ID   DEF1_CRAVI              Reviewed;          38 AA.
AC   P85008;
DT   31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 32.
DE   RecName: Full=Defensin-1;
DE   AltName: Full=American oyster defensin;
DE            Short=AOD;
OS   Crassostrea virginica (Eastern oyster).
OC   Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Bivalvia;
OC   Autobranchia; Pteriomorphia; Ostreida; Ostreoidea; Ostreidae; Crassostrea.
OX   NCBI_TaxID=6565;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, DISULFIDE BONDS, AND MASS SPECTROMETRY.
RC   TISSUE=Gill {ECO:0000269|PubMed:16297885};
RX   PubMed=16297885; DOI=10.1016/j.bbrc.2005.11.013;
RA   Seo J.-K., Crawford J.M., Stone K.L., Noga E.J.;
RT   "Purification of a novel arthropod defensin from the American oyster,
RT   Crassostrea virginica.";
RL   Biochem. Biophys. Res. Commun. 338:1998-2004(2005).
CC   -!- FUNCTION: Has antibacterial activity against the Gram-positive bacteria
CC       L.lactis and S.aureus, and against the Gram-negative bacteria E.coli
CC       D32 and V.parahemolyticus. {ECO:0000269|PubMed:16297885}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:16297885}.
CC   -!- MASS SPECTROMETRY: Mass=4265.0; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:16297885};
CC   -!- SIMILARITY: Belongs to the invertebrate defensin family. Type 2
CC       subfamily. {ECO:0000255|PROSITE-ProRule:PRU00710}.
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DR   AlphaFoldDB; P85008; -.
DR   SMR; P85008; -.
DR   Proteomes; UP000694844; Genome assembly.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0050829; P:defense response to Gram-negative bacterium; IDA:UniProtKB.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
DR   GO; GO:0045087; P:innate immune response; IDA:UniProtKB.
DR   Gene3D; 3.30.30.10; -; 1.
DR   InterPro; IPR001542; Defensin_invertebrate/fungal.
DR   InterPro; IPR036574; Scorpion_toxin-like_sf.
DR   Pfam; PF01097; Defensin_2; 1.
DR   SUPFAM; SSF57095; SSF57095; 1.
DR   PROSITE; PS51378; INVERT_DEFENSINS; 1.
PE   1: Evidence at protein level;
KW   Antibiotic; Antimicrobial; Defensin; Direct protein sequencing;
KW   Disulfide bond; Immunity; Innate immunity; Reference proteome; Secreted.
FT   PEPTIDE         1..38
FT                   /note="Defensin-1"
FT                   /evidence="ECO:0000269|PubMed:16297885"
FT                   /id="PRO_0000257983"
FT   DISULFID        4..25
FT                   /evidence="ECO:0000250|UniProtKB:I1T3C7"
FT   DISULFID        11..33
FT                   /evidence="ECO:0000250|UniProtKB:I1T3C7"
FT   DISULFID        15..35
FT                   /evidence="ECO:0000250|UniProtKB:I1T3C7"
SQ   SEQUENCE   38 AA;  4271 MW;  7222BFDAFC4D095B CRC64;
     GFGCPWNRYQ CHSHCRSIGR LGGYCAGSLR LTCTCYRS