051668; AT2G02100.
DR eggNOG; ENOG502S7HM; Eukaryota.
DR HOGENOM; CLU_161668_1_2_1; -.
DR InParanoid; Q39182; -.
DR OMA; WCARTER; -.
DR OrthoDB; 1625543at2759; -.
DR PhylomeDB; Q39182; -.
DR PRO; PR:Q39182; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q39182; baseline and differential.
DR Genevisible; Q39182; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0009505; C:plant-type cell wall; HDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0006952; P:defense response; ISS:TAIR.
DR GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR CDD; cd00107; Knot1; 1.
DR Gene3D; 3.30.30.10; -; 1.
DR InterPro; IPR008176; Defensin_plant.
DR InterPro; IPR003614; Scorpion_toxin-like.
DR InterPro; IPR036574; Scorpion_toxin-like_sf.
DR PRINTS; PR00288; PUROTHIONIN.
DR SMART; SM00505; Knot1; 1.
DR SUPFAM; SSF57095; SSF57095; 1.
DR PROSITE; PS00940; GAMMA_THIONIN; 1.
PE 2: Evidence at transcript level;
KW Antimicrobial; Disulfide bond; Fungicide; Plant defense;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..30
FT /evidence="ECO:0000255"
FT CHAIN 31..77
FT /note="Defensin-like protein 2"
FT /id="PRO_0000007024"
FT DISULFID 33..77
FT /evidence="ECO:0000250"
FT DISULFID 44..64
FT /evidence="ECO:0000250"
FT DISULFID 50..71
FT /evidence="ECO:0000250"
FT DISULFID 54..73
FT /evidence="ECO:0000250"
SQ SEQUENCE 77 AA; 8524 MW; EC04E660C3610965 CRC64;
MKLSMRLISA VLIMFMIFVA TGMGPVTVEA RTCESQSHRF KGTCVSASNC ANVCHNEGFV
GGNCRGFRRR CFCTRHC
