DEF1_CANTT
ID DEF1_CANTT Reviewed; 769 AA.
AC C5M2B8;
DT 08-MAR-2011, integrated into UniProtKB/Swiss-Prot.
DT 28-JUL-2009, sequence version 1.
DT 25-MAY-2022, entry version 29.
DE RecName: Full=RNA polymerase II degradation factor 1;
GN Name=DEF1; ORFNames=CTRG_00207;
OS Candida tropicalis (strain ATCC MYA-3404 / T1) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=294747;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC MYA-3404 / T1;
RX PubMed=19465905; DOI=10.1038/nature08064;
RA Butler G., Rasmussen M.D., Lin M.F., Santos M.A.S., Sakthikumar S.,
RA Munro C.A., Rheinbay E., Grabherr M., Forche A., Reedy J.L., Agrafioti I.,
RA Arnaud M.B., Bates S., Brown A.J.P., Brunke S., Costanzo M.C.,
RA Fitzpatrick D.A., de Groot P.W.J., Harris D., Hoyer L.L., Hube B.,
RA Klis F.M., Kodira C., Lennard N., Logue M.E., Martin R., Neiman A.M.,
RA Nikolaou E., Quail M.A., Quinn J., Santos M.C., Schmitzberger F.F.,
RA Sherlock G., Shah P., Silverstein K.A.T., Skrzypek M.S., Soll D.,
RA Staggs R., Stansfield I., Stumpf M.P.H., Sudbery P.E., Srikantha T.,
RA Zeng Q., Berman J., Berriman M., Heitman J., Gow N.A.R., Lorenz M.C.,
RA Birren B.W., Kellis M., Cuomo C.A.;
RT "Evolution of pathogenicity and sexual reproduction in eight Candida
RT genomes.";
RL Nature 459:657-662(2009).
CC -!- FUNCTION: RNA polymerase II degradation factor recruits the
CC ubiquitination machinery to the RNA polymerase II for
CC polyubiquitination, removal and degradation, when RAD26 fails to
CC efficiently displace stalled RNA polymerase II. Also involved in
CC telomere length regulation (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome, telomere
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the DEF1 family. {ECO:0000305}.
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DR EMBL; GG692395; EER35469.1; -; Genomic_DNA.
DR RefSeq; XP_002545427.1; XM_002545381.1.
DR AlphaFoldDB; C5M2B8; -.
DR SMR; C5M2B8; -.
DR STRING; 5482.XP_002545427.1; -.
DR EnsemblFungi; EER35469; EER35469; CTRG_00207.
DR GeneID; 8298144; -.
DR KEGG; ctp:CTRG_00207; -.
DR VEuPathDB; FungiDB:CTRG_00207; -.
DR eggNOG; ENOG502S359; Eukaryota.
DR HOGENOM; CLU_438800_0_0_1; -.
DR OrthoDB; 1596012at2759; -.
DR Proteomes; UP000002037; Unassembled WGS sequence.
DR GO; GO:0000781; C:chromosome, telomeric region; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0043130; F:ubiquitin binding; IEA:InterPro.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR InterPro; IPR003892; CUE.
DR Pfam; PF02845; CUE; 1.
DR PROSITE; PS51140; CUE; 1.
PE 3: Inferred from homology;
KW Chromosome; DNA damage; DNA repair; DNA-binding; Nucleus;
KW Reference proteome; Telomere; Ubl conjugation pathway.
FT CHAIN 1..769
FT /note="RNA polymerase II degradation factor 1"
FT /id="PRO_0000405665"
FT DOMAIN 24..67
FT /note="CUE"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00468"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 66..368
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 381..472
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 540..559
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 602..769
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 69..89
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 90..129
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 145..176
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 182..209
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 210..241
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 285..303
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 315..368
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 399..472
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 604..762
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 769 AA; 84243 MW; 7EF6CFFD3F8D4AC4 CRC64;
MSTFRKSYKN QSKRFNNSHS NSSSTSVELT NLVEMFPDWE ADELQGLLSE NDNSLEIVID
LIVNNKVSKW EPIKKEKHKK KEKETEDHNS STNGSTNSAS TSSTAPSSDR KPYNKSKPQS
SSTRPPKKQH ANANYKKGGE KSAEKPKPTS TTSSTTTTPV QKETTPATNS WAAALSNDKP
KKSVATTEES EPVEHAEEDG AKAQQEEQET VAELDNTTTT NGEQQEQQPE EETTQESQAP
VEQAKPVLKE AAIPEPKQGS WASAITPKTV KPKAKPAPKP VSQPQPPKEE EEKEEKEPVV
EEQVSEPVES IAEETVAEVS QSIPSEEPIE SPVATATTTT AATTTEPSIV EQPQQQQQPQ
VVLPTSQQQV NSVGISFGSL SIQEESKDAE VPREEVIAEQ TSQPEQTQQQ QQQPIQDQQR
YGLYNQQQTQ QRYQQQQQSA YQQQPQQQQQ QNQYSKQQQQ QQQAPIQQQT AQQQYDYYNQ
FQQQYPQQTA QPGAQFGGYP GFDYSGYNQQ AFAAASPAAN HIATLGGSAG VGGGYGQYAQ
QAGSTDSSHS PITNQSTLQQ QQAAAAALNA QQQQQQMPTP FGYPAYYNYY YNNPYFNAGG
LGSTGFGAPQ QGNQQGQQPT SSQGASQQGQ AQPQVPSAGS QQGGASMGSN GFGGQQQYYN
PNQFNNRYPG YSYPPQQQQQ QQQPVQQNAG QSQQGSSQSA SNSEGASDGQ QSSSQGQQGQ
QGQGPQGVQQ GQGQQPPVPQ QHMMPQYGGY QQYPQYGYQD SNQYRGGWY
