ID   DEF1_BRANA              Reviewed;          44 AA.
AC   P69240; P30225; Q41163;
DT   15-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-FEB-2005, sequence version 1.
DT   25-MAY-2022, entry version 47.
DE   RecName: Full=Defensin-like protein 1;
DE   AltName: Full=Cysteine-rich antifungal protein 1;
DE            Short=AFP1;
DE   Flags: Fragment;
GN   Name=AFP1;
OS   Brassica napus (Rape).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Brassiceae; Brassica.
OX   NCBI_TaxID=3708;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   TISSUE=Seed;
RX   PubMed=8422949; DOI=10.1016/0014-5793(93)81299-f;
RA   Terras F.R.G., Torrekens S., van Leuven F., Osborn R.W., Vanderleyden J.,
RA   Cammue B.P.A., Broekaert W.F.;
RT   "A new family of basic cysteine-rich plant antifungal proteins from
RT   Brassicaceae species.";
RL   FEBS Lett. 316:233-240(1993).
CC   -!- FUNCTION: Possesses antifungal activity sensitive to inorganic cations.
CC   -!- SUBUNIT: Forms oligomers in its native state.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the DEFL family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   AlphaFoldDB; P69240; -.
DR   BMRB; P69240; -.
DR   SMR; P69240; -.
DR   EnsemblPlants; CDY39063; CDY39063; GSBRNA2T00067211001.
DR   Gramene; CDY39063; CDY39063; GSBRNA2T00067211001.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR   Gene3D; 3.30.30.10; -; 1.
DR   InterPro; IPR008176; Defensin_plant.
DR   InterPro; IPR003614; Scorpion_toxin-like.
DR   InterPro; IPR036574; Scorpion_toxin-like_sf.
DR   SMART; SM00505; Knot1; 1.
DR   SUPFAM; SSF57095; SSF57095; 1.
DR   PROSITE; PS00940; GAMMA_THIONIN; 1.
PE   1: Evidence at protein level;
KW   Antimicrobial; Direct protein sequencing; Disulfide bond; Fungicide;
KW   Plant defense; Pyrrolidone carboxylic acid; Secreted.
FT   CHAIN           1..>44
FT                   /note="Defensin-like protein 1"
FT                   /id="PRO_0000074235"
FT   MOD_RES         1
FT                   /note="Pyrrolidone carboxylic acid"
FT                   /evidence="ECO:0000250|UniProtKB:P30231"
FT   DISULFID        15..36
FT                   /evidence="ECO:0000250"
FT   NON_TER         44
SQ   SEQUENCE   44 AA;  4865 MW;  19135F46F68C39D6 CRC64;
     QKLCERPSGT WSGVCGNNNA CKNQCINLEK ARHGSCNYVF PAHK