DEF1_CANAL
ID DEF1_CANAL Reviewed; 738 AA.
AC Q5AMM4; A0A1D8PLC5;
DT 08-MAR-2011, integrated into UniProtKB/Swiss-Prot.
DT 10-MAY-2017, sequence version 2.
DT 03-AUG-2022, entry version 68.
DE RecName: Full=RNA polymerase II degradation factor 1;
GN Name=DEF1; OrderedLocusNames=CAALFM_C401720CA;
GN ORFNames=CaO19.12092, CaO19.4622;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
CC -!- FUNCTION: RNA polymerase II degradation factor recruits the
CC ubiquitination machinery to the RNA polymerase II for
CC polyubiquitination, removal and degradation, when RAD26 fails to
CC efficiently displace stalled RNA polymerase II. Also involved in
CC telomere length regulation (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome, telomere
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DEF1 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AOW28944.1; Type=Miscellaneous discrepancy; Note=Translation C-terminally extended.; Evidence={ECO:0000305};
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DR EMBL; CP017626; AOW28944.1; ALT_SEQ; Genomic_DNA.
DR RefSeq; XP_722694.2; XM_717601.2.
DR AlphaFoldDB; Q5AMM4; -.
DR SMR; Q5AMM4; -.
DR BioGRID; 1218646; 1.
DR STRING; 237561.Q5AMM4; -.
DR PRIDE; Q5AMM4; -.
DR GeneID; 3635589; -.
DR KEGG; cal:CAALFM_C401720CA; -.
DR CGD; CAL0000185361; orf19.12092.
DR eggNOG; ENOG502S359; Eukaryota.
DR HOGENOM; CLU_043289_0_0_1; -.
DR InParanoid; Q5AMM4; -.
DR OrthoDB; 1596012at2759; -.
DR PRO; PR:Q5AMM4; -.
DR Proteomes; UP000000559; Chromosome 4.
DR GO; GO:0000781; C:chromosome, telomeric region; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0043130; F:ubiquitin binding; IEA:InterPro.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR InterPro; IPR003892; CUE.
DR Pfam; PF02845; CUE; 1.
DR PROSITE; PS51140; CUE; 1.
PE 3: Inferred from homology;
KW Chromosome; DNA damage; DNA repair; DNA-binding; Nucleus;
KW Reference proteome; Telomere; Ubl conjugation pathway.
FT CHAIN 1..738
FT /note="RNA polymerase II degradation factor 1"
FT /id="PRO_0000405663"
FT DOMAIN 23..66
FT /note="CUE"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00468"
FT REGION 1..25
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 73..306
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 372..460
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 587..711
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 89..130
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 147..178
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 213..227
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 230..244
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 391..460
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 738 AA; 80992 MW; 9C7F1D94A744ADF8 CRC64;
MSTYRKTYKN QSKRFNNGNN SNSTSTELTN LVEMFPDWEA DELQGLLSEN DNSLEIVIDL
IVNNKVSKWE PIKKEKHKKK EHKDDTTDSV TGNANGASNS GSSNSTTASG DRRLNNKAKA
STSSRPPKRQ QHPNAAHKKN EKTERSKAST VSTTTTSATS SVTKESVPPS NSWAAALSND
KPKKQETKSE ASESIPSSND GGDAETVPEV EQAETLEESH QEPENVEEPI ESVSEQSQPE
NTKPVLKSAA IPEPKQGSWA SAIAPKTKPK PKTVSKPVPQ PEESKPEEHI PVQEEAQPVE
SILEDKPAVE AVQPVQPPVE DEPVEAPAAS SVAAVADASF SEPTASSIID QQPQVVLPTT
QQQVDSVGIS FGSLSVDAGE PKEAAEVIQD ETISEQVQPE QQQQSQQQPL QEQQRYGLYN
QQPTQQRYQQ NNYQQQGQYS KQQQQQQQQQ TSQPTQQQQQ YDYYGQYQQQ QYPQQTAQPG
AQFGGYPGFD YNAYNQQAFA AAAAASPAAS HVATIGGGVG GYNQYAQQAN SADSTQSPIV
NQSTLQQQQA AVLAAQQQQQ QLPTPFGYPA YYNYYYNNPY FNAGGLGSTG FSAPQQGTQQ
GTQQQQSQQS GQQQQQVSSA NQQGSVSSNS FGAQQQYYNP NQFNSRYPGY SYPPQQQNQQ
SSQHTSGQSQ QGAQTTSDGA SDGQQTSQQG QSSQQPQQQQ QQHAQPVPQQ HMMPQYGAYQ
QYPQYGYQDS NQYRGGWY
