DEF16_ARATH
ID DEF16_ARATH Reviewed; 80 AA.
AC Q9FI23; P82786;
DT 26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Defensin-like protein 16;
DE AltName: Full=Low-molecular-weight cysteine-rich protein 77;
DE Short=Protein LCR77;
DE AltName: Full=Plant defensin 1.2;
DE AltName: Full=Plant defensin 1.2a;
DE Flags: Precursor;
GN Name=PDF1.2A; Synonyms=LCR77, PDF1.2; OrderedLocusNames=At5g44420;
GN ORFNames=MFC16.8;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=8989885; DOI=10.2307/3870470;
RA Penninckx I.A.M.A., Eggermont K., Terras F.R.G., Thomma B.P.H.J.,
RA De Samblanx G.W., Buchala A., Metraux J.-P., Manners J.M., Broekaert W.F.;
RT "Pathogen-induced systemic activation of a plant defensin gene in
RT Arabidopsis follows a salicylic acid-independent pathway.";
RL Plant Cell 8:2309-2323(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10470850; DOI=10.1093/dnares/6.3.183;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence
RT features of the regions of 1,011,550 bp covered by seventeen P1 and TAC
RT clones.";
RL DNA Res. 6:183-195(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=9001391; DOI=10.1016/s0014-5793(96)01378-6;
RA Epple P., Apel K., Bohlmann H.;
RT "ESTs reveal a multigene family for plant defensins in Arabidopsis
RT thaliana.";
RL FEBS Lett. 400:168-172(1997).
RN [6]
RP INDUCTION.
RX PubMed=9836748; DOI=10.2307/3870787;
RA Penninckx I.A.M.A., Thomma B.P.H.J., Buchala A., Metraux J.-P.,
RA Broekaert W.F.;
RT "Concomitant activation of jasmonate and ethylene response pathways is
RT required for induction of a plant defensin gene in Arabidopsis.";
RL Plant Cell 10:2103-2113(1998).
RN [7]
RP IDENTIFICATION.
RX PubMed=11437247; DOI=10.1023/a:1010664704926;
RA Vanoosthuyse V., Miege C., Dumas C., Cock J.M.;
RT "Two large Arabidopsis thaliana gene families are homologous to the
RT Brassica gene superfamily that encodes pollen coat proteins and the male
RT component of the self-incompatibility response.";
RL Plant Mol. Biol. 46:17-34(2001).
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12447532; DOI=10.1007/s00425-002-0902-6;
RA Thomma B.P.H.J., Cammue B.P., Thevissen K.;
RT "Plant defensins.";
RL Planta 216:193-202(2002).
RN [9]
RP GENE FAMILY.
RX PubMed=15955924; DOI=10.1104/pp.105.060079;
RA Silverstein K.A.T., Graham M.A., Paape T.D., VandenBosch K.A.;
RT "Genome organization of more than 300 defensin-like genes in Arabidopsis.";
RL Plant Physiol. 138:600-610(2005).
RN [10]
RP INDUCTION.
RX PubMed=18467450; DOI=10.1104/pp.108.117523;
RA Pre M., Atallah M., Champion A., De Vos M., Pieterse C.M.J., Memelink J.;
RT "The AP2/ERF domain transcription factor ORA59 integrates jasmonic acid and
RT ethylene signals in plant defense.";
RL Plant Physiol. 147:1347-1357(2008).
CC -!- FUNCTION: Confers broad-spectrum resistance to pathogens. Has
CC antifungal activity in vitro. {ECO:0000269|PubMed:8989885}.
CC -!- INTERACTION:
CC Q9FI23; Q94BX2: At3g50910; NbExp=3; IntAct=EBI-25516740, EBI-4424796;
CC Q9FI23; Q9M126: NAC69; NbExp=3; IntAct=EBI-25516740, EBI-15191931;
CC Q9FI23; F4JN35: NTL9; NbExp=3; IntAct=EBI-25516740, EBI-4426607;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Predominantly expressed in leaves.
