DEF13_ARATH
ID DEF13_ARATH Reviewed; 80 AA.
AC P30224; Q42179;
DT 01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-1998, sequence version 3.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Defensin-like protein 13;
DE AltName: Full=Anther-specific protein S18 homolog;
DE AltName: Full=Cysteine-rich antifungal protein 1;
DE Short=AFP1;
DE AltName: Full=Low-molecular-weight cysteine-rich protein 67;
DE Short=Protein LCR67;
DE AltName: Full=Plant defensin 1.1;
DE Flags: Precursor;
GN Name=PDF1.1; Synonyms=AFP1, LCR67; OrderedLocusNames=At1g75830;
GN ORFNames=T4O12.7;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX PubMed=8989885; DOI=10.2307/3870470;
RA Penninckx I.A.M.A., Eggermont K., Terras F.R.G., Thomma B.P.H.J.,
RA De Samblanx G.W., Buchala A., Metraux J.-P., Manners J.M., Broekaert W.F.;
RT "Pathogen-induced systemic activation of a plant defensin gene in
RT Arabidopsis follows a salicylic acid-independent pathway.";
RL Plant Cell 8:2309-2323(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=10080719; DOI=10.1023/a:1006127302671;
RA Williams R.W., Clark S.E., Meyerowitz E.M.;
RT "Genetic and physical characterization of a region of Arabidopsis
RT chromosome 1 containing the CLAVATA1 gene.";
RL Plant Mol. Biol. 39:171-176(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia; TISSUE=Dry seed;
RX PubMed=8580968; DOI=10.1046/j.1365-313x.1996.09010101.x;
RA Cooke R., Raynal M., Laudie M., Grellet F., Delseny M., Morris P.-C.,
RA Guerrier D., Giraudat J., Quigley F., Clabault G., Li Y.-F., Mache R.,
RA Krivitzky M., Gy I.J.-J., Kreis M., Lecharny A., Parmentier Y., Marbach J.,
RA Fleck J., Clement B., Philipps G., Herve C., Bardet C., Tremousaygue D.,
RA Lescure B., Lacomme C., Roby D., Jourjon M.-F., Chabrier P.,
RA Charpenteau J.-L., Desprez T., Amselem J., Chiapello H., Hoefte H.;
RT "Further progress towards a catalogue of all Arabidopsis genes: analysis of
RT a set of 5000 non-redundant ESTs.";
RL Plant J. 9:101-124(1996).
RN [7]
RP PROTEIN SEQUENCE OF 30-56, PYROGLUTAMATE FORMATION AT GLN-30, AND FUNCTION.
RC TISSUE=Seed;
RX PubMed=8422949; DOI=10.1016/0014-5793(93)81299-f;
RA Terras F.R.G., Torrekens S., van Leuven F., Osborn R.W., Vanderleyden J.,
RA Cammue B.P.A., Broekaert W.F.;
RT "A new family of basic cysteine-rich plant antifungal proteins from
RT Brassicaceae species.";
RL FEBS Lett. 316:233-240(1993).
RN [8]
RP IDENTIFICATION.
RX PubMed=11437247; DOI=10.1023/a:1010664704926;
RA Vanoosthuyse V., Miege C., Dumas C., Cock J.M.;
RT "Two large Arabidopsis thaliana gene families are homologous to the
RT Brassica gene superfamily that encodes pollen coat proteins and the male
RT component of the self-incompatibility response.";
RL Plant Mol. Biol. 46:17-34(2001).
RN [9]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12447532; DOI=10.1007/s00425-002-0902-6;
RA Thomma B.P.H.J., Cammue B.P., Thevissen K.;
RT "Plant defensins.";
RL Planta 216:193-202(2002).
RN [10]
RP GENE FAMILY.
RX PubMed=15955924; DOI=10.1104/pp.105.060079;
RA Silverstein K.A.T., Graham M.A., Paape T.D., VandenBosch K.A.;
RT "Genome organization of more than 300 defensin-like genes in Arabidopsis.";
RL Plant Physiol. 138:600-610(2005).
RN [11]
RP FUNCTION.
