DEF04_ARATH
ID DEF04_ARATH Reviewed; 77 AA.
AC Q41914; Q541X5; Q9ZUL6;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Defensin-like protein 4;
DE AltName: Full=Low-molecular-weight cysteine-rich protein 70;
DE Short=Protein LCR70;
DE AltName: Full=Plant defensin 2.1;
DE Flags: Precursor;
GN Name=PDF2.1; Synonyms=LCR70; OrderedLocusNames=At2g02120; ORFNames=F5O4.11;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia; TISSUE=Green siliques;
RX PubMed=8281187; DOI=10.1046/j.1365-313x.1993.04061051.x;
RA Hoefte H., Desprez T., Amselem J., Chiapello H., Rouze P., Caboche M.,
RA Moisan A., Jourjon M.-F., Charpenteau J.-L., Berthomieu P., Guerrier D.,
RA Giraudat J., Quigley F., Thomas F., Yu D.-Y., Mache R., Raynal M.,
RA Cooke R., Grellet F., Delseny M., Parmentier Y., de Marcillac G., Gigot C.,
RA Fleck J., Philipps G., Axelos M., Bardet C., Tremousaygue D., Lescure B.;
RT "An inventory of 1152 expressed sequence tags obtained by partial
RT sequencing of cDNAs from Arabidopsis thaliana.";
RL Plant J. 4:1051-1061(1993).
RN [6]
RP TISSUE SPECIFICITY.
RA Thomma B.P.H.J., Broekaert W.F.;
RT "Tissue-specific expression of plant defensin genes PDF2.1 and PDF2.2 in
RT Arabidopsis thaliana.";
RL Plant Physiol. Biochem. 36:533-537(1998).
RN [7]
RP IDENTIFICATION.
RX PubMed=11437247; DOI=10.1023/a:1010664704926;
RA Vanoosthuyse V., Miege C., Dumas C., Cock J.M.;
RT "Two large Arabidopsis thaliana gene families are homologous to the
RT Brassica gene superfamily that encodes pollen coat proteins and the male
RT component of the self-incompatibility response.";
RL Plant Mol. Biol. 46:17-34(2001).
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12447532; DOI=10.1007/s00425-002-0902-6;
RA Thomma B.P.H.J., Cammue B.P., Thevissen K.;
RT "Plant defensins.";
RL Planta 216:193-202(2002).
RN [9]
RP GENE FAMILY.
RX PubMed=15955924; DOI=10.1104/pp.105.060079;
RA Silverstein K.A.T., Graham M.A., Paape T.D., VandenBosch K.A.;
RT "Genome organization of more than 300 defensin-like genes in Arabidopsis.";
RL Plant Physiol. 138:600-610(2005).
CC -!- FUNCTION: Confers broad-spectrum resistance to pathogens.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, siliques and seeds.
CC {ECO:0000269|Ref.6}.
CC -!- SIMILARITY: Belongs to the DEFL family. {ECO:0000305}.
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DR EMBL; AC005936; AAC97222.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC05548.1; -; Genomic_DNA.
DR EMBL; AK117966; BAC42603.1; -; mRNA.
DR EMBL; BT004647; AAO42893.1; -; mRNA.
DR EMBL; Z17665; CAA79029.1; -; mRNA.
DR PIR; B84433; B84433.
DR RefSeq; NP_178320.1; NM_126272.4.
DR AlphaFoldDB; Q41914; -.
DR SMR; Q41914; -.
DR STRING; 3702.AT2G02120.1; -.
DR PaxDb; Q41914; -.
DR PRIDE; Q41914; -.
DR ProteomicsDB; 224065; -.
DR EnsemblPlants; AT2G02120.1; AT2G02120.1; AT2G02120.
DR GeneID; 814743; -.
DR Gramene; AT2G02120.1; AT2G02120.1; AT2G02120.
DR KEGG; ath:AT2G02120; -.
DR Araport; AT2G02120; -.
DR TAIR; locus:2051648; AT2G02120.
DR eggNOG; ENOG502S7HM; Eukaryota.
DR HOGENOM; CLU_161668_1_2_1; -.
DR InParanoid; Q41914; -.
DR OMA; GFPGGRC; -.
DR OrthoDB; 1625543at2759; -.
DR PhylomeDB; Q41914; -.
DR PRO; PR:Q41914; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q41914; baseline and differential.
DR Genevisible; Q41914; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0006952; P:defense response; ISS:TAIR.
DR GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR CDD; cd00107; Knot1; 1.
DR Gene3D; 3.30.30.10; -; 1.
DR InterPro; IPR008176; Defensin_plant.
DR InterPro; IPR003614; Scorpion_toxin-like.
DR InterPro; IPR036574; Scorpion_toxin-like_sf.
DR PRINTS; PR00288; PUROTHIONIN.
DR SMART; SM00505; Knot1; 1.
DR SUPFAM; SSF57095; SSF57095; 1.
DR PROSITE; PS00940; GAMMA_THIONIN; 1.
PE 2: Evidence at transcript level;
KW Antimicrobial; Disulfide bond; Fungicide; Plant defense;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..30
FT /evidence="ECO:0000255"
FT CHAIN 31..77
FT /note="Defensin-like protein 4"
FT /id="PRO_0000007025"
FT DISULFID 33..77
FT /evidence="ECO:0000250"
FT DISULFID 44..64
FT /evidence="ECO:0000250"
FT DISULFID 50..71
FT /evidence="ECO:0000250"
FT DISULFID 54..73
FT /evidence="ECO:0000250"
FT CONFLICT 19
FT /note="V -> D (in Ref. 5; CAA79029)"
FT /evidence="ECO:0000305"
FT CONFLICT 69
FT /note="R -> G (in Ref. 5; CAA79029)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 77 AA; 8578 MW; FB7BE5AF727A9A45 CRC64;
MKFSMRLISA VLFLVMIFVA TGMGPVTVEA RTCASQSQRF KGKCVSDTNC ENVCHNEGFP
GGDCRGFRRR CFCTRNC