CC {ECO:0000269|PubMed:8989885, ECO:0000269|PubMed:9001391}.
CC -!- INDUCTION: By pathogens, methyl jasmonate and ethylene. Up-regulated by
CC ORA59/ERF094. {ECO:0000269|PubMed:18467450, ECO:0000269|PubMed:8989885,
CC ECO:0000269|PubMed:9836748}.
CC -!- SIMILARITY: Belongs to the DEFL family. {ECO:0000305}.
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DR EMBL; AB017065; BAB09149.1; -; Genomic_DNA.
DR EMBL; CP002688; AED95107.1; -; Genomic_DNA.
DR EMBL; AY063779; AAL36086.1; -; mRNA.
DR EMBL; AY133787; AAM91721.1; -; mRNA.
DR RefSeq; NP_199255.1; NM_123809.4.
DR AlphaFoldDB; Q9FI23; -.
DR SMR; Q9FI23; -.
DR BioGRID; 19719; 3.
DR IntAct; Q9FI23; 3.
DR STRING; 3702.AT5G44420.1; -.
DR PaxDb; Q9FI23; -.
DR PRIDE; Q9FI23; -.
DR EnsemblPlants; AT5G44420.1; AT5G44420.1; AT5G44420.
DR GeneID; 834469; -.
DR Gramene; AT5G44420.1; AT5G44420.1; AT5G44420.
DR KEGG; ath:AT5G44420; -.
DR Araport; AT5G44420; -.
DR TAIR; locus:2163421; AT5G44420.
DR eggNOG; ENOG502SQS4; Eukaryota.
DR HOGENOM; CLU_161668_3_0_1; -.
DR InParanoid; Q9FI23; -.
DR OMA; EANNACK; -.
DR OrthoDB; 1565340at2759; -.
DR PhylomeDB; Q9FI23; -.
DR PRO; PR:Q9FI23; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FI23; baseline and differential.
DR Genevisible; Q9FI23; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0006952; P:defense response; TAS:TAIR.
DR GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR GO; GO:0009861; P:jasmonic acid and ethylene-dependent systemic resistance; TAS:TAIR.
DR GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR GO; GO:0009723; P:response to ethylene; IEP:TAIR.
DR GO; GO:0009625; P:response to insect; IEP:TAIR.
DR GO; GO:0009753; P:response to jasmonic acid; IEP:TAIR.
DR CDD; cd00107; Knot1; 1.
DR Gene3D; 3.30.30.10; -; 1.
DR InterPro; IPR008176; Defensin_plant.
DR InterPro; IPR003614; Scorpion_toxin-like.
DR InterPro; IPR036574; Scorpion_toxin-like_sf.
DR SMART; SM00505; Knot1; 1.
DR SUPFAM; SSF57095; SSF57095; 1.
DR PROSITE; PS00940; GAMMA_THIONIN; 1.
PE 1: Evidence at protein level;
KW Antimicrobial; Disulfide bond; Fungicide; Plant defense;
KW Pyrrolidone carboxylic acid; Reference proteome; Secreted; Signal.
FT SIGNAL 1..29
FT /evidence="ECO:0000255"
FT CHAIN 30..80
FT /note="Defensin-like protein 16"
FT /id="PRO_0000007031"
FT MOD_RES 30
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000250|UniProtKB:P30224"
FT DISULFID 33..80
FT /evidence="ECO:0000250"
FT DISULFID 44..65
FT /evidence="ECO:0000250"
FT DISULFID 50..74
FT /evidence="ECO:0000250"
FT DISULFID 54..76
FT /evidence="ECO:0000250"
SQ SEQUENCE 80 AA; 8518 MW; 2D0DAFB38E3B6321 CRC64;
MAKFASIITL IFAALVLFAA FDAPAMVEAQ KLCEKPSGTW SGVCGNSNAC KNQCINLEGA
KHGSCNYVFP AHKCICYVPC