RX PubMed=17180735; DOI=10.1007/s11248-006-9057-8;
RA Sels J., Delaure S.L., Aerts A.M., Proost P., Cammue B.P.A.,
RA De Bolle M.F.C.;
RT "Use of a PTGS-MAR expression system for efficient in planta production of
RT bioactive Arabidopsis thaliana plant defensins.";
RL Transgenic Res. 16:531-538(2007).
CC -!- FUNCTION: Confers broad-spectrum resistance to pathogens. Possesses
CC antifungal activity sensitive to inorganic cations in vitro.
CC {ECO:0000269|PubMed:17180735, ECO:0000269|PubMed:8422949}.
CC -!- SUBUNIT: Forms oligomers in its native state.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Expressed predominantly in siliques and dry seeds.
CC {ECO:0000269|PubMed:8989885}.
CC -!- SIMILARITY: Belongs to the DEFL family. {ECO:0000305}.
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DR EMBL; AF049870; AAD02502.1; -; Genomic_DNA.
DR EMBL; AC007396; AAF26754.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35763.1; -; Genomic_DNA.
DR EMBL; AY052236; AAK97706.1; -; mRNA.
DR EMBL; AY060506; AAL31119.1; -; mRNA.
DR EMBL; X91916; CAA63009.1; -; mRNA.
DR EMBL; Z27258; CAA81770.1; -; mRNA.
DR EMBL; Z29957; CAA82845.1; -; mRNA.
DR PIR; F96787; F96787.
DR PIR; S28995; S28995.
DR RefSeq; NP_565119.1; NM_106233.4.
DR AlphaFoldDB; P30224; -.
DR BMRB; P30224; -.
DR SMR; P30224; -.
DR STRING; 3702.AT1G75830.1; -.
DR PaxDb; P30224; -.
DR PRIDE; P30224; -.
DR ProteomicsDB; 224665; -.
DR EnsemblPlants; AT1G75830.1; AT1G75830.1; AT1G75830.
DR GeneID; 843916; -.
DR Gramene; AT1G75830.1; AT1G75830.1; AT1G75830.
DR KEGG; ath:AT1G75830; -.
DR Araport; AT1G75830; -.
DR TAIR; locus:2204365; AT1G75830.
DR eggNOG; ENOG502SQS4; Eukaryota.
DR HOGENOM; CLU_161668_3_0_1; -.
DR InParanoid; P30224; -.
DR OMA; NRENAHT; -.
DR OrthoDB; 1565340at2759; -.
DR PhylomeDB; P30224; -.
DR PRO; PR:P30224; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; P30224; baseline and differential.
DR Genevisible; P30224; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0006952; P:defense response; ISS:TAIR.
DR GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR Gene3D; 3.30.30.10; -; 1.
DR InterPro; IPR008176; Defensin_plant.
DR InterPro; IPR003614; Scorpion_toxin-like.
DR InterPro; IPR036574; Scorpion_toxin-like_sf.
DR SMART; SM00505; Knot1; 1.
DR SUPFAM; SSF57095; SSF57095; 1.
DR PROSITE; PS00940; GAMMA_THIONIN; 1.
PE 1: Evidence at protein level;
KW Antimicrobial; Direct protein sequencing; Disulfide bond; Fungicide;
KW Plant defense; Pyrrolidone carboxylic acid; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..29
FT /evidence="ECO:0000269|PubMed:8422949"
FT CHAIN 30..80
FT /note="Defensin-like protein 13"
FT /id="PRO_0000007019"
FT MOD_RES 30
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000269|PubMed:8422949"
FT DISULFID 33..80
FT /evidence="ECO:0000250"
FT DISULFID 44..65
FT /evidence="ECO:0000250"
FT DISULFID 50..74
FT /evidence="ECO:0000250"
FT DISULFID 54..76
FT /evidence="ECO:0000250"
FT CONFLICT 33
FT /note="C -> F (in Ref. 6; CAA81770)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 80 AA; 8709 MW; 99F34A8DA360441F CRC64;
MAKSATIVTL FFAALVFFAA LEAPMVVEAQ KLCERPSGTW SGVCGNSNAC KNQCINLEKA
RHGSCNYVFP AHKCICYFPC
