DEF14_ARATH
ID DEF14_ARATH Reviewed; 80 AA.
AC O80995;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Defensin-like protein 14;
DE AltName: Full=Cysteine-rich antifungal protein At2g26010;
DE AltName: Full=Plant defensin 1.3;
DE Flags: Precursor;
GN Name=PDF1.3; OrderedLocusNames=At2g26010; ORFNames=T19L18.18;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12447532; DOI=10.1007/s00425-002-0902-6;
RA Thomma B.P.H.J., Cammue B.P., Thevissen K.;
RT "Plant defensins.";
RL Planta 216:193-202(2002).
RN [4]
RP GENE FAMILY.
RX PubMed=15955924; DOI=10.1104/pp.105.060079;
RA Silverstein K.A.T., Graham M.A., Paape T.D., VandenBosch K.A.;
RT "Genome organization of more than 300 defensin-like genes in Arabidopsis.";
RL Plant Physiol. 138:600-610(2005).
RN [5]
RP FUNCTION.
RX PubMed=17180735; DOI=10.1007/s11248-006-9057-8;
RA Sels J., Delaure S.L., Aerts A.M., Proost P., Cammue B.P.A.,
RA De Bolle M.F.C.;
RT "Use of a PTGS-MAR expression system for efficient in planta production of
RT bioactive Arabidopsis thaliana plant defensins.";
RL Transgenic Res. 16:531-538(2007).
CC -!- FUNCTION: Confers broad-spectrum resistance to pathogens. Has
CC antifungal activity in vitro. {ECO:0000269|PubMed:17180735}.
CC -!- INTERACTION:
CC O80995; A4FVP6: NAC016; NbExp=3; IntAct=EBI-25522494, EBI-25522702;
CC O80995; Q94F58: NAC089; NbExp=3; IntAct=EBI-25522494, EBI-2319707;
CC O80995; F4JN35: NTL9; NbExp=3; IntAct=EBI-25522494, EBI-4426607;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DEFL family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC004747; AAC31234.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC07783.1; -; Genomic_DNA.
DR PIR; T02622; T02622.
DR RefSeq; NP_180171.1; NM_128160.3.
DR AlphaFoldDB; O80995; -.
DR SMR; O80995; -.
DR BioGRID; 2494; 3.
DR IntAct; O80995; 3.
DR STRING; 3702.AT2G26010.1; -.
DR PaxDb; O80995; -.
DR PRIDE; O80995; -.
DR ProteomicsDB; 224068; -.
DR EnsemblPlants; AT2G26010.1; AT2G26010.1; AT2G26010.
DR GeneID; 817142; -.
DR Gramene; AT2G26010.1; AT2G26010.1; AT2G26010.
DR KEGG; ath:AT2G26010; -.
DR Araport; AT2G26010; -.
DR TAIR; locus:2057448; AT2G26010.
DR eggNOG; ENOG502SQS4; Eukaryota.
DR HOGENOM; CLU_161668_3_0_1; -.
DR InParanoid; O80995; -.
DR OMA; RCICYFQ; -.
DR OrthoDB; 1565340at2759; -.
DR PhylomeDB; O80995; -.
DR PRO; PR:O80995; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O80995; baseline and differential.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0099503; C:secretory vesicle; HDA:TAIR.
DR GO; GO:0006952; P:defense response; ISS:TAIR.
DR GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR CDD; cd00107; Knot1; 1.
DR Gene3D; 3.30.30.10; -; 1.
DR InterPro; IPR008176; Defensin_plant.
DR InterPro; IPR003614; Scorpion_toxin-like.
DR InterPro; IPR036574; Scorpion_toxin-like_sf.
DR SMART; SM00505; Knot1; 1.
DR SUPFAM; SSF57095; SSF57095; 1.
DR PROSITE; PS00940; GAMMA_THIONIN; 1.
PE 2: Evidence at transcript level;
KW Antimicrobial; Disulfide bond; Fungicide; Plant defense;
KW Pyrrolidone carboxylic acid; Reference proteome; Secreted; Signal.
FT SIGNAL 1..29
FT /evidence="ECO:0000250"
FT CHAIN 30..80
FT /note="Defensin-like protein 14"
FT /id="PRO_0000007020"
FT MOD_RES 30
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000250|UniProtKB:P30224"
FT DISULFID 33..80
FT /evidence="ECO:0000250"
FT DISULFID 44..65
FT /evidence="ECO:0000250"
FT DISULFID 50..74
FT /evidence="ECO:0000250"
FT DISULFID 54..76
FT /evidence="ECO:0000250"
SQ SEQUENCE 80 AA; 8580 MW; 99E1E0D4443AD67B CRC64;
MAKSAAIITF LFAALVLFAA FEAPIMVEAQ KLCEKPSGTW SGVCGNSNAC KNQCINLEGA
KHGSCNYVFP AHKCICYFPC
