DEF05_ARATH
ID DEF05_ARATH Reviewed; 76 AA.
AC Q9C947; A0MED6; Q1PFI2; Q8LEG6;
DT 13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 13-APR-2004, sequence version 2.
DT 03-AUG-2022, entry version 109.
DE RecName: Full=Defensin-like protein 5;
DE AltName: Full=Low-molecular-weight cysteine-rich protein 66;
DE Short=Protein LCR66;
DE AltName: Full=Plant defensin 2.4;
DE Flags: Precursor;
GN Name=PDF2.4; Synonyms=LCR66; OrderedLocusNames=At1g61070; ORFNames=T7P1.20;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP IDENTIFICATION.
RX PubMed=11437247; DOI=10.1023/a:1010664704926;
RA Vanoosthuyse V., Miege C., Dumas C., Cock J.M.;
RT "Two large Arabidopsis thaliana gene families are homologous to the
RT Brassica gene superfamily that encodes pollen coat proteins and the male
RT component of the self-incompatibility response.";
RL Plant Mol. Biol. 46:17-34(2001).
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12447532; DOI=10.1007/s00425-002-0902-6;
RA Thomma B.P.H.J., Cammue B.P., Thevissen K.;
RT "Plant defensins.";
RL Planta 216:193-202(2002).
RN [7]
RP GENE FAMILY.
RX PubMed=15955924; DOI=10.1104/pp.105.060079;
RA Silverstein K.A.T., Graham M.A., Paape T.D., VandenBosch K.A.;
RT "Genome organization of more than 300 defensin-like genes in Arabidopsis.";
RL Plant Physiol. 138:600-610(2005).
CC -!- FUNCTION: Confers broad-spectrum resistance to pathogens.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DEFL family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG51654.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=ABK28445.1; Type=Erroneous termination; Note=Extended C-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC018908; AAG51654.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002684; AEE33774.1; -; Genomic_DNA.
DR EMBL; DQ446381; ABE65729.1; -; mRNA.
DR EMBL; DQ652906; ABK28445.1; ALT_SEQ; mRNA.
DR EMBL; AY085425; AAM62652.1; -; mRNA.
DR PIR; D96636; D96636.
DR RefSeq; NP_176302.2; NM_104788.4.
DR AlphaFoldDB; Q9C947; -.
DR SMR; Q9C947; -.
DR STRING; 3702.AT1G61070.1; -.
DR PaxDb; Q9C947; -.
DR PRIDE; Q9C947; -.
DR ProteomicsDB; 224066; -.
DR EnsemblPlants; AT1G61070.1; AT1G61070.1; AT1G61070.
DR GeneID; 842399; -.
DR Gramene; AT1G61070.1; AT1G61070.1; AT1G61070.
DR KEGG; ath:AT1G61070; -.
DR Araport; AT1G61070; -.
DR TAIR; locus:2206056; AT1G61070.
DR eggNOG; ENOG502S7HM; Eukaryota.
DR HOGENOM; CLU_161668_1_2_1; -.
DR InParanoid; Q9C947; -.
DR OMA; QSNCASI; -.
DR OrthoDB; 1625543at2759; -.
DR PhylomeDB; Q9C947; -.
DR PRO; PR:Q9C947; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C947; baseline and differential.
DR Genevisible; Q9C947; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0006952; P:defense response; ISS:TAIR.
DR GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR CDD; cd00107; Knot1; 1.
DR Gene3D; 3.30.30.10; -; 1.
DR InterPro; IPR008176; Defensin_plant.
DR InterPro; IPR003614; Scorpion_toxin-like.
DR InterPro; IPR036574; Scorpion_toxin-like_sf.
DR PRINTS; PR00288; PUROTHIONIN.
DR SMART; SM00505; Knot1; 1.
DR SUPFAM; SSF57095; SSF57095; 1.
DR PROSITE; PS00940; GAMMA_THIONIN; 1.
PE 3: Inferred from homology;
KW Antimicrobial; Disulfide bond; Fungicide; Plant defense;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..29
FT /evidence="ECO:0000255"
FT CHAIN 30..76
FT /note="Defensin-like protein 5"
FT /id="PRO_0000007022"
FT DISULFID 32..76
FT /evidence="ECO:0000250"
FT DISULFID 43..63
FT /evidence="ECO:0000250"
FT DISULFID 49..70
FT /evidence="ECO:0000250"
FT DISULFID 53..72
FT /evidence="ECO:0000250"
FT CONFLICT 8
FT /note="N -> K (in Ref. 4; AAM62652)"
FT /evidence="ECO:0000305"
FT CONFLICT 24..26
FT /note="LVT -> PVR (in Ref. 4; AAM62652)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 76 AA; 8290 MW; C85F25199332B5AC CRC64;
MKVSPRLNSA LLLLFMILAT VMGLVTVEAR TCETSSNLFN GPCLSSSNCA NVCHNEGFSD
GDCRGFRRRC LCTRPC
